Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses
In vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this study...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/96123 |
| _version_ | 1855535212470992896 |
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| author | Bömer, M. Rathnayake, A.I. Visendi, P. Sewe, S.O. Sicat, J.P.A. Silva, G. Kumar, P. Lava Seal, S.E. |
| author_browse | Bömer, M. Kumar, P. Lava Rathnayake, A.I. Seal, S.E. Sewe, S.O. Sicat, J.P.A. Silva, G. Visendi, P. |
| author_facet | Bömer, M. Rathnayake, A.I. Visendi, P. Sewe, S.O. Sicat, J.P.A. Silva, G. Kumar, P. Lava Seal, S.E. |
| author_sort | Bömer, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this study, we evaluated next-generation sequencing (NGS) for virus detection following yam propagation using a robust tissue culture methodology. We detected and assembled the genomes of novel isolates of already characterised viral species of the genera Badnavirus and Potyvirus, confirming the utility of NGS in diagnosing yam viruses and contributing towards the safe distribution of germplasm. |
| format | Journal Article |
| id | CGSpace96123 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace961232025-11-11T10:41:28Z Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses Bömer, M. Rathnayake, A.I. Visendi, P. Sewe, S.O. Sicat, J.P.A. Silva, G. Kumar, P. Lava Seal, S.E. in vitro culture rna sequence dioscorea mosaic virus tissue culture yams virus free plants In vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this study, we evaluated next-generation sequencing (NGS) for virus detection following yam propagation using a robust tissue culture methodology. We detected and assembled the genomes of novel isolates of already characterised viral species of the genera Badnavirus and Potyvirus, confirming the utility of NGS in diagnosing yam viruses and contributing towards the safe distribution of germplasm. 2018-06-19 2018-07-12T16:09:14Z 2018-07-12T16:09:14Z Journal Article https://hdl.handle.net/10568/96123 en Open Access application/pdf Elsevier Bömer, M., Rathnayake, A.I., Visendi, P., Sewe, S.O., Sicat, J.P.A., Silva, G., ... & Seal, S.E. (2018). Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses. Physiological and Molecular Plant Pathology, 1-13. |
| spellingShingle | in vitro culture rna sequence dioscorea mosaic virus tissue culture yams virus free plants Bömer, M. Rathnayake, A.I. Visendi, P. Sewe, S.O. Sicat, J.P.A. Silva, G. Kumar, P. Lava Seal, S.E. Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses |
| title | Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses |
| title_full | Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses |
| title_fullStr | Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses |
| title_full_unstemmed | Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses |
| title_short | Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses |
| title_sort | tissue culture and next generation sequencing a combined approach for detecting yam dioscorea spp viruses |
| topic | in vitro culture rna sequence dioscorea mosaic virus tissue culture yams virus free plants |
| url | https://hdl.handle.net/10568/96123 |
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