A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda
During the 1990s, an epidemic of cassava mosaic virus disease caused major losses to cassava production in Uganda. Two factors associated with the epidemic were the occurrence of a novel recombinant begomovirus, EACMV-Ug, and unusually high populations of the whitefly vector, Bemisia tabaci. Here we...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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2002
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| Online Access: | https://hdl.handle.net/10568/92725 |
| _version_ | 1855518916351098880 |
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| author | Legg, James P. French, R. Rogan, D. Okao‐Okuja, G. Brown, J. |
| author_browse | Brown, J. French, R. Legg, James P. Okao‐Okuja, G. Rogan, D. |
| author_facet | Legg, James P. French, R. Rogan, D. Okao‐Okuja, G. Brown, J. |
| author_sort | Legg, James P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | During the 1990s, an epidemic of cassava mosaic virus disease caused major losses to cassava production in Uganda. Two factors associated with the epidemic were the occurrence of a novel recombinant begomovirus, EACMV-Ug, and unusually high populations of the whitefly vector, Bemisia tabaci. Here we present molecular evidence for the occurrence of two cassava-colonizing B. tabaci genotype clusters, Ug1 and Ug2, one of which, Ug2, can be consistently associated with the CMD epidemic in Uganda at the time of collection in 1997. By contrast, a second genotype cluster, Ug1, only occurred 'at' or 'ahead of' the epidemic 'front', sometimes in mixtures with Ug2. Comparison of mitochondrial cytochrome oxidase I gene sequences for Ug1 and Ug2 and well-studied B. tabaci reference populations indicated that the two Ugandan populations exhibited approximately 8% divergence, suggesting they represent distinct sub-Saharan African lineages. Neither Ugandan genotype cluster was identified as the widely distributed, polyphagous, and highly fecund B biotype of Old World origin, with which they both diverged by approximately 8%. Within genotype cluster divergence of Ug1 at 0.61 +/- 0.1% was twice that of Ug2 at 0.35 +/- 0.1%. Mismatch analysis suggested that Ug2 has undergone a recent population expansion and may be of nonUgandan origin, whereas Ug1 has diverged more slowly, and is likely to be an indigenous genotype cluster. |
| format | Journal Article |
| id | CGSpace92725 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| record_format | dspace |
| spelling | CGSpace927252023-02-15T06:46:58Z A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda Legg, James P. French, R. Rogan, D. Okao‐Okuja, G. Brown, J. cassava mosaic geminiviruses cmd eacmv-ug molecular markers whiteflies During the 1990s, an epidemic of cassava mosaic virus disease caused major losses to cassava production in Uganda. Two factors associated with the epidemic were the occurrence of a novel recombinant begomovirus, EACMV-Ug, and unusually high populations of the whitefly vector, Bemisia tabaci. Here we present molecular evidence for the occurrence of two cassava-colonizing B. tabaci genotype clusters, Ug1 and Ug2, one of which, Ug2, can be consistently associated with the CMD epidemic in Uganda at the time of collection in 1997. By contrast, a second genotype cluster, Ug1, only occurred 'at' or 'ahead of' the epidemic 'front', sometimes in mixtures with Ug2. Comparison of mitochondrial cytochrome oxidase I gene sequences for Ug1 and Ug2 and well-studied B. tabaci reference populations indicated that the two Ugandan populations exhibited approximately 8% divergence, suggesting they represent distinct sub-Saharan African lineages. Neither Ugandan genotype cluster was identified as the widely distributed, polyphagous, and highly fecund B biotype of Old World origin, with which they both diverged by approximately 8%. Within genotype cluster divergence of Ug1 at 0.61 +/- 0.1% was twice that of Ug2 at 0.35 +/- 0.1%. Mismatch analysis suggested that Ug2 has undergone a recent population expansion and may be of nonUgandan origin, whereas Ug1 has diverged more slowly, and is likely to be an indigenous genotype cluster. 2002 2018-05-17T09:03:17Z 2018-05-17T09:03:17Z Journal Article https://hdl.handle.net/10568/92725 en Limited Access Legg, J., French, R., Rogan, D., Okao‐Okuja, G. & Brown, J. (2002). A distinct Bemisia tabaci (Gennadius)(Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda. Molecular Ecology, 11(7), 1219-1229. |
| spellingShingle | cassava mosaic geminiviruses cmd eacmv-ug molecular markers whiteflies Legg, James P. French, R. Rogan, D. Okao‐Okuja, G. Brown, J. A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda |
| title | A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda |
| title_full | A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda |
| title_fullStr | A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda |
| title_full_unstemmed | A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda |
| title_short | A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda |
| title_sort | distinct bemisia tabaci gennadius hemiptera sternorrhyncha aleyrodidae genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in uganda |
| topic | cassava mosaic geminiviruses cmd eacmv-ug molecular markers whiteflies |
| url | https://hdl.handle.net/10568/92725 |
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