Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes
Late blight, caused by the oomycete Phytophthora infestans, is a threat to potato cropping systems worldwide. In the Ecuadorian Andes, despite a high late blight incidence in foliage, tuber blight is rare. In this work, the hypothesis that Ecuadorian Andean soils are naturally suppressive to P. infe...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/92947 |
| _version_ | 1855525236463632384 |
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| author | Orquera-Tornakian, G. Díaz, C.I. Mogrovejo, D.C. Villamarin, D.J. Jarrin, F. Ponce, L.K. Oliva, R. Gia, J. Forbes, G. Flores, F.J. Garzon, C.D. Molineros, J. Koch, A. Benitez, M.S. Andrade-Piedra, J.L. |
| author_browse | Andrade-Piedra, J.L. Benitez, M.S. Díaz, C.I. Flores, F.J. Forbes, G. Garzon, C.D. Gia, J. Jarrin, F. Koch, A. Mogrovejo, D.C. Molineros, J. Oliva, R. Orquera-Tornakian, G. Ponce, L.K. Villamarin, D.J. |
| author_facet | Orquera-Tornakian, G. Díaz, C.I. Mogrovejo, D.C. Villamarin, D.J. Jarrin, F. Ponce, L.K. Oliva, R. Gia, J. Forbes, G. Flores, F.J. Garzon, C.D. Molineros, J. Koch, A. Benitez, M.S. Andrade-Piedra, J.L. |
| author_sort | Orquera-Tornakian, G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Late blight, caused by the oomycete Phytophthora infestans, is a threat to potato cropping systems worldwide. In the Ecuadorian Andes, despite a high late blight incidence in foliage, tuber blight is rare. In this work, the hypothesis that Ecuadorian Andean soils are naturally suppressive to P. infestans tuber infection was evaluated. Soils from four potato growing regions were assessed for disease suppressiveness by determining the effects of soil heat treatment on P. infestans sporangia and their ability to infect potato slices after 1, 8, 15 and 30 days of exposure to soils. Tuber infection after inoculation with P. infestans infested soils was consistently lower during the evaluation period compared with heat treated soils. Fresh, untreated soils affected germination and viability of P. infestans sporangia in a site dependent manner. In addition, the effect of heat treatment on soil bacterial communities was assessed through terminal restriction fragment length polymorphism analysis of the 16S rDNA gene region. Heat treatment disrupted bacterial community composition, and a subset of terminal restriction fragments (TRF) was either positively or negatively correlated with tuber infection. Bacterial TRF negatively correlated with tuber infection corresponded in fragment size to taxa with known ability to inhibit pathogens and promote plant growth. Finally, bacterial isolates obtained from untreated soils, which inhibited P. infestans growth in vitro, represented 22–47% of isolates recovered, and matched classes predicted by the TRFs. This work represents a first step in understanding the mechanisms behind. |
| format | Journal Article |
| id | CGSpace92947 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace929472024-11-14T08:04:30Z Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes Orquera-Tornakian, G. Díaz, C.I. Mogrovejo, D.C. Villamarin, D.J. Jarrin, F. Ponce, L.K. Oliva, R. Gia, J. Forbes, G. Flores, F.J. Garzon, C.D. Molineros, J. Koch, A. Benitez, M.S. Andrade-Piedra, J.L. potatoes phytophthora infestans soil polimorphism Late blight, caused by the oomycete Phytophthora infestans, is a threat to potato cropping systems worldwide. In the Ecuadorian Andes, despite a high late blight incidence in foliage, tuber blight is rare. In this work, the hypothesis that Ecuadorian Andean soils are naturally suppressive to P. infestans tuber infection was evaluated. Soils from four potato growing regions were assessed for disease suppressiveness by determining the effects of soil heat treatment on P. infestans sporangia and their ability to infect potato slices after 1, 8, 15 and 30 days of exposure to soils. Tuber infection after inoculation with P. infestans infested soils was consistently lower during the evaluation period compared with heat treated soils. Fresh, untreated soils affected germination and viability of P. infestans sporangia in a site dependent manner. In addition, the effect of heat treatment on soil bacterial communities was assessed through terminal restriction fragment length polymorphism analysis of the 16S rDNA gene region. Heat treatment disrupted bacterial community composition, and a subset of terminal restriction fragments (TRF) was either positively or negatively correlated with tuber infection. Bacterial TRF negatively correlated with tuber infection corresponded in fragment size to taxa with known ability to inhibit pathogens and promote plant growth. Finally, bacterial isolates obtained from untreated soils, which inhibited P. infestans growth in vitro, represented 22–47% of isolates recovered, and matched classes predicted by the TRFs. This work represents a first step in understanding the mechanisms behind. 2018-08-03 2018-05-29T16:20:47Z 2018-05-29T16:20:47Z Journal Article https://hdl.handle.net/10568/92947 en Limited Access Wiley Orquera-Tornakian, G.; Diaz, C.I.; Mogrovejo, D.C.; Villamarin, D.J.; Jarrin, F.; Ponce, L.K.; Oliva, R.; Gia, J.; Forbes, G.A.; Andrade-Piedra, J.; Flores, F.J.; Garzon, C.D.; Molineros, J.; Koch, A.; Benitez, M.S. 2018. Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes. Plant Pathology. (UK). ISSN 0032-0862. 67:7. pp. 1562-1573. |
| spellingShingle | potatoes phytophthora infestans soil polimorphism Orquera-Tornakian, G. Díaz, C.I. Mogrovejo, D.C. Villamarin, D.J. Jarrin, F. Ponce, L.K. Oliva, R. Gia, J. Forbes, G. Flores, F.J. Garzon, C.D. Molineros, J. Koch, A. Benitez, M.S. Andrade-Piedra, J.L. Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes |
| title | Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes |
| title_full | Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes |
| title_fullStr | Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes |
| title_full_unstemmed | Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes |
| title_short | Characterization of tuber blight suppressive soils from four provinces of the Ecuadorean Andes |
| title_sort | characterization of tuber blight suppressive soils from four provinces of the ecuadorean andes |
| topic | potatoes phytophthora infestans soil polimorphism |
| url | https://hdl.handle.net/10568/92947 |
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