Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo
Objective: We aimed at determining the prevalence and characterizing the CaPV, determining the CaPV-PPRV coinfection prevalence and providing data about phylogenetic relationship between the fusion protein of PPRV and P32 gene of CaPV. Materials and methods: A total of 150 samples including animals...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
ScopeMed
2017
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| Acceso en línea: | https://hdl.handle.net/10568/92569 |
| _version_ | 1855529052194996224 |
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| author | Birindwa, B.A. Gitao, C.G. Bisimwa, P.N. Okafor, C. Bebora, C.L. |
| author_browse | Bebora, C.L. Birindwa, B.A. Bisimwa, P.N. Gitao, C.G. Okafor, C. |
| author_facet | Birindwa, B.A. Gitao, C.G. Bisimwa, P.N. Okafor, C. Bebora, C.L. |
| author_sort | Birindwa, B.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Objective: We aimed at determining the prevalence and characterizing the CaPV, determining the CaPV-PPRV coinfection prevalence and providing data about phylogenetic relationship between the fusion protein of PPRV and P32 gene of CaPV. Materials and methods: A total of 150 samples including animals swabs, tissues and blood were collected from unvaccinated goats in a PPR and/or Capripox outbreaks in South Kivu, Eastern of Democratic Republic of the Congo. Conventional PCR and reverse transcriptase (RT-PCR) were used respectively to amplify P32, RPO30, GPCR genes of Capripox virus and Fusion (F) protein of PPRV. Positive samples were sequenced for phylogenetic analysis. Results: Out of 150 tested animals, 64.7% (n=97/150) were PPRV positive, 52.7% (n=79/150) were Capripox positive and 38.7% (n=58/150) were positive for both PPRV and CaPV. The pairwise comparison of P32gene of CaPV and F gene of PPRV showed 99.75% of identity percentage among goatpox virus sequences, 96.95% among PPRV sequences and 47.91% between CaPV and PPRV sequences. Conclusion: The study has demonstrated high prevalence of CaP V-PPRV mixed infection in South Kivu. Lumpy skinvirus disease (LSVD) is a lineage circulating which has a genetic relationship between its P32gene and the Fgene of PPRV giving the challenge to differentiate the two diseases at the clinical farm level. |
| format | Journal Article |
| id | CGSpace92569 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | ScopeMed |
| publisherStr | ScopeMed |
| record_format | dspace |
| spelling | CGSpace925692025-11-11T10:32:52Z Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo Birindwa, B.A. Gitao, C.G. Bisimwa, P.N. Okafor, C. Bebora, C.L. capripox capripoxvirus democratic republic of the congo goat phylogeny coinfection Objective: We aimed at determining the prevalence and characterizing the CaPV, determining the CaPV-PPRV coinfection prevalence and providing data about phylogenetic relationship between the fusion protein of PPRV and P32 gene of CaPV. Materials and methods: A total of 150 samples including animals swabs, tissues and blood were collected from unvaccinated goats in a PPR and/or Capripox outbreaks in South Kivu, Eastern of Democratic Republic of the Congo. Conventional PCR and reverse transcriptase (RT-PCR) were used respectively to amplify P32, RPO30, GPCR genes of Capripox virus and Fusion (F) protein of PPRV. Positive samples were sequenced for phylogenetic analysis. Results: Out of 150 tested animals, 64.7% (n=97/150) were PPRV positive, 52.7% (n=79/150) were Capripox positive and 38.7% (n=58/150) were positive for both PPRV and CaPV. The pairwise comparison of P32gene of CaPV and F gene of PPRV showed 99.75% of identity percentage among goatpox virus sequences, 96.95% among PPRV sequences and 47.91% between CaPV and PPRV sequences. Conclusion: The study has demonstrated high prevalence of CaP V-PPRV mixed infection in South Kivu. Lumpy skinvirus disease (LSVD) is a lineage circulating which has a genetic relationship between its P32gene and the Fgene of PPRV giving the challenge to differentiate the two diseases at the clinical farm level. 2017 2018-05-16T10:38:55Z 2018-05-16T10:38:55Z Journal Article https://hdl.handle.net/10568/92569 en Open Access application/pdf ScopeMed Birindwa, B.A., Gitao, C.G., Bisimwa, P.N., Okafor, C. & Bebora, C.L. (2017). Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo. Journal of Advanced Veterinary and Animal Research, 4(4), 348-355. |
| spellingShingle | capripox capripoxvirus democratic republic of the congo goat phylogeny coinfection Birindwa, B.A. Gitao, C.G. Bisimwa, P.N. Okafor, C. Bebora, C.L. Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo |
| title | Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo |
| title_full | Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo |
| title_fullStr | Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo |
| title_full_unstemmed | Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo |
| title_short | Mixed infection of peste-des-petits ruminants and Capripox in goats in south Kivu, Democratic Republic of Congo |
| title_sort | mixed infection of peste des petits ruminants and capripox in goats in south kivu democratic republic of congo |
| topic | capripox capripoxvirus democratic republic of the congo goat phylogeny coinfection |
| url | https://hdl.handle.net/10568/92569 |
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