Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California
Interactions between pathogenic and nonpathogenic fungal species in the tree canopy are complex and can determine if disease will manifest in the plant and in other organisms such as honey bees. Seasonal dynamics of fungi were studied in an almond orchard in California where experimental release of...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Public Library of Science
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/96134 |
| _version_ | 1855523146322411520 |
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| author | Ortega Beltran, A. Moral, J. Puckett, R.D. Morgan, D.P. Cotty, P.J. Michailides, T. |
| author_browse | Cotty, P.J. Michailides, T. Moral, J. Morgan, D.P. Ortega Beltran, A. Puckett, R.D. |
| author_facet | Ortega Beltran, A. Moral, J. Puckett, R.D. Morgan, D.P. Cotty, P.J. Michailides, T. |
| author_sort | Ortega Beltran, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Interactions between pathogenic and nonpathogenic fungal species in the tree canopy are complex and can determine if disease will manifest in the plant and in other organisms such as honey bees. Seasonal dynamics of fungi were studied in an almond orchard in California where experimental release of the atoxigenic biopesticide Aspergillus flavus AF36 to displace toxigenic Aspergillus strains has been conducted for five years. The presence of the vegetative compatibility group (VCG) YV36, to which AF36 belongs, in the blossoms, and the honey bees that attend these blossoms, was assessed. In blossoms, A. flavus frequencies ranged from 0 to 4.5%, depending on the year of study. Frequencies of honey bees carrying A. flavus ranged from 6.5 to 10%. Only one A. flavus isolate recovered from a blossom in 2016 belonged to YV36, while members of the VCG were not detected contaminating honey bees. Exposure of pollinator honey bees to AF36 was detected to be very low. The density of several Aspergillus species was found to increase during almond hull split and throughout the final stages of maturation; this also occurred in pistachio orchards during the maturation period. Additionally, we found that AF36 effectively limited almond aflatoxin contamination in laboratory assays. This study provides knowledge and understanding of the seasonal dynamics of Aspergillus fungi and will help design aflatoxin management strategies for almond. The evidence of the low levels of VCG YV36 encountered on almond blossoms and bees during pollination and AF36’s effectiveness in limiting aflatoxin contamination in almond provided additional support for the registration of AF36 with USEPA to use in almond in California. |
| format | Journal Article |
| id | CGSpace96134 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace961342025-11-11T10:14:13Z Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California Ortega Beltran, A. Moral, J. Puckett, R.D. Morgan, D.P. Cotty, P.J. Michailides, T. almonds aflatoxins honey bees aspergillus flavus california Interactions between pathogenic and nonpathogenic fungal species in the tree canopy are complex and can determine if disease will manifest in the plant and in other organisms such as honey bees. Seasonal dynamics of fungi were studied in an almond orchard in California where experimental release of the atoxigenic biopesticide Aspergillus flavus AF36 to displace toxigenic Aspergillus strains has been conducted for five years. The presence of the vegetative compatibility group (VCG) YV36, to which AF36 belongs, in the blossoms, and the honey bees that attend these blossoms, was assessed. In blossoms, A. flavus frequencies ranged from 0 to 4.5%, depending on the year of study. Frequencies of honey bees carrying A. flavus ranged from 6.5 to 10%. Only one A. flavus isolate recovered from a blossom in 2016 belonged to YV36, while members of the VCG were not detected contaminating honey bees. Exposure of pollinator honey bees to AF36 was detected to be very low. The density of several Aspergillus species was found to increase during almond hull split and throughout the final stages of maturation; this also occurred in pistachio orchards during the maturation period. Additionally, we found that AF36 effectively limited almond aflatoxin contamination in laboratory assays. This study provides knowledge and understanding of the seasonal dynamics of Aspergillus fungi and will help design aflatoxin management strategies for almond. The evidence of the low levels of VCG YV36 encountered on almond blossoms and bees during pollination and AF36’s effectiveness in limiting aflatoxin contamination in almond provided additional support for the registration of AF36 with USEPA to use in almond in California. 2018-06-20 2018-07-13T12:47:07Z 2018-07-13T12:47:07Z Journal Article https://hdl.handle.net/10568/96134 en Open Access application/pdf Public Library of Science Ortega-Beltran, A., Moral, J., Puckett, R.D., Morgan, D.P., Cotty, P.J., Michailides, T.J. (2018). Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California. PLoS ONE 13(6), 1-15 |
| spellingShingle | almonds aflatoxins honey bees aspergillus flavus california Ortega Beltran, A. Moral, J. Puckett, R.D. Morgan, D.P. Cotty, P.J. Michailides, T. Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California |
| title | Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California |
| title_full | Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California |
| title_fullStr | Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California |
| title_full_unstemmed | Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California |
| title_short | Fungal communities associated with almond throughout crop development: implications for aflatoxin biocontrol management in California |
| title_sort | fungal communities associated with almond throughout crop development implications for aflatoxin biocontrol management in california |
| topic | almonds aflatoxins honey bees aspergillus flavus california |
| url | https://hdl.handle.net/10568/96134 |
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