Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina
Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV) was identified as a mechanically transmitted potexvirus (Fam. Alphaflexiviridae). In Argentina, cassava is grown mainly in the North...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Wiley
2018
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| Online Access: | https://hdl.handle.net/10568/92138 |
| _version_ | 1855519215042166784 |
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| author | Zanini, A.A Cuéllar, Wilmer Jose Celli, M.G. Luque, V.A. Medina, R. Conci, V.C. Di Feo, L.V. del |
| author_browse | Celli, M.G. Conci, V.C. Cuéllar, Wilmer Jose Di Feo, L.V. del Luque, V.A. Medina, R. Zanini, A.A |
| author_facet | Zanini, A.A Cuéllar, Wilmer Jose Celli, M.G. Luque, V.A. Medina, R. Conci, V.C. Di Feo, L.V. del |
| author_sort | Zanini, A.A |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV) was identified as a mechanically transmitted potexvirus (Fam. Alphaflexiviridae). In Argentina, cassava is grown mainly in the North East region (NEA) that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated to severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work we successfully purified the virus and developed a double-antibody sandwich (DAS) ELISA test from an Argentinean field-isolate of CsCMV, to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate cluster was formed by sequences isolates from NEA samples which only share 77.1 to 80.3% nucleotide identity with the other clusters. These results indicated the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America. |
| format | Journal Article |
| id | CGSpace92138 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace921382025-11-12T05:54:04Z Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina Zanini, A.A Cuéllar, Wilmer Jose Celli, M.G. Luque, V.A. Medina, R. Conci, V.C. Di Feo, L.V. del manihot esculenta african cassava mosaic virus virus del mosaico de la cassava viruses plant diseases enfermedades de las plantas plant viruses virus de las plantas horticulture genetics Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV) was identified as a mechanically transmitted potexvirus (Fam. Alphaflexiviridae). In Argentina, cassava is grown mainly in the North East region (NEA) that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated to severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work we successfully purified the virus and developed a double-antibody sandwich (DAS) ELISA test from an Argentinean field-isolate of CsCMV, to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate cluster was formed by sequences isolates from NEA samples which only share 77.1 to 80.3% nucleotide identity with the other clusters. These results indicated the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America. 2018-10 2018-04-23T19:32:42Z 2018-04-23T19:32:42Z Journal Article https://hdl.handle.net/10568/92138 en Open Access application/pdf Wiley Zanini, A.A, Cuellar, Wilmer J. ,Celli, M.G., Luque, V.A., Medina, R., Conci, V.C., del Di Feo, L.V. 2018. Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina. Plant Pathology, 67(8): 1814-1820. |
| spellingShingle | manihot esculenta african cassava mosaic virus virus del mosaico de la cassava viruses plant diseases enfermedades de las plantas plant viruses virus de las plantas horticulture genetics Zanini, A.A Cuéllar, Wilmer Jose Celli, M.G. Luque, V.A. Medina, R. Conci, V.C. Di Feo, L.V. del Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina |
| title | Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina |
| title_full | Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina |
| title_fullStr | Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina |
| title_full_unstemmed | Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina |
| title_short | Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina |
| title_sort | distinct strains of the re emergent cassava common mosaic virus genus potexvirus infecting cassava in argentina |
| topic | manihot esculenta african cassava mosaic virus virus del mosaico de la cassava viruses plant diseases enfermedades de las plantas plant viruses virus de las plantas horticulture genetics |
| url | https://hdl.handle.net/10568/92138 |
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