Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa
Hybrids were obtained after in vitro germination of embryos from interploid crosses between triploid ‘French' plantain cultivars (Musa spp. AAB group). ‘Ntanga 2' and ‘Bobby Tannap' with diploid banana (Musa acuminata subsp. burmannicoides) ‘Calcutta 4'. Cross-pollinated bunches were harvested at fu...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1998
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/96001 |
| _version_ | 1855518370700460032 |
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| author | Osuji, J.O. Vuylsteke, D.R. Ortíz, R. |
| author_browse | Ortíz, R. Osuji, J.O. Vuylsteke, D.R. |
| author_facet | Osuji, J.O. Vuylsteke, D.R. Ortíz, R. |
| author_sort | Osuji, J.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Hybrids were obtained after in vitro germination of embryos from interploid crosses between triploid ‘French' plantain cultivars (Musa spp. AAB group). ‘Ntanga 2' and ‘Bobby Tannap' with diploid banana (Musa acuminata subsp. burmannicoides) ‘Calcutta 4'. Cross-pollinated bunches were harvested at full maturity and ripened with acetylene in a room for 4 days. Seeds were extracted from peeled ripe fruits by squashing. Embryos from the seeds were excised aseptically after 2 days and germinated in vitro. Seedlings were subsequently planted in early evaluation trials after acclimatizing in the greenhouse. Chromosome counts were carried out on root tips of mature and maiden suckers to determine ploidy levels using a modified squashing technique. Counts showed that two of the hybrids were aneuploids (trisomics) with somatic chromosome number of 2n = 2x + 1 = 23. One hybrid was diploid while the other two were tetraploids. Tetraploids are the most promising hybrids for the genetic improvement of plantains. Diploids are valuable material for further improvement of the plantain genome at this ploidy level. Trisomics provide means for further characterization of the Musa genome and physical gene mapping in plantain and banana. |
| format | Journal Article |
| id | CGSpace96001 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| record_format | dspace |
| spelling | CGSpace960012024-03-06T10:16:43Z Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa Osuji, J.O. Vuylsteke, D.R. Ortíz, R. aneuploids bananas breeding cytogenetics plantains ploidy manipulation Hybrids were obtained after in vitro germination of embryos from interploid crosses between triploid ‘French' plantain cultivars (Musa spp. AAB group). ‘Ntanga 2' and ‘Bobby Tannap' with diploid banana (Musa acuminata subsp. burmannicoides) ‘Calcutta 4'. Cross-pollinated bunches were harvested at full maturity and ripened with acetylene in a room for 4 days. Seeds were extracted from peeled ripe fruits by squashing. Embryos from the seeds were excised aseptically after 2 days and germinated in vitro. Seedlings were subsequently planted in early evaluation trials after acclimatizing in the greenhouse. Chromosome counts were carried out on root tips of mature and maiden suckers to determine ploidy levels using a modified squashing technique. Counts showed that two of the hybrids were aneuploids (trisomics) with somatic chromosome number of 2n = 2x + 1 = 23. One hybrid was diploid while the other two were tetraploids. Tetraploids are the most promising hybrids for the genetic improvement of plantains. Diploids are valuable material for further improvement of the plantain genome at this ploidy level. Trisomics provide means for further characterization of the Musa genome and physical gene mapping in plantain and banana. 1998 2018-07-05T06:30:25Z 2018-07-05T06:30:25Z Journal Article https://hdl.handle.net/10568/96001 en Limited Access Osuji, J.O., Vuylsteke, D. & Ortiz, R. (1998). Ploidy variation in hybrids from interploid 3x x 2x crosses in Musa. Tropicultura, 15(1), 37-39. |
| spellingShingle | aneuploids bananas breeding cytogenetics plantains ploidy manipulation Osuji, J.O. Vuylsteke, D.R. Ortíz, R. Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa |
| title | Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa |
| title_full | Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa |
| title_fullStr | Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa |
| title_full_unstemmed | Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa |
| title_short | Ploidy variation in hybrids from interploid 3x X 2x crosses in Musa |
| title_sort | ploidy variation in hybrids from interploid 3x x 2x crosses in musa |
| topic | aneuploids bananas breeding cytogenetics plantains ploidy manipulation |
| url | https://hdl.handle.net/10568/96001 |
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