Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria
Xanthomonas axonopodis pv manihotis (Xam) is the causal agent of cassava bacterial blight (CBB) in all cassava growing areas of the world. To develop an efficient disease management strategy, the genetic diversity of the pathogens population ought to be known. Information is scarce on the genetic d...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2006
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| Online Access: | https://hdl.handle.net/10568/91366 |
| _version_ | 1855524841898115072 |
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| author | Ogunjobi, A.A. Fagade, O. Dixon, A. |
| author_browse | Dixon, A. Fagade, O. Ogunjobi, A.A. |
| author_facet | Ogunjobi, A.A. Fagade, O. Dixon, A. |
| author_sort | Ogunjobi, A.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Xanthomonas axonopodis pv manihotis (Xam) is the causal agent of cassava bacterial blight (CBB) in all cassava growing areas of the world. To develop an efficient disease management strategy, the genetic diversity of the pathogens population ought to be known. Information is scarce on the genetic diversity of Xam population in Nigeria. Random amplified polymorphic DNA (RAPD) was used to characterize Xam isolates from the southeastern parts of Nigeria. Seventeen Xam strains and two reference strains were analyzed with four RAPD primers. RAPD fragment data showed five major clusters at 80% similarity coefficient level. Four strains were not clustered by this analysis (Del5A, Imo38B, Ebon28A and Ebon27A) and were not grouped with the others in the dendrogram. Principal component analysis (PCA) assembly grouped the bacterial strains into three. One of the strains that was separated in the dendrogram was also clustered separately from the others in the major cluster in the PCA. The results showed a high level of genetic diversity in the pathogen. |
| format | Journal Article |
| id | CGSpace91366 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| record_format | dspace |
| spelling | CGSpace913662023-02-15T06:54:41Z Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria Ogunjobi, A.A. Fagade, O. Dixon, A. random amplified polymorphic dna genetic variation dendrogram analysis principal component analysis xanthomonas axonopodis pv manihotis cassava bacterial blight pathogens Xanthomonas axonopodis pv manihotis (Xam) is the causal agent of cassava bacterial blight (CBB) in all cassava growing areas of the world. To develop an efficient disease management strategy, the genetic diversity of the pathogens population ought to be known. Information is scarce on the genetic diversity of Xam population in Nigeria. Random amplified polymorphic DNA (RAPD) was used to characterize Xam isolates from the southeastern parts of Nigeria. Seventeen Xam strains and two reference strains were analyzed with four RAPD primers. RAPD fragment data showed five major clusters at 80% similarity coefficient level. Four strains were not clustered by this analysis (Del5A, Imo38B, Ebon28A and Ebon27A) and were not grouped with the others in the dendrogram. Principal component analysis (PCA) assembly grouped the bacterial strains into three. One of the strains that was separated in the dendrogram was also clustered separately from the others in the major cluster in the PCA. The results showed a high level of genetic diversity in the pathogen. 2006 2018-03-07T11:25:43Z 2018-03-07T11:25:43Z Journal Article https://hdl.handle.net/10568/91366 en Open Access Ogunjobi, A.A., Fagade, O. & Dixon, A. (2006). Molecular variation in population structure of Xanthomonas axonopodis pv manihotis in the south eastern Nigeria. African Journal of Biotechnology, 5(20), 1868-1872. |
| spellingShingle | random amplified polymorphic dna genetic variation dendrogram analysis principal component analysis xanthomonas axonopodis pv manihotis cassava bacterial blight pathogens Ogunjobi, A.A. Fagade, O. Dixon, A. Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria |
| title | Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria |
| title_full | Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria |
| title_fullStr | Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria |
| title_full_unstemmed | Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria |
| title_short | Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria |
| title_sort | molecular variation in population structure of xanthomonas axonopodis pv manihotis in the south eastern nigeria |
| topic | random amplified polymorphic dna genetic variation dendrogram analysis principal component analysis xanthomonas axonopodis pv manihotis cassava bacterial blight pathogens |
| url | https://hdl.handle.net/10568/91366 |
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