Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever
East Coast fever (ECF) is a lymphoproliferative disease caused by the tick-transmitted protozoan parasite Theileria parva. ECF is one of the most serious cattle tick-borne diseases in Sub-Saharan Africa. We have previously demonstrated that three doses of the C-terminal part of the sporozoite protei...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/91022 |
| _version_ | 1855526398406426624 |
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| author | Lacasta, Anna Mwalimu, Stephen Kibwana, Elisabeth Saya, R. Awino, Elias Njoroge, Thomas Poole, Elizabeth J. Ndiwa, Nicholas N. Pelle, Roger Nene, Vishvanath M. Steinaa, Lucilla |
| author_browse | Awino, Elias Kibwana, Elisabeth Lacasta, Anna Mwalimu, Stephen Ndiwa, Nicholas N. Nene, Vishvanath M. Njoroge, Thomas Pelle, Roger Poole, Elizabeth J. Saya, R. Steinaa, Lucilla |
| author_facet | Lacasta, Anna Mwalimu, Stephen Kibwana, Elisabeth Saya, R. Awino, Elias Njoroge, Thomas Poole, Elizabeth J. Ndiwa, Nicholas N. Pelle, Roger Nene, Vishvanath M. Steinaa, Lucilla |
| author_sort | Lacasta, Anna |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | East Coast fever (ECF) is a lymphoproliferative disease caused by the tick-transmitted protozoan parasite Theileria parva. ECF is one of the most serious cattle tick-borne diseases in Sub-Saharan Africa. We have previously demonstrated that three doses of the C-terminal part of the sporozoite protein p67 (p67C) adjuvanted with ISA206VG confers partial protection against ECF at a herd level. We have tested the efficacy of two doses of this experimental vaccine, as reducing the vaccination regimen would facilitate its deployment in the field. We reconfirm that three antigen doses gave a significant level of protection to severe disease (46%, ECF score < 6) when compared with the control group, while two doses did not (23%). Animals receiving three doses of p67C developed higher antibody titers and CD4+ T-cell proliferation indices, than those which received two doses. A new panel of immune parameters were tested in order to identify factors correlating with protection: CD4+ proliferation index, total IgG, IgG1, IgG2 and IgM half maximal titers and neutralization capacity of the sera with and without complement. We show that some of the cellular and humoral immune responses provide preliminary correlates of protection. |
| format | Journal Article |
| id | CGSpace91022 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace910222024-01-17T12:58:34Z Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever Lacasta, Anna Mwalimu, Stephen Kibwana, Elisabeth Saya, R. Awino, Elias Njoroge, Thomas Poole, Elizabeth J. Ndiwa, Nicholas N. Pelle, Roger Nene, Vishvanath M. Steinaa, Lucilla theileria cattle animal diseases vaccines disease control east coast fever East Coast fever (ECF) is a lymphoproliferative disease caused by the tick-transmitted protozoan parasite Theileria parva. ECF is one of the most serious cattle tick-borne diseases in Sub-Saharan Africa. We have previously demonstrated that three doses of the C-terminal part of the sporozoite protein p67 (p67C) adjuvanted with ISA206VG confers partial protection against ECF at a herd level. We have tested the efficacy of two doses of this experimental vaccine, as reducing the vaccination regimen would facilitate its deployment in the field. We reconfirm that three antigen doses gave a significant level of protection to severe disease (46%, ECF score < 6) when compared with the control group, while two doses did not (23%). Animals receiving three doses of p67C developed higher antibody titers and CD4+ T-cell proliferation indices, than those which received two doses. A new panel of immune parameters were tested in order to identify factors correlating with protection: CD4+ proliferation index, total IgG, IgG1, IgG2 and IgM half maximal titers and neutralization capacity of the sera with and without complement. We show that some of the cellular and humoral immune responses provide preliminary correlates of protection. 2018-03 2018-02-19T13:38:28Z 2018-02-19T13:38:28Z Journal Article https://hdl.handle.net/10568/91022 en Open Access Elsevier Lacasta, A., Mwalimu, S., Kibwana, E., Saya, R., Awino, E., Njoroge, T., Poole, J., Ndiwa, N., Pelle, R., Nene, V. and Steinaa, L. 2018. Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever. Vaccine 36(11):1389–1397. |
| spellingShingle | theileria cattle animal diseases vaccines disease control east coast fever Lacasta, Anna Mwalimu, Stephen Kibwana, Elisabeth Saya, R. Awino, Elias Njoroge, Thomas Poole, Elizabeth J. Ndiwa, Nicholas N. Pelle, Roger Nene, Vishvanath M. Steinaa, Lucilla Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever |
| title | Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever |
| title_full | Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever |
| title_fullStr | Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever |
| title_full_unstemmed | Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever |
| title_short | Immune parameters to p67C antigen adjuvanted with ISA206VG correlate with protection against East Coast fever |
| title_sort | immune parameters to p67c antigen adjuvanted with isa206vg correlate with protection against east coast fever |
| topic | theileria cattle animal diseases vaccines disease control east coast fever |
| url | https://hdl.handle.net/10568/91022 |
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