High frequency shoot regeneration of various cultivars of banana (Musa sp.)
An efficient, simple, and rapid regeneration system was established for bananas (Musa sp.) using sections of corm containing intercalary meristematic tissues as explants. Six different cultivars of banana (‘Mpologoma’, ‘Nakitembe’, ‘Mbwazirume’, ‘Pisang awak’, ‘Sukali ndiizi’, and ‘FHIA-17’) with di...
| Main Authors: | , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Informa UK Limited
2008
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/90831 |
| _version_ | 1855525578248028160 |
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| author | Tripathi, L. Tripathi, J.N. |
| author_browse | Tripathi, J.N. Tripathi, L. |
| author_facet | Tripathi, L. Tripathi, J.N. |
| author_sort | Tripathi, L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | An efficient, simple, and rapid regeneration system was
established for bananas (Musa sp.) using sections of corm containing intercalary
meristematic tissues as explants. Six different cultivars of banana
(‘Mpologoma’, ‘Nakitembe’, ‘Mbwazirume’, ‘Pisang awak’, ‘Sukali
ndiizi’, and ‘FHIA-17’) with diverse genetic constitution and ploidy levels
were regenerated on Murashige and Skoog (MS) medium supplemented
with 5 mgl−1 of 6-benzylaminopurine. All the cultivars showed relatively
high regeneration efficiency. About 93–97% of the explants regenerated,
producing, on average, 12–13 shoots from whole section and 16–19 shoots
in total from quarter pieces of each section. Regeneration from intercalary
meristematic tissues offers a simple, efficient method for transformation of
a broad range of banana cultivars, including East African Highland
bananas.
KEYWOR |
| format | Journal Article |
| id | CGSpace90831 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace908312024-05-15T05:11:41Z High frequency shoot regeneration of various cultivars of banana (Musa sp.) Tripathi, L. Tripathi, J.N. bananas corm sections intercalary meristematic tissues regeneration tropical and subtropical fusarium oxysporum regeneration system An efficient, simple, and rapid regeneration system was established for bananas (Musa sp.) using sections of corm containing intercalary meristematic tissues as explants. Six different cultivars of banana (‘Mpologoma’, ‘Nakitembe’, ‘Mbwazirume’, ‘Pisang awak’, ‘Sukali ndiizi’, and ‘FHIA-17’) with diverse genetic constitution and ploidy levels were regenerated on Murashige and Skoog (MS) medium supplemented with 5 mgl−1 of 6-benzylaminopurine. All the cultivars showed relatively high regeneration efficiency. About 93–97% of the explants regenerated, producing, on average, 12–13 shoots from whole section and 16–19 shoots in total from quarter pieces of each section. Regeneration from intercalary meristematic tissues offers a simple, efficient method for transformation of a broad range of banana cultivars, including East African Highland bananas. KEYWOR 2008-08-19 2018-02-06T12:14:58Z 2018-02-06T12:14:58Z Journal Article https://hdl.handle.net/10568/90831 en Limited Access Informa UK Limited Tripathi, L. & Tripathi, J.N. (2008). High-Frequency Shoot Regeneration of Various Cultivars of Banana (Musa sp.). Journal of Crop Improvement, 22(2), 171-180. |
| spellingShingle | bananas corm sections intercalary meristematic tissues regeneration tropical and subtropical fusarium oxysporum regeneration system Tripathi, L. Tripathi, J.N. High frequency shoot regeneration of various cultivars of banana (Musa sp.) |
| title | High frequency shoot regeneration of various cultivars of banana (Musa sp.) |
| title_full | High frequency shoot regeneration of various cultivars of banana (Musa sp.) |
| title_fullStr | High frequency shoot regeneration of various cultivars of banana (Musa sp.) |
| title_full_unstemmed | High frequency shoot regeneration of various cultivars of banana (Musa sp.) |
| title_short | High frequency shoot regeneration of various cultivars of banana (Musa sp.) |
| title_sort | high frequency shoot regeneration of various cultivars of banana musa sp |
| topic | bananas corm sections intercalary meristematic tissues regeneration tropical and subtropical fusarium oxysporum regeneration system |
| url | https://hdl.handle.net/10568/90831 |
| work_keys_str_mv | AT tripathil highfrequencyshootregenerationofvariouscultivarsofbananamusasp AT tripathijn highfrequencyshootregenerationofvariouscultivarsofbananamusasp |