Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population

In a study to map out the genes confering resistanceto African cassava msaic disease (ACMD), a cost-effective protocol for zygotic embryo culture developed at the International Institute of Tropical Agriculture(IITA) using 1/2 strenght Murashige and Skoog's basal media, was employed to germinatethe...

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Main Authors: Akano, A.O., Ng, S.Y.C., Dixon, A., Thottappilly, G.
Format: Journal Article
Language:Inglés
Published: 1998
Subjects:
Online Access:https://hdl.handle.net/10568/95954
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author Akano, A.O.
Ng, S.Y.C.
Dixon, A.
Thottappilly, G.
author_browse Akano, A.O.
Dixon, A.
Ng, S.Y.C.
Thottappilly, G.
author_facet Akano, A.O.
Ng, S.Y.C.
Dixon, A.
Thottappilly, G.
author_sort Akano, A.O.
collection Repository of Agricultural Research Outputs (CGSpace)
description In a study to map out the genes confering resistanceto African cassava msaic disease (ACMD), a cost-effective protocol for zygotic embryo culture developed at the International Institute of Tropical Agriculture(IITA) using 1/2 strenght Murashige and Skoog's basal media, was employed to germinatethe F1 populations. The F1 crosses wee generated from cross combinations between ACMD resistant and susceptible and local cassava germplasm. These crosses were TMS 30572 x TME 117, TMS 30572 x TME 4, and TMS 30555 x TME 3, giving three mapping populations. Four weeks after explanting embryo axes, progenies of the F1 cross of TMS 30555 x TME 3 perfomed best with 88% of the explanted embryos poducing two or more nodes, followed by those of the F1 cross TMS 30572 x TME 4, with 79%, and TMS 30572 x TME 117, germilings had 4 to 5 nodes, and 3, 5, and 9% in F1 crosses of TMS 30572 x TME 117, TMS 30572 x TME 4, and TMS 30555 X tme 3, respectively.
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spelling CGSpace959542023-02-15T06:49:26Z Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population Akano, A.O. Ng, S.Y.C. Dixon, A. Thottappilly, G. african cassava mosaic virus zygotic embryo embryo axes mapping population In a study to map out the genes confering resistanceto African cassava msaic disease (ACMD), a cost-effective protocol for zygotic embryo culture developed at the International Institute of Tropical Agriculture(IITA) using 1/2 strenght Murashige and Skoog's basal media, was employed to germinatethe F1 populations. The F1 crosses wee generated from cross combinations between ACMD resistant and susceptible and local cassava germplasm. These crosses were TMS 30572 x TME 117, TMS 30572 x TME 4, and TMS 30555 x TME 3, giving three mapping populations. Four weeks after explanting embryo axes, progenies of the F1 cross of TMS 30555 x TME 3 perfomed best with 88% of the explanted embryos poducing two or more nodes, followed by those of the F1 cross TMS 30572 x TME 4, with 79%, and TMS 30572 x TME 117, germilings had 4 to 5 nodes, and 3, 5, and 9% in F1 crosses of TMS 30572 x TME 117, TMS 30572 x TME 4, and TMS 30555 X tme 3, respectively. 1998 2018-07-05T06:30:18Z 2018-07-05T06:30:18Z Journal Article https://hdl.handle.net/10568/95954 en Limited Access Akano, A.O., Ng, S.Y.C., Dixon, A. & Thottappilly, G. (1998). Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population. Tropical Agriculture, 75, 217-219.
spellingShingle african cassava mosaic virus
zygotic embryo
embryo axes
mapping population
Akano, A.O.
Ng, S.Y.C.
Dixon, A.
Thottappilly, G.
Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population
title Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population
title_full Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population
title_fullStr Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population
title_full_unstemmed Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population
title_short Application of embryo culture in germinating African cassava mosaic disease resistant gene mapping population
title_sort application of embryo culture in germinating african cassava mosaic disease resistant gene mapping population
topic african cassava mosaic virus
zygotic embryo
embryo axes
mapping population
url https://hdl.handle.net/10568/95954
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