Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions
Background: An in vitro digestion and Caco-2 cell model may predict iron bioavailability to humans; however, direct comparisons are lacking. Objective: The objective was to test the differences in iron bioavailability between 2 maize varieties and 2 bean varieties (white beans and colored beans)...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2007
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/92544 |
| _version_ | 1855532838490734592 |
|---|---|
| author | Beiseigel, J.M. Hunt, J.R. Glahn, Raymond P. Welch, R.M. Menkir, A. Maziya-Dixon, B.B. |
| author_browse | Beiseigel, J.M. Glahn, Raymond P. Hunt, J.R. Maziya-Dixon, B.B. Menkir, A. Welch, R.M. |
| author_facet | Beiseigel, J.M. Hunt, J.R. Glahn, Raymond P. Welch, R.M. Menkir, A. Maziya-Dixon, B.B. |
| author_sort | Beiseigel, J.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: An in vitro digestion and Caco-2 cell model may predict iron bioavailability to humans; however, direct comparisons are lacking.
Objective: The objective was to test the differences in iron bioavailability between 2 maize varieties and 2 bean varieties (white beans and colored beans) by comparing human, Caco-2, and algorithm results.
Design: Two randomized, 2 × 2 factorial experiments compared women's iron absorption from 2 maize varieties (ACR and TZB; n = 26) and 2 bean varieties (great northern and pinto; n = 13), each fed with and without ascorbic acid (AA) from orange juice. Nonheme iron bioavailability was determined from 2-wk retention of extrinsic radioiron tracers and was compared with Caco-2 cell and algorithm results from identical meals.
Results: Without AA supplementation, women absorbed only about 2% of the iron from the maize or bean meals. The results were unaffected by the variety of either maize or beans. Adding AA (15–20 molar ratios of AA:iron) roughly tripled the iron absorption (P < 0.0001) from all test meals. Although the Caco-2 model predicted a slightly improved bioavailability of iron from ACR maize than from TZB maize (P < 0.05), it accurately predicted relative iron absorption from the maize meals. However, the Caco-2 model inaccurately predicted both a considerable difference between bean varieties (P < 0.0001) and a strong interaction between bean varieties and enhancement by AA (P < 0.0001). The algorithm method was more qualitatively than quantitatively useful and requires further development to accurately predict the influence of polyphenols on iron absorption.
Conclusions: Caco-2 predictions confirmed human iron absorption results for maize meals but not for bean meals, and algorithm predictions were only qualitatively predictive. |
| format | Journal Article |
| id | CGSpace92544 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| record_format | dspace |
| spelling | CGSpace925442025-08-01T16:06:41Z Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions Beiseigel, J.M. Hunt, J.R. Glahn, Raymond P. Welch, R.M. Menkir, A. Maziya-Dixon, B.B. bioavailability ascorbic acid phytic acid polyphenols tannins beans maize Background: An in vitro digestion and Caco-2 cell model may predict iron bioavailability to humans; however, direct comparisons are lacking. Objective: The objective was to test the differences in iron bioavailability between 2 maize varieties and 2 bean varieties (white beans and colored beans) by comparing human, Caco-2, and algorithm results. Design: Two randomized, 2 × 2 factorial experiments compared women's iron absorption from 2 maize varieties (ACR and TZB; n = 26) and 2 bean varieties (great northern and pinto; n = 13), each fed with and without ascorbic acid (AA) from orange juice. Nonheme iron bioavailability was determined from 2-wk retention of extrinsic radioiron tracers and was compared with Caco-2 cell and algorithm results from identical meals. Results: Without AA supplementation, women absorbed only about 2% of the iron from the maize or bean meals. The results were unaffected by the variety of either maize or beans. Adding AA (15–20 molar ratios of AA:iron) roughly tripled the iron absorption (P < 0.0001) from all test meals. Although the Caco-2 model predicted a slightly improved bioavailability of iron from ACR maize than from TZB maize (P < 0.05), it accurately predicted relative iron absorption from the maize meals. However, the Caco-2 model inaccurately predicted both a considerable difference between bean varieties (P < 0.0001) and a strong interaction between bean varieties and enhancement by AA (P < 0.0001). The algorithm method was more qualitatively than quantitatively useful and requires further development to accurately predict the influence of polyphenols on iron absorption. Conclusions: Caco-2 predictions confirmed human iron absorption results for maize meals but not for bean meals, and algorithm predictions were only qualitatively predictive. 2007 2018-05-14T14:35:44Z 2018-05-14T14:35:44Z Journal Article https://hdl.handle.net/10568/92544 en Limited Access Beiseigel, J.M., Hunt, J.R., Glahn, R.P., Welch, R., Menkir, A.& Maziya-Dixon, B.B. (2007). Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions. The American Journal of Clinical Nutrition, 86(2), 388-396. |
| spellingShingle | bioavailability ascorbic acid phytic acid polyphenols tannins beans maize Beiseigel, J.M. Hunt, J.R. Glahn, Raymond P. Welch, R.M. Menkir, A. Maziya-Dixon, B.B. Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions |
| title | Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions |
| title_full | Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions |
| title_fullStr | Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions |
| title_full_unstemmed | Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions |
| title_short | Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions |
| title_sort | iron bioavailability from maize and beans a comparison of human measurements with caco 2 cell and algorithm predictions |
| topic | bioavailability ascorbic acid phytic acid polyphenols tannins beans maize |
| url | https://hdl.handle.net/10568/92544 |
| work_keys_str_mv | AT beiseigeljm ironbioavailabilityfrommaizeandbeansacomparisonofhumanmeasurementswithcaco2cellandalgorithmpredictions AT huntjr ironbioavailabilityfrommaizeandbeansacomparisonofhumanmeasurementswithcaco2cellandalgorithmpredictions AT glahnraymondp ironbioavailabilityfrommaizeandbeansacomparisonofhumanmeasurementswithcaco2cellandalgorithmpredictions AT welchrm ironbioavailabilityfrommaizeandbeansacomparisonofhumanmeasurementswithcaco2cellandalgorithmpredictions AT menkira ironbioavailabilityfrommaizeandbeansacomparisonofhumanmeasurementswithcaco2cellandalgorithmpredictions AT maziyadixonbb ironbioavailabilityfrommaizeandbeansacomparisonofhumanmeasurementswithcaco2cellandalgorithmpredictions |