Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer

High necrosis and poor survival rate of target plant tissues are some of the major factors that affect the efficiency of Agrobacterium-mediated T-DNA transfer into plant cells. These factors may be the result of, or linked to, hypersensitive defense reaction in plants to Agrobacterium infection, whi...

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Autores principales: Kuta, D., Tripathi, L.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2005
Materias:
Acceso en línea:https://hdl.handle.net/10568/91813
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author Kuta, D.
Tripathi, L.
author_browse Kuta, D.
Tripathi, L.
author_facet Kuta, D.
Tripathi, L.
author_sort Kuta, D.
collection Repository of Agricultural Research Outputs (CGSpace)
description High necrosis and poor survival rate of target plant tissues are some of the major factors that affect the efficiency of Agrobacterium-mediated T-DNA transfer into plant cells. These factors may be the result of, or linked to, hypersensitive defense reaction in plants to Agrobacterium infection, which may involve the recognition of specific signals from the Agrobacterium that triggers the burst of reactive oxygen species at the infection site. Evidences of Agrobacterium-induced necrosis in target plant tissues and its link to reactive oxygen species are presented. Application of antioxidants, addition of acetosyringone and optimization of pre-culture conditions suppress the Agrobacterium-induced hypersensitive necrotic response in target plant tissues, thereby enhancing stable transformation.
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spelling CGSpace918132023-06-13T05:12:48Z Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer Kuta, D. Tripathi, L. agrobacterium hypersensitive reaction necrosis signal transduction oxidative burst transformation High necrosis and poor survival rate of target plant tissues are some of the major factors that affect the efficiency of Agrobacterium-mediated T-DNA transfer into plant cells. These factors may be the result of, or linked to, hypersensitive defense reaction in plants to Agrobacterium infection, which may involve the recognition of specific signals from the Agrobacterium that triggers the burst of reactive oxygen species at the infection site. Evidences of Agrobacterium-induced necrosis in target plant tissues and its link to reactive oxygen species are presented. Application of antioxidants, addition of acetosyringone and optimization of pre-culture conditions suppress the Agrobacterium-induced hypersensitive necrotic response in target plant tissues, thereby enhancing stable transformation. 2005 2018-03-23T06:48:49Z 2018-03-23T06:48:49Z Journal Article https://hdl.handle.net/10568/91813 en Open Access Kuta, D. & Tripathi, L. (2005). Agrobacterium-induced hypersensitive necrotic reaction in plant cells: a resistance response against Agrobacterium-mediated DNA transfer. African Journal of Biotechnology, 4(8), 752-757.
spellingShingle agrobacterium
hypersensitive reaction
necrosis
signal transduction
oxidative burst
transformation
Kuta, D.
Tripathi, L.
Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer
title Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer
title_full Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer
title_fullStr Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer
title_full_unstemmed Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer
title_short Agrobacteriuminduced hypersensitive nectrotic reaction in plant cells: a resistance response against Agrobacteriummediated DNA transfer
title_sort agrobacteriuminduced hypersensitive nectrotic reaction in plant cells a resistance response against agrobacteriummediated dna transfer
topic agrobacterium
hypersensitive reaction
necrosis
signal transduction
oxidative burst
transformation
url https://hdl.handle.net/10568/91813
work_keys_str_mv AT kutad agrobacteriuminducedhypersensitivenectroticreactioninplantcellsaresistanceresponseagainstagrobacteriummediateddnatransfer
AT tripathil agrobacteriuminducedhypersensitivenectroticreactioninplantcellsaresistanceresponseagainstagrobacteriummediateddnatransfer