Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers

Breeding efforts to develop high iron and zinc potatoes require a fast and inexpensive technique to evaluate mineral concentrations in large numbers of samples. In this study, we evaluated the feasibility of applying X-ray fluorescence spectrometry to estimate iron and zinc in freeze-dried and mille...

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Main Authors: Sosa, P., Guild, G., Burgos, G., Felde, T. zum, Bonierbale, Merideth W.
Format: Journal Article
Language:Inglés
Published: Elsevier 2018
Subjects:
Online Access:https://hdl.handle.net/10568/92129
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author Sosa, P.
Guild, G.
Burgos, G.
Felde, T. zum
Bonierbale, Merideth W.
author_browse Bonierbale, Merideth W.
Burgos, G.
Felde, T. zum
Guild, G.
Sosa, P.
author_facet Sosa, P.
Guild, G.
Burgos, G.
Felde, T. zum
Bonierbale, Merideth W.
author_sort Sosa, P.
collection Repository of Agricultural Research Outputs (CGSpace)
description Breeding efforts to develop high iron and zinc potatoes require a fast and inexpensive technique to evaluate mineral concentrations in large numbers of samples. In this study, we evaluated the feasibility of applying X-ray fluorescence spectrometry to estimate iron and zinc in freeze-dried and milled potato tuber samples. The calibration, and the external and independent validations showed high coefficients of determination, 0.93–0.96 for iron and 0.92–0.97 for zinc, and low standard errors, 1.10–1.44 mg/kg DW for iron and 0.91–1.06 mg/kg DW for zinc concentrations, indicating that iron and zinc can be estimated by X-ray fluorescence spectrometry with high precision. The developed calibrations were applied to estimate iron and zinc concentrations of hundreds of biofortified potato clones from the International Potato Center’s breeding program, grown in 3 distinct locations of Peru. Twenty clones showing high iron concentration (above 32 mg/kg DW) and 13 clones with high concentration of zinc (above 25 mg/kg DW) were identified. X-ray fluorescence spectrometry provides a rapid, low cost and suitable tool for potato breeders, compared to inductively coupled plasma optical emission spectrometry for screening iron and zinc concentrations, especially when a high number of potato clones must be evaluated in a short time frame.
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spelling CGSpace921292024-05-01T08:17:10Z Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers Sosa, P. Guild, G. Burgos, G. Felde, T. zum Bonierbale, Merideth W. potatoes iron zinc breeding food composition spectrometry Breeding efforts to develop high iron and zinc potatoes require a fast and inexpensive technique to evaluate mineral concentrations in large numbers of samples. In this study, we evaluated the feasibility of applying X-ray fluorescence spectrometry to estimate iron and zinc in freeze-dried and milled potato tuber samples. The calibration, and the external and independent validations showed high coefficients of determination, 0.93–0.96 for iron and 0.92–0.97 for zinc, and low standard errors, 1.10–1.44 mg/kg DW for iron and 0.91–1.06 mg/kg DW for zinc concentrations, indicating that iron and zinc can be estimated by X-ray fluorescence spectrometry with high precision. The developed calibrations were applied to estimate iron and zinc concentrations of hundreds of biofortified potato clones from the International Potato Center’s breeding program, grown in 3 distinct locations of Peru. Twenty clones showing high iron concentration (above 32 mg/kg DW) and 13 clones with high concentration of zinc (above 25 mg/kg DW) were identified. X-ray fluorescence spectrometry provides a rapid, low cost and suitable tool for potato breeders, compared to inductively coupled plasma optical emission spectrometry for screening iron and zinc concentrations, especially when a high number of potato clones must be evaluated in a short time frame. 2018-07-01 2018-04-23T01:43:31Z 2018-04-23T01:43:31Z Journal Article https://hdl.handle.net/10568/92129 en Open Access Elsevier Sosa, P.; Guild, G.; Burgos, G.; Bonierbale, M.; Felde, T.zum. 2018. Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers. Journal of Food Composition and Analysis. (USA). ISSN 0889-1575. 70:22-27.
spellingShingle potatoes
iron
zinc
breeding
food composition
spectrometry
Sosa, P.
Guild, G.
Burgos, G.
Felde, T. zum
Bonierbale, Merideth W.
Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers
title Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers
title_full Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers
title_fullStr Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers
title_full_unstemmed Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers
title_short Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers
title_sort potential and application of x ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers
topic potatoes
iron
zinc
breeding
food composition
spectrometry
url https://hdl.handle.net/10568/92129
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