Comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers
Several maize near-isogenic inbred lines were subjected to comparative proteomics to identify kernel proteins associated with resistance. These lines were developed in Nigeria through a joint project between ARS-SRRC and the International Institute of Tropical Agriculture. The goal of this project i...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2007
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/92185 |
| _version_ | 1855515288708055040 |
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| author | Brown, R.L. Menkir, A. Bandyopadhyay, Ranajit Cleveland, T.E. Chen, Z. |
| author_browse | Bandyopadhyay, Ranajit Brown, R.L. Chen, Z. Cleveland, T.E. Menkir, A. |
| author_facet | Brown, R.L. Menkir, A. Bandyopadhyay, Ranajit Cleveland, T.E. Chen, Z. |
| author_sort | Brown, R.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Several maize near-isogenic inbred lines were subjected to comparative proteomics to identify kernel proteins associated with resistance. These lines were developed in Nigeria through a joint project between ARS-SRRC and the International Institute of Tropical Agriculture. The goal of this project is to develop aflatoxin-resistant inbreds for use in Central and West African national programs and U.S. breeding programs to combat aflatoxincontamination of maize. Parental lines that produced these inbreds were U.S. aflatoxin-resistant lines crossed with Central and West African lines selected for moderate to high ear rot resistance. Lines were selfed and selected for foliar and ear rot resistances and for good agronomic characteristics until the S4 generation where selection began for aflatoxin accumulation using a laboratory-based kernel screening assay (KSA) and field trials. Recently, inbred lines developed through the above-protocol were re-examined with the KSA and near-isogenic lines varying significantly in aflatoxin accumulation were identified. These lines were subjected to comparative proteomics and differences in protein expression between these near-isogenics were identified and results are discussed. |
| format | Journal Article |
| id | CGSpace92185 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| record_format | dspace |
| spelling | CGSpace921852023-06-12T09:09:05Z Comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers Brown, R.L. Menkir, A. Bandyopadhyay, Ranajit Cleveland, T.E. Chen, Z. maize inbred lines aflatoxin-resistant ear rot resistance kernel screening assay near-isogenic proteomics Several maize near-isogenic inbred lines were subjected to comparative proteomics to identify kernel proteins associated with resistance. These lines were developed in Nigeria through a joint project between ARS-SRRC and the International Institute of Tropical Agriculture. The goal of this project is to develop aflatoxin-resistant inbreds for use in Central and West African national programs and U.S. breeding programs to combat aflatoxincontamination of maize. Parental lines that produced these inbreds were U.S. aflatoxin-resistant lines crossed with Central and West African lines selected for moderate to high ear rot resistance. Lines were selfed and selected for foliar and ear rot resistances and for good agronomic characteristics until the S4 generation where selection began for aflatoxin accumulation using a laboratory-based kernel screening assay (KSA) and field trials. Recently, inbred lines developed through the above-protocol were re-examined with the KSA and near-isogenic lines varying significantly in aflatoxin accumulation were identified. These lines were subjected to comparative proteomics and differences in protein expression between these near-isogenics were identified and results are discussed. 2007 2018-04-24T08:40:11Z 2018-04-24T08:40:11Z Journal Article https://hdl.handle.net/10568/92185 en Limited Access Brown, R.L., Menkir, A., Bandyopadhyay, R., Cleveland, T.E. & Chen, Z. (2007). Comparative proteomics of near-isogenic maize inbred lines to identify potential aflatoxin-resistance markers. Phytopathology, 97(7), S14-S14. |
| spellingShingle | maize inbred lines aflatoxin-resistant ear rot resistance kernel screening assay near-isogenic proteomics Brown, R.L. Menkir, A. Bandyopadhyay, Ranajit Cleveland, T.E. Chen, Z. Comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers |
| title | Comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers |
| title_full | Comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers |
| title_fullStr | Comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers |
| title_full_unstemmed | Comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers |
| title_short | Comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers |
| title_sort | comparative proteomics of nearisogenic maize inbred lines to identify potential aflatoxinresistance markers |
| topic | maize inbred lines aflatoxin-resistant ear rot resistance kernel screening assay near-isogenic proteomics |
| url | https://hdl.handle.net/10568/92185 |
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