A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction
For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate University....
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2009
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/90739 |
| _version_ | 1855538493954981888 |
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| author | Narina, S.S. Mohammed, I. Asiedu, Robert Mignouna, Hodeba D. |
| author_browse | Asiedu, Robert Mignouna, Hodeba D. Mohammed, I. Narina, S.S. |
| author_facet | Narina, S.S. Mohammed, I. Asiedu, Robert Mignouna, Hodeba D. |
| author_sort | Narina, S.S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate University. The RNA isolated using this standardized protocol was high in quality and led to successful good quality cDNA library construction and identification of functional ESTs in yam. |
| format | Journal Article |
| id | CGSpace90739 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| record_format | dspace |
| spelling | CGSpace907392024-03-06T10:16:43Z A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction Narina, S.S. Mohammed, I. Asiedu, Robert Mignouna, Hodeba D. yams genotypes colletotrichum gloeosporioides cdna rna diseases For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate University. The RNA isolated using this standardized protocol was high in quality and led to successful good quality cDNA library construction and identification of functional ESTs in yam. 2009 2018-02-06T12:14:33Z 2018-02-06T12:14:33Z Journal Article https://hdl.handle.net/10568/90739 en Limited Access Narina, S.S., Mohamed, A.I., Asiedu, R. & Mignouna, H.D. (2009). A Standardized RNA Isolation Protocol for Yam (Dioscorea alata L) cDNA Library Construction. Virginia Journal of Science, 60(4), 171-180. |
| spellingShingle | yams genotypes colletotrichum gloeosporioides cdna rna diseases Narina, S.S. Mohammed, I. Asiedu, Robert Mignouna, Hodeba D. A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction |
| title | A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction |
| title_full | A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction |
| title_fullStr | A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction |
| title_full_unstemmed | A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction |
| title_short | A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction |
| title_sort | standardized rna isolation protocol for yam dioscorea alata l cdna library construction |
| topic | yams genotypes colletotrichum gloeosporioides cdna rna diseases |
| url | https://hdl.handle.net/10568/90739 |
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