Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci
An infection and treatment protocol involving infection with a mixture of three parasite isolates and simultaneous treatment with oxytetracycline is currently used to vaccinate cattle against Theileria parva. While vaccination results in high levels of protection in some regions, little or no protec...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/90996 |
| _version_ | 1855514293050540032 |
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| author | Hemmink, Johanneke D. Sitt, Tatjana Pelle, Roger Klerk-Lorist, L.M. de Shiels, B. Toye, Philip G. Morrison, W.I. Weir, W. |
| author_browse | Hemmink, Johanneke D. Klerk-Lorist, L.M. de Morrison, W.I. Pelle, Roger Shiels, B. Sitt, Tatjana Toye, Philip G. Weir, W. |
| author_facet | Hemmink, Johanneke D. Sitt, Tatjana Pelle, Roger Klerk-Lorist, L.M. de Shiels, B. Toye, Philip G. Morrison, W.I. Weir, W. |
| author_sort | Hemmink, Johanneke D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | An infection and treatment protocol involving infection with a mixture of three parasite isolates and simultaneous treatment with oxytetracycline is currently used to vaccinate cattle against Theileria parva. While vaccination results in high levels of protection in some regions, little or no protection is observed in areas where animals are challenged predominantly by parasites of buffalo origin. A previous study involving sequencing of two antigen-encoding genes from a series of parasite isolates indicated that this is associated with greater antigenic diversity in buffalo-derived T. parva. The current study set out to extend these analyses by applying high-throughput sequencing to ex vivo samples from naturally infected buffalo to determine the extent of diversity in a set of antigen-encoding genes. Samples from two populations of buffalo, one in Kenya and the other in South Africa, were examined to investigate the effect of geographical distance on the nature of sequence diversity. The results revealed a number of significant findings. First, there was a variable degree of nucleotide sequence diversity in all gene segments examined, with the percentage of polymorphic nucleotides ranging from 10% to 69%. Second, large numbers of allelic variants of each gene were found in individual animals, indicating multiple infection events. Third, despite the observed diversity in nucleotide sequences, several of the gene products had highly conserved amino acid sequences, and thus represent potential candidates for vaccine development. Fourth, although compelling evidence for population differentiation between the Kenyan and South African T. parva parasites was identified, analysis of molecular variance for each gene revealed that the majority of the underlying nucleotide sequence polymorphism was common to both areas, indicating that much of this aspect of genetic variation in the parasite population arose prior to geographic separation. |
| format | Journal Article |
| id | CGSpace90996 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace909962023-12-08T19:36:04Z Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci Hemmink, Johanneke D. Sitt, Tatjana Pelle, Roger Klerk-Lorist, L.M. de Shiels, B. Toye, Philip G. Morrison, W.I. Weir, W. african buffaloes animal diseases vaccines infectious diseases parasitology An infection and treatment protocol involving infection with a mixture of three parasite isolates and simultaneous treatment with oxytetracycline is currently used to vaccinate cattle against Theileria parva. While vaccination results in high levels of protection in some regions, little or no protection is observed in areas where animals are challenged predominantly by parasites of buffalo origin. A previous study involving sequencing of two antigen-encoding genes from a series of parasite isolates indicated that this is associated with greater antigenic diversity in buffalo-derived T. parva. The current study set out to extend these analyses by applying high-throughput sequencing to ex vivo samples from naturally infected buffalo to determine the extent of diversity in a set of antigen-encoding genes. Samples from two populations of buffalo, one in Kenya and the other in South Africa, were examined to investigate the effect of geographical distance on the nature of sequence diversity. The results revealed a number of significant findings. First, there was a variable degree of nucleotide sequence diversity in all gene segments examined, with the percentage of polymorphic nucleotides ranging from 10% to 69%. Second, large numbers of allelic variants of each gene were found in individual animals, indicating multiple infection events. Third, despite the observed diversity in nucleotide sequences, several of the gene products had highly conserved amino acid sequences, and thus represent potential candidates for vaccine development. Fourth, although compelling evidence for population differentiation between the Kenyan and South African T. parva parasites was identified, analysis of molecular variance for each gene revealed that the majority of the underlying nucleotide sequence polymorphism was common to both areas, indicating that much of this aspect of genetic variation in the parasite population arose prior to geographic separation. 2018-03 2018-02-15T09:38:12Z 2018-02-15T09:38:12Z Journal Article https://hdl.handle.net/10568/90996 en Open Access Elsevier Hemmink, J.D., Sitt, T., Pelle, R., Klerk-Lorist, L.-M. de, Shiels, B., Toye, P.G., Morrison, W.I. and Weir, W. 2018. Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci. International Journal for Parasitology 48(3–4):287-296. |
| spellingShingle | african buffaloes animal diseases vaccines infectious diseases parasitology Hemmink, Johanneke D. Sitt, Tatjana Pelle, Roger Klerk-Lorist, L.M. de Shiels, B. Toye, Philip G. Morrison, W.I. Weir, W. Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci |
| title | Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci |
| title_full | Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci |
| title_fullStr | Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci |
| title_full_unstemmed | Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci |
| title_short | Ancient diversity and geographical sub-structuring in African buffalo Theileria parva populations revealed through metagenetic analysis of antigen-encoding loci |
| title_sort | ancient diversity and geographical sub structuring in african buffalo theileria parva populations revealed through metagenetic analysis of antigen encoding loci |
| topic | african buffaloes animal diseases vaccines infectious diseases parasitology |
| url | https://hdl.handle.net/10568/90996 |
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