Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants
The experiment was conducted to test the in vitro regeneration efficiency of cowpea shoots via embryonic axes explants and the effect of benzyl amino purine (BAP) concentration on shoot formation. A total of 600 explants (embryonic axes) were plated from which 237 shoots were regenerated. The averag...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2008
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/90893 |
| _version_ | 1855531454979637248 |
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| author | Yusuf, M. Raji, A.A. Ingelbrecht, I.L. Katung, M.D. |
| author_browse | Ingelbrecht, I.L. Katung, M.D. Raji, A.A. Yusuf, M. |
| author_facet | Yusuf, M. Raji, A.A. Ingelbrecht, I.L. Katung, M.D. |
| author_sort | Yusuf, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The experiment was conducted to test the in vitro regeneration efficiency of cowpea shoots via
embryonic axes explants and the effect of benzyl amino purine (BAP) concentration on shoot formation.
A total of 600 explants (embryonic axes) were plated from which 237 shoots were regenerated. The
average regeneration ratio was 39% and the number of shoot per explant obtained stood at an average
of 6.0. This indicates likelihood that higher regeneration percent and number of shoots per explant
could be possible as more proficiency is attained in the use of the protocol. The role of the growth
regulator, benzyl amino purine (BAP), as a shoot growth inducing factor and modifier in cowpea in vitro
regeneration was demonstrated in the experiment. The protocol used in the experiment gave promising
results for the efficient regeneration and subsequent genetic transformation of cowpea. |
| format | Journal Article |
| id | CGSpace90893 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| record_format | dspace |
| spelling | CGSpace908932025-11-11T10:36:32Z Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants Yusuf, M. Raji, A.A. Ingelbrecht, I.L. Katung, M.D. cowpeas in vitro regeneration ratio benzyl amino purine (bap) embryonic axes explants genetic transformation of cowpea biotechnology The experiment was conducted to test the in vitro regeneration efficiency of cowpea shoots via embryonic axes explants and the effect of benzyl amino purine (BAP) concentration on shoot formation. A total of 600 explants (embryonic axes) were plated from which 237 shoots were regenerated. The average regeneration ratio was 39% and the number of shoot per explant obtained stood at an average of 6.0. This indicates likelihood that higher regeneration percent and number of shoots per explant could be possible as more proficiency is attained in the use of the protocol. The role of the growth regulator, benzyl amino purine (BAP), as a shoot growth inducing factor and modifier in cowpea in vitro regeneration was demonstrated in the experiment. The protocol used in the experiment gave promising results for the efficient regeneration and subsequent genetic transformation of cowpea. 2008 2018-02-06T12:15:17Z 2018-02-06T12:15:17Z Journal Article https://hdl.handle.net/10568/90893 en Open Access application/pdf Yusuf, M., Raji, A.A., Ingelbrecht, I. & Katung, M.D. (2008). Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants. African Journal of Plant Science, 2(9), 105-108. |
| spellingShingle | cowpeas in vitro regeneration ratio benzyl amino purine (bap) embryonic axes explants genetic transformation of cowpea biotechnology Yusuf, M. Raji, A.A. Ingelbrecht, I.L. Katung, M.D. Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants |
| title | Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants |
| title_full | Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants |
| title_fullStr | Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants |
| title_full_unstemmed | Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants |
| title_short | Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants |
| title_sort | regeneration efficiency of cowpea vigna unguiculata l walp via embryonic axes explants |
| topic | cowpeas in vitro regeneration ratio benzyl amino purine (bap) embryonic axes explants genetic transformation of cowpea biotechnology |
| url | https://hdl.handle.net/10568/90893 |
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