Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria
Isolates of Colletotrichum gloeosporioides obtained from yam-based cropping systems in Nigeria, previously characterized on the basis of morphology, virulence and rDNA internal transcribed spacer (ITS) sequence variation were further compared for vegetative compatibility (VC). Chlorate-resistant nit...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Wiley
2004
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| Online Access: | https://hdl.handle.net/10568/96361 |
| _version_ | 1855519117603241984 |
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| author | Abang, Mathew M. Hoffmann, P. Winter, S. Green, K.R. Wolf, G.A. |
| author_browse | Abang, Mathew M. Green, K.R. Hoffmann, P. Winter, S. Wolf, G.A. |
| author_facet | Abang, Mathew M. Hoffmann, P. Winter, S. Green, K.R. Wolf, G.A. |
| author_sort | Abang, Mathew M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Isolates of Colletotrichum gloeosporioides obtained from yam-based cropping systems in Nigeria, previously characterized on the basis of morphology, virulence and rDNA internal transcribed spacer (ITS) sequence variation were further compared for vegetative compatibility (VC). Chlorate-resistant nitrate non-utilizing (nit) mutants were generated from the isolates and used in complementation (heterokaryon) tests. Tests of VC between complementary mutants from different isolates indicated the presence of several genotypes within a single field, suggesting limited clonal spread. In some cases, isolates obtained from the same lesion were observed to belong to different vegetative compatibility groups (VCGs). No compatibility was observed between isolates of the highly virulent slow-growing grey (SGG), the moderately virulent fast-growing salmon (FGS) and the avirulent/weakly virulent fast-growing grey (FGG) strains. Forty-one C. gloeosporioides isolates belonged to 28 VCGs, giving a genotype diversity estimate of 0.68. This diversity confirmed the high variability of the pathogen population as revealed by previous characterization studies, however, a correlation between VCGs and isolate groupings based on morphology and virulence was not found. The finding that an isolate from weed was compatible with yam isolates indicated that transfer of important traits, such as virulence, may take place between isolates from yam and non-yam hosts. The VCG diversity revealed by this study suggests that in addition to asexual reproduction, sexual reproduction may play an important role in the epidemiology of anthracnose on yam. |
| format | Journal Article |
| id | CGSpace96361 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace963612024-08-27T10:35:15Z Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria Abang, Mathew M. Hoffmann, P. Winter, S. Green, K.R. Wolf, G.A. anthracnosis dioscorea yams genetics physiology Isolates of Colletotrichum gloeosporioides obtained from yam-based cropping systems in Nigeria, previously characterized on the basis of morphology, virulence and rDNA internal transcribed spacer (ITS) sequence variation were further compared for vegetative compatibility (VC). Chlorate-resistant nitrate non-utilizing (nit) mutants were generated from the isolates and used in complementation (heterokaryon) tests. Tests of VC between complementary mutants from different isolates indicated the presence of several genotypes within a single field, suggesting limited clonal spread. In some cases, isolates obtained from the same lesion were observed to belong to different vegetative compatibility groups (VCGs). No compatibility was observed between isolates of the highly virulent slow-growing grey (SGG), the moderately virulent fast-growing salmon (FGS) and the avirulent/weakly virulent fast-growing grey (FGG) strains. Forty-one C. gloeosporioides isolates belonged to 28 VCGs, giving a genotype diversity estimate of 0.68. This diversity confirmed the high variability of the pathogen population as revealed by previous characterization studies, however, a correlation between VCGs and isolate groupings based on morphology and virulence was not found. The finding that an isolate from weed was compatible with yam isolates indicated that transfer of important traits, such as virulence, may take place between isolates from yam and non-yam hosts. The VCG diversity revealed by this study suggests that in addition to asexual reproduction, sexual reproduction may play an important role in the epidemiology of anthracnose on yam. 2004-01 2018-08-09T06:40:32Z 2018-08-09T06:40:32Z Journal Article https://hdl.handle.net/10568/96361 en Limited Access Wiley Abang, M.M., Hoffmann, P., Winter, S., Green, K.R. & Wolf, G.A. (2004). Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria. Journal of Phytopathology, 152(1), 21-27. |
| spellingShingle | anthracnosis dioscorea yams genetics physiology Abang, Mathew M. Hoffmann, P. Winter, S. Green, K.R. Wolf, G.A. Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria |
| title | Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria |
| title_full | Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria |
| title_fullStr | Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria |
| title_full_unstemmed | Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria |
| title_short | Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria |
| title_sort | vegetative compatibility among isolates of colletotrichum gloeosporioides from yam dioscorea spp in nigeria |
| topic | anthracnosis dioscorea yams genetics physiology |
| url | https://hdl.handle.net/10568/96361 |
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