Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants
Pseudocercospora fijiensis, causal agent of the black Sigatoka disease (BSD) of Musa spp., has spread globally since its discovery in Fiji 1963 to all the banana and plantain growing areas across the globe. It is becoming the most damaging and economically important disease of this crop. The identif...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/92994 |
| _version_ | 1855531660669353984 |
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| author | Onyilo, F. Tusiime, Geoffrey Tripathi, J.N. Chen, L.H. Falk, B. Stergiopoulos, I. Tushemereirwe, Wilberforce K. Kubiriba, Jerome Tripathi, L. |
| author_browse | Chen, L.H. Falk, B. Kubiriba, Jerome Onyilo, F. Stergiopoulos, I. Tripathi, J.N. Tripathi, L. Tushemereirwe, Wilberforce K. Tusiime, Geoffrey |
| author_facet | Onyilo, F. Tusiime, Geoffrey Tripathi, J.N. Chen, L.H. Falk, B. Stergiopoulos, I. Tushemereirwe, Wilberforce K. Kubiriba, Jerome Tripathi, L. |
| author_sort | Onyilo, F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pseudocercospora fijiensis, causal agent of the black Sigatoka disease (BSD) of Musa spp., has spread globally since its discovery in Fiji 1963 to all the banana and plantain growing areas across the globe. It is becoming the most damaging and economically important disease of this crop. The identification and characterization of genes that regulate infection processes and pathogenicity in P. fijiensis will provide important knowledge for the development of disease-resistant cultivars. In many fungal plant pathogens, the Fus3 and Slt2 are reported to be essential for pathogenicity. Fus3 regulates filamentous-invasion pathways including the formation of infection structures, sporulation, virulence, and invasive and filamentous growth, whereas Slt2 is involved in the cell-wall integrity pathway, virulence, invasive growth, and colonization in host tissues. Here, we used RNAi-mediated gene silencing to investigate the role of the Slt2 and Fus3 homologs in P. fijiensis in pathogen invasiveness, growth and pathogenicity. The PfSlt2 and PfFus3 silenced P. fijiensis transformants showed significantly lower gene expression and reduced virulence, invasive growth, and lower biomass in infected leaf tissues of East African Highland Banana (EAHB). This study suggests that Slt2 and Fus3 MAPK signaling pathways play important roles in plant infection and pathogenic growth of fungal pathogens. The silencing of these vital fungal genes through host-induced gene silencing (HIG) could be an alternative strategy for developing transgenic banana and plantain resistant to BSD. |
| format | Journal Article |
| id | CGSpace92994 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace929942025-11-11T10:36:48Z Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants Onyilo, F. Tusiime, Geoffrey Tripathi, J.N. Chen, L.H. Falk, B. Stergiopoulos, I. Tushemereirwe, Wilberforce K. Kubiriba, Jerome Tripathi, L. pseudocercospora protein kinase pathogenicity black sigatoka disease plantains bananas host plant Pseudocercospora fijiensis, causal agent of the black Sigatoka disease (BSD) of Musa spp., has spread globally since its discovery in Fiji 1963 to all the banana and plantain growing areas across the globe. It is becoming the most damaging and economically important disease of this crop. The identification and characterization of genes that regulate infection processes and pathogenicity in P. fijiensis will provide important knowledge for the development of disease-resistant cultivars. In many fungal plant pathogens, the Fus3 and Slt2 are reported to be essential for pathogenicity. Fus3 regulates filamentous-invasion pathways including the formation of infection structures, sporulation, virulence, and invasive and filamentous growth, whereas Slt2 is involved in the cell-wall integrity pathway, virulence, invasive growth, and colonization in host tissues. Here, we used RNAi-mediated gene silencing to investigate the role of the Slt2 and Fus3 homologs in P. fijiensis in pathogen invasiveness, growth and pathogenicity. The PfSlt2 and PfFus3 silenced P. fijiensis transformants showed significantly lower gene expression and reduced virulence, invasive growth, and lower biomass in infected leaf tissues of East African Highland Banana (EAHB). This study suggests that Slt2 and Fus3 MAPK signaling pathways play important roles in plant infection and pathogenic growth of fungal pathogens. The silencing of these vital fungal genes through host-induced gene silencing (HIG) could be an alternative strategy for developing transgenic banana and plantain resistant to BSD. 2018-03-13 2018-06-01T11:25:31Z 2018-06-01T11:25:31Z Journal Article https://hdl.handle.net/10568/92994 en Open Access application/pdf Frontiers Media Onyilo, F., Tusiime, G., Tripathi, J.N., Chen, L.H., Falk, B., Stergiopoulos, I., ... & Tripathi, L. (2018). Silencing of the mitogen activated protein kinases (MAPK) fus3 and slt2 in Pseudocercospora fijiensis reduces growth and virulence on host plants. Frontiers in Plant Science, 9(291), 1-12. |
| spellingShingle | pseudocercospora protein kinase pathogenicity black sigatoka disease plantains bananas host plant Onyilo, F. Tusiime, Geoffrey Tripathi, J.N. Chen, L.H. Falk, B. Stergiopoulos, I. Tushemereirwe, Wilberforce K. Kubiriba, Jerome Tripathi, L. Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants |
| title | Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants |
| title_full | Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants |
| title_fullStr | Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants |
| title_full_unstemmed | Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants |
| title_short | Silencing of the mitogen-activated protein kinases (MAPK) fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants |
| title_sort | silencing of the mitogen activated protein kinases mapk fus3 and slt2 in pseudocercospora fijiensis reduces growth and virulence on host plants |
| topic | pseudocercospora protein kinase pathogenicity black sigatoka disease plantains bananas host plant |
| url | https://hdl.handle.net/10568/92994 |
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