Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis
Radopholus similisis a major pest of East African highland cooking bananas (Musaspp.) in Uganda. Non-pathogenic Fusariumoxysporumendophytes, isolated from bananas in farmers’ fields, have shown potential to reduceR. Similis numbers in tissue culture banana. The mechanism through which endophytes con...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2007
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/91450 |
| _version_ | 1855540608547946496 |
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| author | Paparu, P. Dubois, T. Coyne, Danny L. Viljoen, Altus |
| author_browse | Coyne, Danny L. Dubois, T. Paparu, P. Viljoen, Altus |
| author_facet | Paparu, P. Dubois, T. Coyne, Danny L. Viljoen, Altus |
| author_sort | Paparu, P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Radopholus similisis a major pest of East African highland cooking bananas (Musaspp.) in Uganda. Non-pathogenic Fusariumoxysporumendophytes, isolated from bananas in farmers’ fields, have shown potential to reduceR. Similis numbers in tissue culture banana. The mechanism through which endophytes confer resistance to nematodes has previously been demonstrated to involve inducedresistance. In this study, the expression of eight defense-related genes in banana was investigated using quantitative real-time reverse transcription PCR. Plants of susceptible (cv. Nabusa, genomic group AAA-EA) and tolerant (cv. Kayinja, genomic group ABB) banana cultivars were inoculated with endophyticF. Oxysporum strain V5w2. Gene expression levels were analysed following endophyte inoculation and nematode challenge. Endophyte colonization of roots of the tolerant cultivar induced transient expression of POX and suppressed expression ofPR-3,lectin, PAE, PALandPIR7A. CatalaseandPR-1activities were up-regulated in the tolerant cultivar 33days after endophyte colonization of roots, but their expressions were further up-regulated following nematode challenge. Apart from POX andlectin, the other genes analysed were not responsive to endophyte colonization orR. Similis challenge in the susceptible cv.Nabusa. This is the first report of endophyte-induced defense-related gene expression in banana. |
| format | Journal Article |
| id | CGSpace91450 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace914502024-05-15T05:12:14Z Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis Paparu, P. Dubois, T. Coyne, Danny L. Viljoen, Altus bananas radopholus similis fungal endophyte fusarium oxysporum gene expression musa quantitative real-time pcr Radopholus similisis a major pest of East African highland cooking bananas (Musaspp.) in Uganda. Non-pathogenic Fusariumoxysporumendophytes, isolated from bananas in farmers’ fields, have shown potential to reduceR. Similis numbers in tissue culture banana. The mechanism through which endophytes confer resistance to nematodes has previously been demonstrated to involve inducedresistance. In this study, the expression of eight defense-related genes in banana was investigated using quantitative real-time reverse transcription PCR. Plants of susceptible (cv. Nabusa, genomic group AAA-EA) and tolerant (cv. Kayinja, genomic group ABB) banana cultivars were inoculated with endophyticF. Oxysporum strain V5w2. Gene expression levels were analysed following endophyte inoculation and nematode challenge. Endophyte colonization of roots of the tolerant cultivar induced transient expression of POX and suppressed expression ofPR-3,lectin, PAE, PALandPIR7A. CatalaseandPR-1activities were up-regulated in the tolerant cultivar 33days after endophyte colonization of roots, but their expressions were further up-regulated following nematode challenge. Apart from POX andlectin, the other genes analysed were not responsive to endophyte colonization orR. Similis challenge in the susceptible cv.Nabusa. This is the first report of endophyte-induced defense-related gene expression in banana. 2007-10 2018-03-07T11:25:56Z 2018-03-07T11:25:56Z Journal Article https://hdl.handle.net/10568/91450 en Limited Access Elsevier Paparu, P., Dubois, T., Coyne, D. & Viljoen, A. (2007). Defense-related gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with non-pathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis. Physiological and Molecular Plant Pathology, 71(4-6), 149-157. |
| spellingShingle | bananas radopholus similis fungal endophyte fusarium oxysporum gene expression musa quantitative real-time pcr Paparu, P. Dubois, T. Coyne, Danny L. Viljoen, Altus Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis |
| title | Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis |
| title_full | Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis |
| title_fullStr | Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis |
| title_full_unstemmed | Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis |
| title_short | Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis |
| title_sort | defenserelated gene expression in susceptible and tolerant bananas musa spp following inoculation with nonpathogenic fusarium oxysporum endophytes and challenge with radopholus similis |
| topic | bananas radopholus similis fungal endophyte fusarium oxysporum gene expression musa quantitative real-time pcr |
| url | https://hdl.handle.net/10568/91450 |
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