Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.)

The response of white yam (Dioscorea rotundata Poir.) nodal cuttings to eight different carbon sources (sucrose, glucose, mannose, maltose, galactose, fructose, lactose, and sorbitol) at two concentrations (3 and 5%) in modified Murashige and Skoog medium was investigated. The number of nodes per pl...

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Main Authors: Ng, S.Y.C., Mantell, S.H.
Format: Journal Article
Language:Inglés
Published: 1998
Subjects:
Online Access:https://hdl.handle.net/10568/95996
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author Ng, S.Y.C.
Mantell, S.H.
author_browse Mantell, S.H.
Ng, S.Y.C.
author_facet Ng, S.Y.C.
Mantell, S.H.
author_sort Ng, S.Y.C.
collection Repository of Agricultural Research Outputs (CGSpace)
description The response of white yam (Dioscorea rotundata Poir.) nodal cuttings to eight different carbon sources (sucrose, glucose, mannose, maltose, galactose, fructose, lactose, and sorbitol) at two concentrations (3 and 5%) in modified Murashige and Skoog medium was investigated. The number of nodes per plant (NNP), plantlet fresh weight, number of leaves per plant (NLP), percentage tuberization, number of micro-tubers per plant (NTP), and micro-tuber weight per plant (TWP) were significantly different among the carbon sources (P < 0.01). There were no significant differences in terms of micro-tuber weight per tuber and plant dry matter content. In terms of NNP and NLP, fructose, glucose, mannose, and sucrose at 3 and 5%, and sorbitol at 5% were comparable. However, nodal cuttings were unable to produce tubers in media containing sorbitol. All the explants cultured in 5% sucrose, 5% fructose, and 3 and 5% mannose produced micro-tubers. The NTP ranged from 0.9 (3% mannose) to 1.8 (5% sucrose), while 5% glucose gave the highest TWP, followed by 5% sucrose. The best carbon sources for in vitro tuberization were sucrose and fructose.
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spelling CGSpace959962023-02-15T06:49:30Z Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.) Ng, S.Y.C. Mantell, S.H. white yam carbon source nodal cuttings micro-tuberization micro-tuber The response of white yam (Dioscorea rotundata Poir.) nodal cuttings to eight different carbon sources (sucrose, glucose, mannose, maltose, galactose, fructose, lactose, and sorbitol) at two concentrations (3 and 5%) in modified Murashige and Skoog medium was investigated. The number of nodes per plant (NNP), plantlet fresh weight, number of leaves per plant (NLP), percentage tuberization, number of micro-tubers per plant (NTP), and micro-tuber weight per plant (TWP) were significantly different among the carbon sources (P < 0.01). There were no significant differences in terms of micro-tuber weight per tuber and plant dry matter content. In terms of NNP and NLP, fructose, glucose, mannose, and sucrose at 3 and 5%, and sorbitol at 5% were comparable. However, nodal cuttings were unable to produce tubers in media containing sorbitol. All the explants cultured in 5% sucrose, 5% fructose, and 3 and 5% mannose produced micro-tubers. The NTP ranged from 0.9 (3% mannose) to 1.8 (5% sucrose), while 5% glucose gave the highest TWP, followed by 5% sucrose. The best carbon sources for in vitro tuberization were sucrose and fructose. 1998 2018-07-05T06:30:24Z 2018-07-05T06:30:24Z Journal Article https://hdl.handle.net/10568/95996 en Limited Access Ng, S.Y.C. & Mantell, S.H. (1998). Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.). Tropical Agriculture, 75, 243-247.
spellingShingle white yam
carbon source
nodal cuttings
micro-tuberization
micro-tuber
Ng, S.Y.C.
Mantell, S.H.
Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.)
title Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.)
title_full Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.)
title_fullStr Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.)
title_full_unstemmed Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.)
title_short Influence of carbon source on in vitro tuberization and growth of white yam (Dioscorea rotundata Poir.)
title_sort influence of carbon source on in vitro tuberization and growth of white yam dioscorea rotundata poir
topic white yam
carbon source
nodal cuttings
micro-tuberization
micro-tuber
url https://hdl.handle.net/10568/95996
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