Evolution of quality standards in plant cryobanks - The International Potato Center as a case study
The International Potato Center holds one of the largest and most diverse potato cryobanks worldwide. The cryo-collection includes 1855 landraces, belonging to seven potato species and four different ploidy levels. The potato cryo-collection was principally built up in recent years (2013-17), under...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/92010 |
| _version_ | 1855540397307068416 |
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| author | Vollmer, R. Ellis, David Barkley, N.A. |
| author_browse | Barkley, N.A. Ellis, David Vollmer, R. |
| author_facet | Vollmer, R. Ellis, David Barkley, N.A. |
| author_sort | Vollmer, R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The International Potato Center holds one of the largest and most diverse potato cryobanks worldwide. The cryo-collection includes 1855 landraces, belonging to seven potato species and four different ploidy levels. The potato cryo-collection was principally built up in recent years (2013-17), under strict quality and operative standards. The average regrowth rate of the potato cryo-collection is 58% and 1234 of 1855 accessions showed a regrowth rate of 50% or higher. The success rate, i.e. the percent of successfully cryopreserved accessions (minimum 20% of regrowth) in relation to the totally processed accessions has increased in 2016 to 95.4%. Real time data record of each step of the process with pocket PCs and bi-dimensional cryo-resistant barcode labels is an important part of our cryo-system. Full plant recovery (+LN) as standard parameter for regrowth assessment, a large-scale applicable cryo-protocol (high success rate), systematic viability reassessment, clear threshold of minimum acceptable regrowth (30%: one single cryo-run, 20-30%: two independent cryo-runs, <20%: accession was discarded and not transferred to the cryobank), a high sample size (20%) for initial regrowth assessment, safety back-up of cryobanked accessions, performance indicators of the cryo-staff, and a continuous electronical monitoring of the correct temperature within the cryotanks are other important features of the system. It was concluded that the International Potato Center has built up a quality system for cryobanking that has integrated the key components for good cryobanking of Plant Genetic Resources. |
| format | Journal Article |
| id | CGSpace92010 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace920102024-11-14T08:05:31Z Evolution of quality standards in plant cryobanks - The International Potato Center as a case study Vollmer, R. Ellis, David Barkley, N.A. potatoes cryopreservation organization of research gene banks The International Potato Center holds one of the largest and most diverse potato cryobanks worldwide. The cryo-collection includes 1855 landraces, belonging to seven potato species and four different ploidy levels. The potato cryo-collection was principally built up in recent years (2013-17), under strict quality and operative standards. The average regrowth rate of the potato cryo-collection is 58% and 1234 of 1855 accessions showed a regrowth rate of 50% or higher. The success rate, i.e. the percent of successfully cryopreserved accessions (minimum 20% of regrowth) in relation to the totally processed accessions has increased in 2016 to 95.4%. Real time data record of each step of the process with pocket PCs and bi-dimensional cryo-resistant barcode labels is an important part of our cryo-system. Full plant recovery (+LN) as standard parameter for regrowth assessment, a large-scale applicable cryo-protocol (high success rate), systematic viability reassessment, clear threshold of minimum acceptable regrowth (30%: one single cryo-run, 20-30%: two independent cryo-runs, <20%: accession was discarded and not transferred to the cryobank), a high sample size (20%) for initial regrowth assessment, safety back-up of cryobanked accessions, performance indicators of the cryo-staff, and a continuous electronical monitoring of the correct temperature within the cryotanks are other important features of the system. It was concluded that the International Potato Center has built up a quality system for cryobanking that has integrated the key components for good cryobanking of Plant Genetic Resources. 2018-02 2018-04-03T19:10:51Z 2018-04-03T19:10:51Z Journal Article https://hdl.handle.net/10568/92010 en Open Access Elsevier Vollmer, R.; Ellis, D.; Barkley, N. 2018. Evolution of quality standards in plant cryobanks - The International Potato Center as a case study. Cryobiology. (USA). ISSN 0011-2240. 80:163. |
| spellingShingle | potatoes cryopreservation organization of research gene banks Vollmer, R. Ellis, David Barkley, N.A. Evolution of quality standards in plant cryobanks - The International Potato Center as a case study |
| title | Evolution of quality standards in plant cryobanks - The International Potato Center as a case study |
| title_full | Evolution of quality standards in plant cryobanks - The International Potato Center as a case study |
| title_fullStr | Evolution of quality standards in plant cryobanks - The International Potato Center as a case study |
| title_full_unstemmed | Evolution of quality standards in plant cryobanks - The International Potato Center as a case study |
| title_short | Evolution of quality standards in plant cryobanks - The International Potato Center as a case study |
| title_sort | evolution of quality standards in plant cryobanks the international potato center as a case study |
| topic | potatoes cryopreservation organization of research gene banks |
| url | https://hdl.handle.net/10568/92010 |
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