Identification of some Benin Republics Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA
DNA from twenty-three late maturing cultivars of Guinea yams (D. cayenensis/D. rotundata complex) from the Benin Republic that could not be separated using isozyme markers, were examined using randomly amplified polymorphic DNA (RAPD) markers with decamer primers of arbitrary sequence. All the twelv...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2000
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/92617 |
| _version_ | 1855531953283923968 |
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| author | Dansi, A. Mignouna, H. Zoundjihékpon, J. Sangare, A. Ahoussou, N. Asiedu, Robert |
| author_browse | Ahoussou, N. Asiedu, Robert Dansi, A. Mignouna, H. Sangare, A. Zoundjihékpon, J. |
| author_facet | Dansi, A. Mignouna, H. Zoundjihékpon, J. Sangare, A. Ahoussou, N. Asiedu, Robert |
| author_sort | Dansi, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | DNA from twenty-three late maturing cultivars of Guinea yams (D. cayenensis/D. rotundata complex) from the Benin Republic that could not be separated using isozyme markers, were examined using randomly amplified polymorphic DNA (RAPD) markers with decamer primers of arbitrary sequence. All the twelve primers tested were informative and yielded 63 amplified DNA bands from which 47 (75%) were polymorphic. Although no single primer produced polymorphic bands in all cultivars, the great majority of the cultivars were separated with the combinations of polymorphic bands generated by various primers. Putative duplicates and cultivar misclassifications were identified. Many morphologically distinct cultivars were close. The dwarf cultivar Tam-Sam considered as derived from Tabane, appeared more distant from the latter than was believed. RAPD analysis was found as a practical tool for the identification of duplicates toward establishment of an accurate core collection of Guinea yams in Benin Republic and in the other countries of the African yam belt. |
| format | Journal Article |
| id | CGSpace92617 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| record_format | dspace |
| spelling | CGSpace926172023-06-12T17:54:47Z Identification of some Benin Republics Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA Dansi, A. Mignouna, H. Zoundjihékpon, J. Sangare, A. Ahoussou, N. Asiedu, Robert core collection cultivar identification dioscorea cayenensis dioscorea rotundata complex guinea yam identification of duplicates rapd DNA from twenty-three late maturing cultivars of Guinea yams (D. cayenensis/D. rotundata complex) from the Benin Republic that could not be separated using isozyme markers, were examined using randomly amplified polymorphic DNA (RAPD) markers with decamer primers of arbitrary sequence. All the twelve primers tested were informative and yielded 63 amplified DNA bands from which 47 (75%) were polymorphic. Although no single primer produced polymorphic bands in all cultivars, the great majority of the cultivars were separated with the combinations of polymorphic bands generated by various primers. Putative duplicates and cultivar misclassifications were identified. Many morphologically distinct cultivars were close. The dwarf cultivar Tam-Sam considered as derived from Tabane, appeared more distant from the latter than was believed. RAPD analysis was found as a practical tool for the identification of duplicates toward establishment of an accurate core collection of Guinea yams in Benin Republic and in the other countries of the African yam belt. 2000 2018-05-17T09:02:52Z 2018-05-17T09:02:52Z Journal Article https://hdl.handle.net/10568/92617 en Limited Access Dansi, A., Mignouna, H., Zoundjihékpon, J., Sangare, A., Ahoussou, N. & Asiedu, R. (2000). Identification of some Benin Republic's Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using randomly amplified polymorphic DNA. Genetic Resources and Crop Evolution, 47, 619-625. |
| spellingShingle | core collection cultivar identification dioscorea cayenensis dioscorea rotundata complex guinea yam identification of duplicates rapd Dansi, A. Mignouna, H. Zoundjihékpon, J. Sangare, A. Ahoussou, N. Asiedu, Robert Identification of some Benin Republics Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA |
| title | Identification of some Benin Republics Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA |
| title_full | Identification of some Benin Republics Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA |
| title_fullStr | Identification of some Benin Republics Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA |
| title_full_unstemmed | Identification of some Benin Republics Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA |
| title_short | Identification of some Benin Republics Guinea yam (Dioscorea cayenensis/Dioscorea rotundata complex) cultivars using Randomly Amplified Polymorphic DNA |
| title_sort | identification of some benin republics guinea yam dioscorea cayenensis dioscorea rotundata complex cultivars using randomly amplified polymorphic dna |
| topic | core collection cultivar identification dioscorea cayenensis dioscorea rotundata complex guinea yam identification of duplicates rapd |
| url | https://hdl.handle.net/10568/92617 |
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