Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues
East Africa is the largest banana producing and consuming region in Africa. In particular, the East African Highland Banana serves as the major staple crop of countries like Uganda, but production is constrained by a number of serious pests and diseases. Banana breeding is a very difficult and slow...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/90891 |
| _version_ | 1855534056755691520 |
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| author | Tripathi, L. Tripathi, J.N. Tushemereirwe, Wilberforce K. |
| author_browse | Tripathi, J.N. Tripathi, L. Tushemereirwe, Wilberforce K. |
| author_facet | Tripathi, L. Tripathi, J.N. Tushemereirwe, Wilberforce K. |
| author_sort | Tripathi, L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | East Africa is the largest banana producing and consuming region in Africa. In particular, the East
African Highland Banana serves as the major staple crop of countries like Uganda, but production is
constrained by a number of serious pests and diseases. Banana breeding is a very difficult and slow
process, so genetic engineering offers an alternative approach to improvement. A transformation
system using intercalary meristematic tissues was developed using Agrobacterium strain EHA105
harboring the binary vector pCAMBIA2301 containing the gusA reporter gene and nptII as selectable
marker. In this paper, a new transformation protocol is described that yields kanamycin-resistant, GUSexpressing
banana plants from roughly 10% of the initial explants. The resulting fully-rooted transgenic
plants do not appear to be chimeras since they can be stably propagated, GUS activity is observed
uniformly throughout the plants including the germline cells of the meristem, and PCR and Southern
blots indicate stable integration of the genes into the genome. |
| format | Journal Article |
| id | CGSpace90891 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| record_format | dspace |
| spelling | CGSpace908912025-11-11T10:39:52Z Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues Tripathi, L. Tripathi, J.N. Tushemereirwe, Wilberforce K. agrobacterium intercalary meristem genetic transformation bananas east african highland bananas xanthomonas banana streak virus East Africa is the largest banana producing and consuming region in Africa. In particular, the East African Highland Banana serves as the major staple crop of countries like Uganda, but production is constrained by a number of serious pests and diseases. Banana breeding is a very difficult and slow process, so genetic engineering offers an alternative approach to improvement. A transformation system using intercalary meristematic tissues was developed using Agrobacterium strain EHA105 harboring the binary vector pCAMBIA2301 containing the gusA reporter gene and nptII as selectable marker. In this paper, a new transformation protocol is described that yields kanamycin-resistant, GUSexpressing banana plants from roughly 10% of the initial explants. The resulting fully-rooted transgenic plants do not appear to be chimeras since they can be stably propagated, GUS activity is observed uniformly throughout the plants including the germline cells of the meristem, and PCR and Southern blots indicate stable integration of the genes into the genome. 2008-05 2018-02-06T12:15:16Z 2018-02-06T12:15:16Z Journal Article https://hdl.handle.net/10568/90891 en Open Access application/pdf Tripathi, L., Tripathi, J.N. & Tushemereirwe, W.K. (2008). Rapid and efficient production of transgenic East African Highland Banana (Musa spp.) using intercalary meristematic tissues. African Journal of Biotechnology, 7(10), 1438-1445. |
| spellingShingle | agrobacterium intercalary meristem genetic transformation bananas east african highland bananas xanthomonas banana streak virus Tripathi, L. Tripathi, J.N. Tushemereirwe, Wilberforce K. Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues |
| title | Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues |
| title_full | Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues |
| title_fullStr | Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues |
| title_full_unstemmed | Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues |
| title_short | Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues |
| title_sort | rapid and efficient production of transgenic east african highland banana musa spp using intercalary meristematic tissues |
| topic | agrobacterium intercalary meristem genetic transformation bananas east african highland bananas xanthomonas banana streak virus |
| url | https://hdl.handle.net/10568/90891 |
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