Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding
Common bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption and is a major nutrition source in the tropics. Because bean production is reduced by both abiotic and biotic constraints, current breeding efforts are focused on the development of improved varieties with t...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2018
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/92030 |
| _version_ | 1855528481320861696 |
|---|---|
| author | Lobaton, Juan David Miller, Tamara Gil, Juanita Ariza, Daniel Hoz, Juan Fernando de la Soler Garzón, Alvaro Beebe, Stephen E. Duitama, Jorge Gepts, Paul L. Raatz, Bodo |
| author_browse | Ariza, Daniel Beebe, Stephen E. Duitama, Jorge Gepts, Paul L. Gil, Juanita Hoz, Juan Fernando de la Lobaton, Juan David Miller, Tamara Raatz, Bodo Soler Garzón, Alvaro |
| author_facet | Lobaton, Juan David Miller, Tamara Gil, Juanita Ariza, Daniel Hoz, Juan Fernando de la Soler Garzón, Alvaro Beebe, Stephen E. Duitama, Jorge Gepts, Paul L. Raatz, Bodo |
| author_sort | Lobaton, Juan David |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Common bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption and is a major nutrition source in the tropics. Because bean production is reduced by both abiotic and biotic constraints, current breeding efforts are focused on the development of improved varieties with tolerance to these stresses. We characterized materials from different breeding programs spanning three continents to understand their sequence diversity and advance the development of molecular breeding tools. For this, 37 varieties belonging to P. vulgaris, Phaseolus acutifolius (A. Gray), and Phaseolus coccineus L. were sequenced by whole-genome sequencing, identifying more than 40 million genomic variants. Evaluation of nuclear DNA content and analysis of copy number variation revealed important differences in genomic content not only between P. vulgaris and the two other domesticated Phaseolus species, but also within P. vulgaris, affecting hundreds of protein-coding genomic regions. A large number of inter–gene pool introgressions were identified. Furthermore, interspecific introgressions for disease resistance in breeding lines were mapped. Evaluation of newly developed single nucleotide polymorphism markers within previously discovered quantitative trait loci for common bacterial blight and angular leaf spot provides improved specificity to tag sources of resistance to these diseases. We expect that this dataset will provide a deeper molecular understanding of breeding germplasm and deliver molecular tools for germplasm development, aiming to increase the efficiency of bean breeding programs. |
| format | Journal Article |
| id | CGSpace92030 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace920302025-03-13T09:44:27Z Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding Lobaton, Juan David Miller, Tamara Gil, Juanita Ariza, Daniel Hoz, Juan Fernando de la Soler Garzón, Alvaro Beebe, Stephen E. Duitama, Jorge Gepts, Paul L. Raatz, Bodo common bean phaseolus vulgaris l. plant breeding fitomejoramiento disease resistance resistencia a la enfermedad introgression introgresión polymorphism Common bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption and is a major nutrition source in the tropics. Because bean production is reduced by both abiotic and biotic constraints, current breeding efforts are focused on the development of improved varieties with tolerance to these stresses. We characterized materials from different breeding programs spanning three continents to understand their sequence diversity and advance the development of molecular breeding tools. For this, 37 varieties belonging to P. vulgaris, Phaseolus acutifolius (A. Gray), and Phaseolus coccineus L. were sequenced by whole-genome sequencing, identifying more than 40 million genomic variants. Evaluation of nuclear DNA content and analysis of copy number variation revealed important differences in genomic content not only between P. vulgaris and the two other domesticated Phaseolus species, but also within P. vulgaris, affecting hundreds of protein-coding genomic regions. A large number of inter–gene pool introgressions were identified. Furthermore, interspecific introgressions for disease resistance in breeding lines were mapped. Evaluation of newly developed single nucleotide polymorphism markers within previously discovered quantitative trait loci for common bacterial blight and angular leaf spot provides improved specificity to tag sources of resistance to these diseases. We expect that this dataset will provide a deeper molecular understanding of breeding germplasm and deliver molecular tools for germplasm development, aiming to increase the efficiency of bean breeding programs. 2018-07 2018-04-06T16:32:55Z 2018-04-06T16:32:55Z Journal Article https://hdl.handle.net/10568/92030 en Open Access Wiley Lobaton, J. D., Miller, T., Gil, J., Ariza, D., de la Hoz, J. F., Soler, A., Beebe, S., Duitama, J., Gepts, P. and Raatz, B. (2018) Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding, Plant Genome, 11(2): 170068 |
| spellingShingle | common bean phaseolus vulgaris l. plant breeding fitomejoramiento disease resistance resistencia a la enfermedad introgression introgresión polymorphism Lobaton, Juan David Miller, Tamara Gil, Juanita Ariza, Daniel Hoz, Juan Fernando de la Soler Garzón, Alvaro Beebe, Stephen E. Duitama, Jorge Gepts, Paul L. Raatz, Bodo Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding |
| title | Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding |
| title_full | Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding |
| title_fullStr | Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding |
| title_full_unstemmed | Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding |
| title_short | Resequencing of common bean identifies regions of inter- gene pool introgression and provides comprehensive resources for molecular breeding |
| title_sort | resequencing of common bean identifies regions of inter gene pool introgression and provides comprehensive resources for molecular breeding |
| topic | common bean phaseolus vulgaris l. plant breeding fitomejoramiento disease resistance resistencia a la enfermedad introgression introgresión polymorphism |
| url | https://hdl.handle.net/10568/92030 |
| work_keys_str_mv | AT lobatonjuandavid resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT millertamara resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT giljuanita resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT arizadaniel resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT hozjuanfernandodela resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT solergarzonalvaro resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT beebestephene resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT duitamajorge resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT geptspaull resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding AT raatzbodo resequencingofcommonbeanidentifiesregionsofintergenepoolintrogressionandprovidescomprehensiveresourcesformolecularbreeding |