R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1
The Avr avirulence gene of Phytophthora infestans and R gene of the potato are the genetic components of the gene-for-gene interaction resulting in host plant resistance. This effector-triggered immunity has been recently exploited to generate extreme resistance to late blight in potato by genetic e...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/92940 |
| _version_ | 1855524127438274560 |
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| author | Roman, M.L. Izarra, M. Lindqvist-Kreuze, H. Rivera, C. Gamboa, S. Tovar, J.C. Forbes, G.A. Kreuze, Jan F. Ghislain, M. |
| author_browse | Forbes, G.A. Gamboa, S. Ghislain, M. Izarra, M. Kreuze, Jan F. Lindqvist-Kreuze, H. Rivera, C. Roman, M.L. Tovar, J.C. |
| author_facet | Roman, M.L. Izarra, M. Lindqvist-Kreuze, H. Rivera, C. Gamboa, S. Tovar, J.C. Forbes, G.A. Kreuze, Jan F. Ghislain, M. |
| author_sort | Roman, M.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The Avr avirulence gene of Phytophthora infestans and R gene of the potato are the genetic components of the gene-for-gene interaction resulting in host plant resistance. This effector-triggered immunity has been recently exploited to generate extreme resistance to late blight in potato by genetic engineering. The choice of the R genes, their number forming a R gene stack, and the pathogen Avr gene diversity will likely determine how long this extreme resistance will last. Here, we report on a comparative study on the Rpi-vnt1.1 gene which originated from Solanum venturii, and was introduced in the potato variety ‘Desiree’ by genetic transformation, and the Avr-vnt1 gene from two isolates of the EC-1 lineage of P. infestans. EC-1 was reported previously as not expressing the Avr-vnt1 gene and, therefore, being virulent on Rpi-vnt1.1 transgenic plants. Unexpectedly, whole-plant resistance assays identified 5 out of 52 transgenic events as resistant to two isolates of P. infestans, POX067 and POX109, belonging to the EC-1 lineage. We demonstrated that in both isolates, the Avr-vnt1.1 gene was expressed at a low level. Expression of the Rpi-vnt1.1 gene was shown to be rapidly increased by two-fold and subsequently to have steady state expression for at least 5 days after the inoculation. The Rpi-vnt1.1 gene in addition to other R genes as a stack in farmers’ preferred varieties will confer extreme resistance to late blight disease and rotations of plants with different R-gene-stack in time is likely to last longer than plants with single R gene. |
| format | Journal Article |
| id | CGSpace92940 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace929402024-05-01T08:17:07Z R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1 Roman, M.L. Izarra, M. Lindqvist-Kreuze, H. Rivera, C. Gamboa, S. Tovar, J.C. Forbes, G.A. Kreuze, Jan F. Ghislain, M. potatoes phytophthora infestans disease resistance transgenics The Avr avirulence gene of Phytophthora infestans and R gene of the potato are the genetic components of the gene-for-gene interaction resulting in host plant resistance. This effector-triggered immunity has been recently exploited to generate extreme resistance to late blight in potato by genetic engineering. The choice of the R genes, their number forming a R gene stack, and the pathogen Avr gene diversity will likely determine how long this extreme resistance will last. Here, we report on a comparative study on the Rpi-vnt1.1 gene which originated from Solanum venturii, and was introduced in the potato variety ‘Desiree’ by genetic transformation, and the Avr-vnt1 gene from two isolates of the EC-1 lineage of P. infestans. EC-1 was reported previously as not expressing the Avr-vnt1 gene and, therefore, being virulent on Rpi-vnt1.1 transgenic plants. Unexpectedly, whole-plant resistance assays identified 5 out of 52 transgenic events as resistant to two isolates of P. infestans, POX067 and POX109, belonging to the EC-1 lineage. We demonstrated that in both isolates, the Avr-vnt1.1 gene was expressed at a low level. Expression of the Rpi-vnt1.1 gene was shown to be rapidly increased by two-fold and subsequently to have steady state expression for at least 5 days after the inoculation. The Rpi-vnt1.1 gene in addition to other R genes as a stack in farmers’ preferred varieties will confer extreme resistance to late blight disease and rotations of plants with different R-gene-stack in time is likely to last longer than plants with single R gene. 2017-11 2018-05-28T19:20:01Z 2018-05-28T19:20:01Z Journal Article https://hdl.handle.net/10568/92940 en Open Access Springer Roman, M.L.; Izarra, M.; Lindqvist-Kreuze, H.; Rivera, C.; Gamboa, S.; Tovar, J.C.; Forbes, G.A.; Kreuze, J.; Ghislain, M. 2017. R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1. Plant Cell, Tissue and Organ Culture. (Netherlands). ISSN 0167-6857. 131(2):259-268. |
| spellingShingle | potatoes phytophthora infestans disease resistance transgenics Roman, M.L. Izarra, M. Lindqvist-Kreuze, H. Rivera, C. Gamboa, S. Tovar, J.C. Forbes, G.A. Kreuze, Jan F. Ghislain, M. R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1 |
| title | R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1 |
| title_full | R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1 |
| title_fullStr | R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1 |
| title_full_unstemmed | R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1 |
| title_short | R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1 |
| title_sort | r avr gene expression study of rpi vnt1 1 transgenic potato resistant to the phytophthora infestans clonal lineage ec 1 |
| topic | potatoes phytophthora infestans disease resistance transgenics |
| url | https://hdl.handle.net/10568/92940 |
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