Severity of citrus tristeza virus isolates from Texas

Citrus tristeza virus (CTV) isolates collected from the Lower Rio Grande Valley in south Texas and east Texas were characterized using citrus indicators and molecular methods. The citrus indicators were Mexican lime (Citrus aurantifolia), sour orange (C. aurantium), sweet orange (C. sinensis) grafte...

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Main Authors: Herron, C., Mirkov, T., Solis-Gracia, N., Kahlke, C., Skaria, M., Graca, J. da
Format: Journal Article
Language:Inglés
Published: Scientific Societies 2005
Subjects:
Online Access:https://hdl.handle.net/10568/91892
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author Herron, C.
Mirkov, T.
Solis-Gracia, N.
Kahlke, C.
Skaria, M.
Graca, J. da
author_browse Graca, J. da
Herron, C.
Kahlke, C.
Mirkov, T.
Skaria, M.
Solis-Gracia, N.
author_facet Herron, C.
Mirkov, T.
Solis-Gracia, N.
Kahlke, C.
Skaria, M.
Graca, J. da
author_sort Herron, C.
collection Repository of Agricultural Research Outputs (CGSpace)
description Citrus tristeza virus (CTV) isolates collected from the Lower Rio Grande Valley in south Texas and east Texas were characterized using citrus indicators and molecular methods. The citrus indicators were Mexican lime (Citrus aurantifolia), sour orange (C. aurantium), sweet orange (C. sinensis) grafted to sour orange, Duncan grapefruit (C. × paradisi), and Madam Vinous sweet orange, with some CTV isolates additionally indexed using the Texas commercial grapefruit cvs. Rio Red and Star Ruby, and Marrs and N-33 sweet orange. Severity ratings used 11 biotype groups or cumulative mean relative indices. Molecular characterization was carried out using poly- and monoclonal antibodies, seven strain-specific probes and single-stranded conformational polymorphism, and all were based on the CTV major coat protein or gene. All Texas CTV isolates produced vein clearing symptoms on inoculated Mexican lime plants. Over half of the CTV isolates tested were placed in biotype groups IX and X (causing decline of sweet orange on sour orange, seedling yellows on sour orange and grapefruit seedlings, and stem pitting of grapefruit or sweet orange), and one isolate was in biotype I (mild).
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spelling CGSpace918922024-05-15T05:11:41Z Severity of citrus tristeza virus isolates from Texas Herron, C. Mirkov, T. Solis-Gracia, N. Kahlke, C. Skaria, M. Graca, J. da citrus tristeza virus citrus indicators citrus aurantifolia molecular biotype group seedlings Citrus tristeza virus (CTV) isolates collected from the Lower Rio Grande Valley in south Texas and east Texas were characterized using citrus indicators and molecular methods. The citrus indicators were Mexican lime (Citrus aurantifolia), sour orange (C. aurantium), sweet orange (C. sinensis) grafted to sour orange, Duncan grapefruit (C. × paradisi), and Madam Vinous sweet orange, with some CTV isolates additionally indexed using the Texas commercial grapefruit cvs. Rio Red and Star Ruby, and Marrs and N-33 sweet orange. Severity ratings used 11 biotype groups or cumulative mean relative indices. Molecular characterization was carried out using poly- and monoclonal antibodies, seven strain-specific probes and single-stranded conformational polymorphism, and all were based on the CTV major coat protein or gene. All Texas CTV isolates produced vein clearing symptoms on inoculated Mexican lime plants. Over half of the CTV isolates tested were placed in biotype groups IX and X (causing decline of sweet orange on sour orange, seedling yellows on sour orange and grapefruit seedlings, and stem pitting of grapefruit or sweet orange), and one isolate was in biotype I (mild). 2005-06 2018-03-23T06:48:58Z 2018-03-23T06:48:58Z Journal Article https://hdl.handle.net/10568/91892 en Open Access Scientific Societies Herron, C., Mirkov, T., Solis-Gracia, N., Kahlke, C., Skaria, M. & Da Graca, J. (2005). Severity of citrus tristeza virus isolates from Texas. Plant Disease, 89(6), 575-580.
spellingShingle citrus tristeza virus
citrus indicators
citrus aurantifolia
molecular
biotype group
seedlings
Herron, C.
Mirkov, T.
Solis-Gracia, N.
Kahlke, C.
Skaria, M.
Graca, J. da
Severity of citrus tristeza virus isolates from Texas
title Severity of citrus tristeza virus isolates from Texas
title_full Severity of citrus tristeza virus isolates from Texas
title_fullStr Severity of citrus tristeza virus isolates from Texas
title_full_unstemmed Severity of citrus tristeza virus isolates from Texas
title_short Severity of citrus tristeza virus isolates from Texas
title_sort severity of citrus tristeza virus isolates from texas
topic citrus tristeza virus
citrus indicators
citrus aurantifolia
molecular
biotype group
seedlings
url https://hdl.handle.net/10568/91892
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