Banana die-back virus- a new virus infecting banana in Nigeria
Two viruses naturally infect Musa in Nigeria: banana streak badnavirus (BSV) and cucumber mosaic cucumovirus (CMV). During a recent field survey at Ibadan (Nigeria), some severely stunted banana plants (cv. Valery) were found that tested negative for CMV, banana bunchy-top virus, and BSV. The plants...
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| Format: | Journal Article |
| Language: | Inglés |
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Scientific Societies
1998
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/95955 |
| _version_ | 1855532254654103552 |
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| author | Hughes, J. Speijer, P. Olatunde, O. |
| author_browse | Hughes, J. Olatunde, O. Speijer, P. |
| author_facet | Hughes, J. Speijer, P. Olatunde, O. |
| author_sort | Hughes, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Two viruses naturally infect Musa in Nigeria: banana streak badnavirus (BSV) and cucumber mosaic cucumovirus (CMV). During a recent field survey at Ibadan (Nigeria), some severely stunted banana plants (cv. Valery) were found that tested negative for CMV, banana bunchy-top virus, and BSV. The plants had symptoms of leaf crinkling, leaf necrosis, and cigar-leaf die-back. Subsequent suckers from the same mats were progressively more stunted. A 28- to 30-nm isometric virus was purified, and used for the production of antibodies, from the affected plants with (NH4)2SO4 to precipitate the virus. The antiserum (titer of 1:10,000) was used in enzyme-linked immunosorbent assay and immunosorbent electron microscopy to detect the virus. Mechanical inoculation with partially purified virus preparations resulted in stunting and development of pinpoint chlorotic lesions on Vigna unguiculata TVu-76 and symptomless systemic infection of Nicotiana occidentalis. The virus was not mechanically transmissible from N. occidentalis to banana. A serological relationship between this virus, banana die-back virus (BDBV), and tobacco ringspot, tomato ringspot, and cacao necrosis nepoviruses was found. The nematode species around the affected banana plants were isolated: Helicotylenchus multicinctus (Cobb) Golden was the dominant species, low numbers of H. dihystera (Cobb) Sher were present, but no virustransmitting nematodes were found in soil or banana roots. Further studies are needed to determine the mode of spread of BDBV, the implications for banana/plantain production in sub-Saharan Africa, and the safe international movement of germplasm. |
| format | Journal Article |
| id | CGSpace95955 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace959552024-05-15T05:11:55Z Banana die-back virus- a new virus infecting banana in Nigeria Hughes, J. Speijer, P. Olatunde, O. banana streak badnavirus cucumber mosaic cucumovirus virus immunosorbent nematodes Two viruses naturally infect Musa in Nigeria: banana streak badnavirus (BSV) and cucumber mosaic cucumovirus (CMV). During a recent field survey at Ibadan (Nigeria), some severely stunted banana plants (cv. Valery) were found that tested negative for CMV, banana bunchy-top virus, and BSV. The plants had symptoms of leaf crinkling, leaf necrosis, and cigar-leaf die-back. Subsequent suckers from the same mats were progressively more stunted. A 28- to 30-nm isometric virus was purified, and used for the production of antibodies, from the affected plants with (NH4)2SO4 to precipitate the virus. The antiserum (titer of 1:10,000) was used in enzyme-linked immunosorbent assay and immunosorbent electron microscopy to detect the virus. Mechanical inoculation with partially purified virus preparations resulted in stunting and development of pinpoint chlorotic lesions on Vigna unguiculata TVu-76 and symptomless systemic infection of Nicotiana occidentalis. The virus was not mechanically transmissible from N. occidentalis to banana. A serological relationship between this virus, banana die-back virus (BDBV), and tobacco ringspot, tomato ringspot, and cacao necrosis nepoviruses was found. The nematode species around the affected banana plants were isolated: Helicotylenchus multicinctus (Cobb) Golden was the dominant species, low numbers of H. dihystera (Cobb) Sher were present, but no virustransmitting nematodes were found in soil or banana roots. Further studies are needed to determine the mode of spread of BDBV, the implications for banana/plantain production in sub-Saharan Africa, and the safe international movement of germplasm. 1998-01 2018-07-05T06:30:18Z 2018-07-05T06:30:18Z Journal Article https://hdl.handle.net/10568/95955 en Limited Access Scientific Societies Hughes, J., Speijer, P. & Olatunde, O. (1998). Banana dieback virus a new virus infecting banana in Nigeria. Plant Disease, 82(1), 129-129. |
| spellingShingle | banana streak badnavirus cucumber mosaic cucumovirus virus immunosorbent nematodes Hughes, J. Speijer, P. Olatunde, O. Banana die-back virus- a new virus infecting banana in Nigeria |
| title | Banana die-back virus- a new virus infecting banana in Nigeria |
| title_full | Banana die-back virus- a new virus infecting banana in Nigeria |
| title_fullStr | Banana die-back virus- a new virus infecting banana in Nigeria |
| title_full_unstemmed | Banana die-back virus- a new virus infecting banana in Nigeria |
| title_short | Banana die-back virus- a new virus infecting banana in Nigeria |
| title_sort | banana die back virus a new virus infecting banana in nigeria |
| topic | banana streak badnavirus cucumber mosaic cucumovirus virus immunosorbent nematodes |
| url | https://hdl.handle.net/10568/95955 |
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