Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS)

An alternative to conventional cultivation of rice on submerged paddy soil is the ground cover rice production system (GCRPS), in which soil is covered with a plastic film to reduce the use of irrigation water. However, reduced soil water, increased aeration and temperature under GCRPS could promote...

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Autores principales: Zhe Chen, Shan Lin, Zhisheng Yao, Xunhua Zheng, Gschwendtner, S., Schloterd, M., Meiju Liu, Yanan Zhangb, Butterbach-Bahl, Klaus, Dannenmann, Michael
Formato: Journal Article
Lenguaje:Inglés
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://hdl.handle.net/10568/91674
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author Zhe Chen
Shan Lin
Zhisheng Yao
Xunhua Zheng
Gschwendtner, S.
Schloterd, M.
Meiju Liu
Yanan Zhangb
Butterbach-Bahl, Klaus
Dannenmann, Michael
author_browse Butterbach-Bahl, Klaus
Dannenmann, Michael
Gschwendtner, S.
Meiju Liu
Schloterd, M.
Shan Lin
Xunhua Zheng
Yanan Zhangb
Zhe Chen
Zhisheng Yao
author_facet Zhe Chen
Shan Lin
Zhisheng Yao
Xunhua Zheng
Gschwendtner, S.
Schloterd, M.
Meiju Liu
Yanan Zhangb
Butterbach-Bahl, Klaus
Dannenmann, Michael
author_sort Zhe Chen
collection Repository of Agricultural Research Outputs (CGSpace)
description An alternative to conventional cultivation of rice on submerged paddy soil is the ground cover rice production system (GCRPS), in which soil is covered with a plastic film to reduce the use of irrigation water. However, reduced soil water, increased aeration and temperature under GCRPS could promote soil nitrogen (N) mineralizing, nitrifying and denitrifying microbes and thus enhance soil N turnover and environmental losses e.g., through emission of the potent greenhouse gas nitrous oxide (N2O). At two sites with paired GCRPS and conventional paddy fields in Central China, we followed the abundance and activity of N-mineralizers, nitrifiers, denitrifiers and N2-fixing microbes based on qPCR from DNA and RNA directly extracted from soil. With decreasing soil water during the growing season, GCRPS strongly increased N mineralization as illustrated by several fold increased transcript levels of chiA. Furthermore, GCRPS reduced the nifH transcripts (encoding for nitrogenase) by 38% to 70% but increased the qnorB transcripts by 160% and archaeal amoA (AOA) transcripts by one order of magnitude (encoding for nitric oxide reductase and ammonia monooxygenase). This indicated a higher potential for N losses due to decreased biological N2 fixation, increased N leaching and increased N2O emission in GCRPS. The latter was confirmed by increased in situ N2O emissions. In addition, the N2-fixing and denitrifying microbial community composition as measured by a community fingerprinting approach was strongly influenced by GCRPS cultivation. Hence, our study reveals the microbial mechanisms underlying the risks for increased N mineralization, nitrification and N2O emissions and decreased biological N fixation in GCRPS. However, analysis of topsoil N stocks provided evidence that at least under N fertilizer application, GCRPS might overall maintain soil N stocks. This might result from a GCRPS-induced increase in fertilizer N use efficiency, root development and C and N return via residues, which appear to outbalance the observed effects on nitrification, gaseous N losses and biological N fixation, thereby preventing a net loss of total soil N.
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spelling CGSpace916742024-01-17T12:58:34Z Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS) Zhe Chen Shan Lin Zhisheng Yao Xunhua Zheng Gschwendtner, S. Schloterd, M. Meiju Liu Yanan Zhangb Butterbach-Bahl, Klaus Dannenmann, Michael crops rice irrigation water microbiology An alternative to conventional cultivation of rice on submerged paddy soil is the ground cover rice production system (GCRPS), in which soil is covered with a plastic film to reduce the use of irrigation water. However, reduced soil water, increased aeration and temperature under GCRPS could promote soil nitrogen (N) mineralizing, nitrifying and denitrifying microbes and thus enhance soil N turnover and environmental losses e.g., through emission of the potent greenhouse gas nitrous oxide (N2O). At two sites with paired GCRPS and conventional paddy fields in Central China, we followed the abundance and activity of N-mineralizers, nitrifiers, denitrifiers and N2-fixing microbes based on qPCR from DNA and RNA directly extracted from soil. With decreasing soil water during the growing season, GCRPS strongly increased N mineralization as illustrated by several fold increased transcript levels of chiA. Furthermore, GCRPS reduced the nifH transcripts (encoding for nitrogenase) by 38% to 70% but increased the qnorB transcripts by 160% and archaeal amoA (AOA) transcripts by one order of magnitude (encoding for nitric oxide reductase and ammonia monooxygenase). This indicated a higher potential for N losses due to decreased biological N2 fixation, increased N leaching and increased N2O emission in GCRPS. The latter was confirmed by increased in situ N2O emissions. In addition, the N2-fixing and denitrifying microbial community composition as measured by a community fingerprinting approach was strongly influenced by GCRPS cultivation. Hence, our study reveals the microbial mechanisms underlying the risks for increased N mineralization, nitrification and N2O emissions and decreased biological N fixation in GCRPS. However, analysis of topsoil N stocks provided evidence that at least under N fertilizer application, GCRPS might overall maintain soil N stocks. This might result from a GCRPS-induced increase in fertilizer N use efficiency, root development and C and N return via residues, which appear to outbalance the observed effects on nitrification, gaseous N losses and biological N fixation, thereby preventing a net loss of total soil N. 2018-06 2018-03-13T13:11:23Z 2018-03-13T13:11:23Z Journal Article https://hdl.handle.net/10568/91674 en Limited Access Elsevier Zhe Chen, Shan Lin, Zhisheng Yao, Xunhua Zheng, Gschwendtner, S., Schloterd, M., Meiju Liu, Yanan Zhangb, Butterbach-Bahla, K. and Dannenmann, M. 2018. Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS). Soil Biology and Biochemistry 121:77–86.
spellingShingle crops
rice
irrigation
water
microbiology
Zhe Chen
Shan Lin
Zhisheng Yao
Xunhua Zheng
Gschwendtner, S.
Schloterd, M.
Meiju Liu
Yanan Zhangb
Butterbach-Bahl, Klaus
Dannenmann, Michael
Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS)
title Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS)
title_full Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS)
title_fullStr Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS)
title_full_unstemmed Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS)
title_short Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS)
title_sort enhanced nitrogen cycling and n2o loss in water saving ground cover rice production systems gcrps
topic crops
rice
irrigation
water
microbiology
url https://hdl.handle.net/10568/91674
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