Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L.
In vitro culture media capable of regenerating moderate to high shoots and/or plantlets from meristems of two yam species – Dioscorea rotundata and Dioscorea alata within comparable duration of 10 weeks as commonly obtained in other monocots and root and tuber crops were investigated. The study comp...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/90907 |
| _version_ | 1855540647182729216 |
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| author | Adeniyi, O.J. Adetimirin, Victor O. Ingelbrecht, I.L. Asiedu, Robert |
| author_browse | Adeniyi, O.J. Adetimirin, Victor O. Asiedu, Robert Ingelbrecht, I.L. |
| author_facet | Adeniyi, O.J. Adetimirin, Victor O. Ingelbrecht, I.L. Asiedu, Robert |
| author_sort | Adeniyi, O.J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In vitro culture media capable of regenerating moderate to high shoots and/or plantlets from meristems
of two yam species – Dioscorea rotundata and Dioscorea alata within comparable duration of 10 weeks
as commonly obtained in other monocots and root and tuber crops were investigated. The study
comprised 125 phytohormone combinations investigated in three factorial experiments each consisting
of an auxin (NAA) and a cytokinin (BAP or kinetin), or two cytokinins only. The frequency of direct
plantlet regeneration, though significantly (P < 0.05) higher for D. alata than for D. rotundata, was low
and ranged from 0 to 10% at 3 weeks after culture (WAC) and 0 to 35% at 8 WAC. At 8 WAC, shoot
regeneration of 42-75% was obtained in D. rotundata in MS medium supplemented with 0.1 M NAA +
0.20 M BAP, and shoot + plantlet regeneration of 60-82% obtained in media containing 0.05 M + 0.20
M BAP or 0.46 M BAP + 0.50 M kinetin in D. alata. Both shoot induction and plantlet regeneration
were species-dependent. Induced shoots were successfully rooted in MS medium within 3 to 4 weeks,
bringing time taken for plantlet regeneration to 11 to 12 weeks. Regenerants were morphologically
similar to the mother plants. Results of the present study will facilitate regeneration of plantlets via
meristem in D. rotundata and D. alata. |
| format | Journal Article |
| id | CGSpace90907 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| record_format | dspace |
| spelling | CGSpace909072025-11-11T11:04:37Z Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L. Adeniyi, O.J. Adetimirin, Victor O. Ingelbrecht, I.L. Asiedu, Robert meristems phytohormone combinations plantlets shoots yams dioscorea rotundata yam mosaic virus germplasm In vitro culture media capable of regenerating moderate to high shoots and/or plantlets from meristems of two yam species – Dioscorea rotundata and Dioscorea alata within comparable duration of 10 weeks as commonly obtained in other monocots and root and tuber crops were investigated. The study comprised 125 phytohormone combinations investigated in three factorial experiments each consisting of an auxin (NAA) and a cytokinin (BAP or kinetin), or two cytokinins only. The frequency of direct plantlet regeneration, though significantly (P < 0.05) higher for D. alata than for D. rotundata, was low and ranged from 0 to 10% at 3 weeks after culture (WAC) and 0 to 35% at 8 WAC. At 8 WAC, shoot regeneration of 42-75% was obtained in D. rotundata in MS medium supplemented with 0.1 M NAA + 0.20 M BAP, and shoot + plantlet regeneration of 60-82% obtained in media containing 0.05 M + 0.20 M BAP or 0.46 M BAP + 0.50 M kinetin in D. alata. Both shoot induction and plantlet regeneration were species-dependent. Induced shoots were successfully rooted in MS medium within 3 to 4 weeks, bringing time taken for plantlet regeneration to 11 to 12 weeks. Regenerants were morphologically similar to the mother plants. Results of the present study will facilitate regeneration of plantlets via meristem in D. rotundata and D. alata. 2008-04 2018-02-06T12:15:20Z 2018-02-06T12:15:20Z Journal Article https://hdl.handle.net/10568/90907 en Open Access application/pdf Adeniyi, O.J., Adetimirin, V.O., Ingelbrecht, I. & Asiedu, R. (2008). Shoot and plantlet regeneration from meristems of Dioscorea rotundata Poir and Dioscorea alata L. African Journal of Biotechnology, 7(8), 1003-1008. |
| spellingShingle | meristems phytohormone combinations plantlets shoots yams dioscorea rotundata yam mosaic virus germplasm Adeniyi, O.J. Adetimirin, Victor O. Ingelbrecht, I.L. Asiedu, Robert Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L. |
| title | Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L. |
| title_full | Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L. |
| title_fullStr | Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L. |
| title_full_unstemmed | Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L. |
| title_short | Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L. |
| title_sort | shoot and plantlet regeneration from meristems of dioscorea rotundata poir and dioscorea alata l |
| topic | meristems phytohormone combinations plantlets shoots yams dioscorea rotundata yam mosaic virus germplasm |
| url | https://hdl.handle.net/10568/90907 |
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