Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population
Quantitative trait loci (QTL) that confer broad‐spectrum resistance (BSR), or resistance that is effective against multiple and diverse plant pathogens, have been elusive targets of crop breeding programmes. Multiparent advanced generation intercross (MAGIC) populations, with their diverse genetic c...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/92027 |
| _version_ | 1855515349588377600 |
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| author | Bossa Castro, Ana M. Tekete, Cheick Raghavan, Chitra Delorean, Emily E. Dereeper, Alexis Dagno, Karim Koita, Ousmane Mosquera Cifuentes, Gloria Maria Leung, Hei Verdier, Valerie M. Leach, Jan E. |
| author_browse | Bossa Castro, Ana M. Dagno, Karim Delorean, Emily E. Dereeper, Alexis Koita, Ousmane Leach, Jan E. Leung, Hei Mosquera Cifuentes, Gloria Maria Raghavan, Chitra Tekete, Cheick Verdier, Valerie M. |
| author_facet | Bossa Castro, Ana M. Tekete, Cheick Raghavan, Chitra Delorean, Emily E. Dereeper, Alexis Dagno, Karim Koita, Ousmane Mosquera Cifuentes, Gloria Maria Leung, Hei Verdier, Valerie M. Leach, Jan E. |
| author_sort | Bossa Castro, Ana M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Quantitative trait loci (QTL) that confer broad‐spectrum resistance (BSR), or resistance that is effective against multiple and diverse plant pathogens, have been elusive targets of crop breeding programmes. Multiparent advanced generation intercross (MAGIC) populations, with their diverse genetic composition and high levels of recombination, are potential resources for the identification of QTL for BSR. In this study, a rice MAGIC population was used to map QTL conferring BSR to two major rice diseases, bacterial leaf streak (BLS) and bacterial blight (BB), caused by Xanthomonas oryzae pathovars (pv.) oryzicola (Xoc) and oryzae (Xoo), respectively. Controlling these diseases is particularly important in sub‐Saharan Africa, where no sources of BSR are currently available in deployed varieties. The MAGIC founders and lines were genotyped by sequencing and phenotyped in the greenhouse and field by inoculation with multiple strains of Xoc and Xoo. A combination of genomewide association studies (GWAS) and interval mapping analyses revealed 11 BSR QTL, effective against both diseases, and three pathovar‐specific QTL. The most promising BSR QTL (qXO‐2‐1, qXO‐4‐1 and qXO‐11‐2) conferred resistance to more than nine Xoc and Xoo strains. GWAS detected 369 significant SNP markers with distinguishable phenotypic effects, allowing the identification of alleles conferring disease resistance and susceptibility. The BSR and susceptibility QTL will improve our understanding of the mechanisms of both resistance and susceptibility in the long term and will be immediately useful resources for rice breeding programmes |
| format | Journal Article |
| id | CGSpace92027 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace920272025-03-13T09:43:59Z Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population Bossa Castro, Ana M. Tekete, Cheick Raghavan, Chitra Delorean, Emily E. Dereeper, Alexis Dagno, Karim Koita, Ousmane Mosquera Cifuentes, Gloria Maria Leung, Hei Verdier, Valerie M. Leach, Jan E. quantitative trait loci loci de rasgos cuantitativos xamthomonas oryzae bacterial leaf blight rice oryza sativa disease resistance resistencia a la enfermedad biotechnology Quantitative trait loci (QTL) that confer broad‐spectrum resistance (BSR), or resistance that is effective against multiple and diverse plant pathogens, have been elusive targets of crop breeding programmes. Multiparent advanced generation intercross (MAGIC) populations, with their diverse genetic composition and high levels of recombination, are potential resources for the identification of QTL for BSR. In this study, a rice MAGIC population was used to map QTL conferring BSR to two major rice diseases, bacterial leaf streak (BLS) and bacterial blight (BB), caused by Xanthomonas oryzae pathovars (pv.) oryzicola (Xoc) and oryzae (Xoo), respectively. Controlling these diseases is particularly important in sub‐Saharan Africa, where no sources of BSR are currently available in deployed varieties. The MAGIC founders and lines were genotyped by sequencing and phenotyped in the greenhouse and field by inoculation with multiple strains of Xoc and Xoo. A combination of genomewide association studies (GWAS) and interval mapping analyses revealed 11 BSR QTL, effective against both diseases, and three pathovar‐specific QTL. The most promising BSR QTL (qXO‐2‐1, qXO‐4‐1 and qXO‐11‐2) conferred resistance to more than nine Xoc and Xoo strains. GWAS detected 369 significant SNP markers with distinguishable phenotypic effects, allowing the identification of alleles conferring disease resistance and susceptibility. The BSR and susceptibility QTL will improve our understanding of the mechanisms of both resistance and susceptibility in the long term and will be immediately useful resources for rice breeding programmes 2018-09 2018-04-04T20:55:23Z 2018-04-04T20:55:23Z Journal Article https://hdl.handle.net/10568/92027 en Open Access Wiley Bossa-Castro, A. M., Tekete, C., Raghavan, C., Delorean, E. E., Dereeper, A., Dagno, K., Koita, O., Mosquera, G., Leung, H., Verdier, V. and Leach, J. E. (2018) Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population, Plant Biotechnology Journal, 16(9): 1559-1568. |
| spellingShingle | quantitative trait loci loci de rasgos cuantitativos xamthomonas oryzae bacterial leaf blight rice oryza sativa disease resistance resistencia a la enfermedad biotechnology Bossa Castro, Ana M. Tekete, Cheick Raghavan, Chitra Delorean, Emily E. Dereeper, Alexis Dagno, Karim Koita, Ousmane Mosquera Cifuentes, Gloria Maria Leung, Hei Verdier, Valerie M. Leach, Jan E. Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population |
| title | Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population |
| title_full | Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population |
| title_fullStr | Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population |
| title_full_unstemmed | Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population |
| title_short | Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population |
| title_sort | allelic variation for broad spectrum resistance and susceptibility to bacterial pathogens identified in a rice magic population |
| topic | quantitative trait loci loci de rasgos cuantitativos xamthomonas oryzae bacterial leaf blight rice oryza sativa disease resistance resistencia a la enfermedad biotechnology |
| url | https://hdl.handle.net/10568/92027 |
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