Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets
Under laboratory conditions, nonpathogenic, endophytic Fusarium oxysporum inflicts high mortality among banana weevils and nematodes. Following inoculation into banana (Musa spp.) tissue cultured plants, successful colonisation is necessary for efficient biological control of these pests. The patter...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2006
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/91766 |
| _version_ | 1855524443034484736 |
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| author | Paparu, P. Dubois, T. Gold, C. Niere, B. Adipala, E. Coyne, Danny L. |
| author_browse | Adipala, E. Coyne, Danny L. Dubois, T. Gold, C. Niere, B. Paparu, P. |
| author_facet | Paparu, P. Dubois, T. Gold, C. Niere, B. Adipala, E. Coyne, Danny L. |
| author_sort | Paparu, P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Under laboratory conditions, nonpathogenic, endophytic Fusarium oxysporum inflicts high mortality among banana weevils and nematodes. Following inoculation into banana (Musa spp.) tissue cultured plants, successful colonisation is necessary for efficient biological control of these pests. The pattern of root and rhizome colonisation by two nonpathogenic Ugandan F. oxysporum strains (V2w2 and III4w1) in cv. Nabusa (AAA-EA) was investigated using light microscopy. Percentage of colonisation in the rhizomes (93%) was higher than in the roots (56%), but hyphal density in the roots (0.30 mm−2) was higher than in the rhizomes (0.21 mm−2). The root bases were better colonised (76%) than root midsections (53%) or tips (39%). Both the strains colonised the roots and the rhizomes, with numerous hyphae infecting the hypodermis but fewer infecting the cortex. Colonisation of vascular tissues was not recorded. Despite the presence of hyphae in intercellular and intracellular spaces of the roots and the rhizomes, normal cell structure was observed. Our report provides the first in situ observation and quantification of endophyte colonisation in banana. The study demonstrated the ability of F. oxysporum strains V2w2 and III4w1 to penetrate intact host tissues and recolonise the host internally upon inoculation, an important step for their suitability as biological control agents. |
| format | Journal Article |
| id | CGSpace91766 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace917662024-08-27T10:35:52Z Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets Paparu, P. Dubois, T. Gold, C. Niere, B. Adipala, E. Coyne, Danny L. bananas endophyte light microscopy biological control banana weevils tissue cultured Under laboratory conditions, nonpathogenic, endophytic Fusarium oxysporum inflicts high mortality among banana weevils and nematodes. Following inoculation into banana (Musa spp.) tissue cultured plants, successful colonisation is necessary for efficient biological control of these pests. The pattern of root and rhizome colonisation by two nonpathogenic Ugandan F. oxysporum strains (V2w2 and III4w1) in cv. Nabusa (AAA-EA) was investigated using light microscopy. Percentage of colonisation in the rhizomes (93%) was higher than in the roots (56%), but hyphal density in the roots (0.30 mm−2) was higher than in the rhizomes (0.21 mm−2). The root bases were better colonised (76%) than root midsections (53%) or tips (39%). Both the strains colonised the roots and the rhizomes, with numerous hyphae infecting the hypodermis but fewer infecting the cortex. Colonisation of vascular tissues was not recorded. Despite the presence of hyphae in intercellular and intracellular spaces of the roots and the rhizomes, normal cell structure was observed. Our report provides the first in situ observation and quantification of endophyte colonisation in banana. The study demonstrated the ability of F. oxysporum strains V2w2 and III4w1 to penetrate intact host tissues and recolonise the host internally upon inoculation, an important step for their suitability as biological control agents. 2006-08 2018-03-23T06:48:43Z 2018-03-23T06:48:43Z Journal Article https://hdl.handle.net/10568/91766 en Limited Access Wiley Paparu, P., Dubois, T., Gold, C., Niere, B., Adipala, E. & Coyne, D. (2006). Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets. Annals of Applied Biology, 149(1), 1-8. |
| spellingShingle | bananas endophyte light microscopy biological control banana weevils tissue cultured Paparu, P. Dubois, T. Gold, C. Niere, B. Adipala, E. Coyne, Danny L. Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets |
| title | Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets |
| title_full | Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets |
| title_fullStr | Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets |
| title_full_unstemmed | Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets |
| title_short | Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets |
| title_sort | colonisation pattern of nonpathogenic fusarium oxysporum a potential biological control agent in roots and rhizomes of tissue cultured musa plantlets |
| topic | bananas endophyte light microscopy biological control banana weevils tissue cultured |
| url | https://hdl.handle.net/10568/91766 |
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