Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)
Musa spp (bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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2006
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| Online Access: | https://hdl.handle.net/10568/91780 |
| _version_ | 1855529699553312768 |
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| author | Pillay, M. Ogundiwin, E. Tenkouano, A. Doležel, Jaroslav |
| author_browse | Doležel, Jaroslav Ogundiwin, E. Pillay, M. Tenkouano, A. |
| author_facet | Pillay, M. Ogundiwin, E. Tenkouano, A. Doležel, Jaroslav |
| author_sort | Pillay, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Musa spp (bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. This study determined the ploidy levels and genome composition of the Musa germplasm collection, constituting over 300 accessions, at the International Institute of Tropical Agriculture in Nigeria and Uganda. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels, while genome composition was ascertained with RAPD markers that are specific for the A and B genomes of Musa. It was determined that at least 8% of the plants in the germplasm collection were miss-classified in terms of ploidy and/or genome composition. The cultivars 'Pisang awak', 'Foulah 4' and 'Nzizi', previously classified as triploids, were found to be tetraploids by flow cytometry and conventional root tip chromosome counts. Similarly, cultivars that were previously classified as diploids including 'Too', and 'Toowoolee' were found to be triploids in our analysis. Ploidy and genome classification in Musa was generally determined from morphological characteristics. While our study showed that such a system is not always reliable, it was interesting to find that none of the plantains in the germplasm collection were miss-classified with regards to both ploidy and genome composition. |
| format | Journal Article |
| id | CGSpace91780 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| record_format | dspace |
| spelling | CGSpace917802023-06-12T16:22:12Z Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA) Pillay, M. Ogundiwin, E. Tenkouano, A. Doležel, Jaroslav bananas plantains genomes ploid Musa spp (bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. This study determined the ploidy levels and genome composition of the Musa germplasm collection, constituting over 300 accessions, at the International Institute of Tropical Agriculture in Nigeria and Uganda. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels, while genome composition was ascertained with RAPD markers that are specific for the A and B genomes of Musa. It was determined that at least 8% of the plants in the germplasm collection were miss-classified in terms of ploidy and/or genome composition. The cultivars 'Pisang awak', 'Foulah 4' and 'Nzizi', previously classified as triploids, were found to be tetraploids by flow cytometry and conventional root tip chromosome counts. Similarly, cultivars that were previously classified as diploids including 'Too', and 'Toowoolee' were found to be triploids in our analysis. Ploidy and genome classification in Musa was generally determined from morphological characteristics. While our study showed that such a system is not always reliable, it was interesting to find that none of the plantains in the germplasm collection were miss-classified with regards to both ploidy and genome composition. 2006 2018-03-23T06:48:45Z 2018-03-23T06:48:45Z Journal Article https://hdl.handle.net/10568/91780 en Open Access Pillay, M., Ogundiwin, E., Tenkouano, A. & Dolezel, J. (2006). Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA). African Journal of Biotechnology, 5(13), 1224-1232. |
| spellingShingle | bananas plantains genomes ploid Pillay, M. Ogundiwin, E. Tenkouano, A. Doležel, Jaroslav Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA) |
| title | Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA) |
| title_full | Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA) |
| title_fullStr | Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA) |
| title_full_unstemmed | Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA) |
| title_short | Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA) |
| title_sort | ploidy and genome composition of musa germplasm at the international institute of tropical agriculture iita |
| topic | bananas plantains genomes ploid |
| url | https://hdl.handle.net/10568/91780 |
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