Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz

Induced mutagenesis holds promise for the subtle manipulation of traits of interest in crop plants. For a vegetatively propagated crop like cassava with severe constraints posed on its genetic improvement by inherent biological systems, the adoption of this methodology seems even the more appealing....

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Autores principales: Owoseni, O., Okwaro, H., Afza, R., Bado, S., Dixon, A., Mba, C.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2006
Materias:
Acceso en línea:https://hdl.handle.net/10568/91347
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author Owoseni, O.
Okwaro, H.
Afza, R.
Bado, S.
Dixon, A.
Mba, C.
author_browse Afza, R.
Bado, S.
Dixon, A.
Mba, C.
Okwaro, H.
Owoseni, O.
author_facet Owoseni, O.
Okwaro, H.
Afza, R.
Bado, S.
Dixon, A.
Mba, C.
author_sort Owoseni, O.
collection Repository of Agricultural Research Outputs (CGSpace)
description Induced mutagenesis holds promise for the subtle manipulation of traits of interest in crop plants. For a vegetatively propagated crop like cassava with severe constraints posed on its genetic improvement by inherent biological systems, the adoption of this methodology seems even the more appealing. However, there is scant information on protocols for inducing mutations in this crop. We present in this report the preliminary data on the determination of radiosensitivities for some African cassava accessions. The optimal doses of gamma ray irradiation varied from as low 12 Gy to 25 Gy. The probable implication of genotypic variation in response to gamma irradiation as was found in this study buttresses the need to carry out this larger scale study in order to avail cassava scientists intending to adopt induced mutagenesis of requisite information in this regard. A modified in vitro culture medium, half strength MS without growth hormones, was also shown to greatly enhance the growth of the plantlets without producing callus.
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spelling CGSpace913472023-06-12T15:40:43Z Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz Owoseni, O. Okwaro, H. Afza, R. Bado, S. Dixon, A. Mba, C. cassava gamma irradiation in vitro mutagenesis genetic improvement genome genotypic variation Induced mutagenesis holds promise for the subtle manipulation of traits of interest in crop plants. For a vegetatively propagated crop like cassava with severe constraints posed on its genetic improvement by inherent biological systems, the adoption of this methodology seems even the more appealing. However, there is scant information on protocols for inducing mutations in this crop. We present in this report the preliminary data on the determination of radiosensitivities for some African cassava accessions. The optimal doses of gamma ray irradiation varied from as low 12 Gy to 25 Gy. The probable implication of genotypic variation in response to gamma irradiation as was found in this study buttresses the need to carry out this larger scale study in order to avail cassava scientists intending to adopt induced mutagenesis of requisite information in this regard. A modified in vitro culture medium, half strength MS without growth hormones, was also shown to greatly enhance the growth of the plantlets without producing callus. 2006 2018-03-07T11:25:40Z 2018-03-07T11:25:40Z Journal Article https://hdl.handle.net/10568/91347 en Limited Access Owoseni, O., Okwaro, H., Afza, R., Bado, S., Dixon, A. & Mba, C. (2006). Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz. Plant Mutation Reports, 1(2), 32-36.
spellingShingle cassava
gamma irradiation
in vitro mutagenesis
genetic improvement
genome
genotypic variation
Owoseni, O.
Okwaro, H.
Afza, R.
Bado, S.
Dixon, A.
Mba, C.
Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz
title Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz
title_full Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz
title_fullStr Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz
title_full_unstemmed Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz
title_short Radiosensitivity and in vitro mutagenesis in African accessions of cassava, Manihot esculenta Crantz
title_sort radiosensitivity and in vitro mutagenesis in african accessions of cassava manihot esculenta crantz
topic cassava
gamma irradiation
in vitro mutagenesis
genetic improvement
genome
genotypic variation
url https://hdl.handle.net/10568/91347
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