Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster
Infections caused by Mycoplasma species belonging to the ‘mycoides cluster’ negatively affect the agricultural sector through losses in livestock productivity. These Mycoplasma strains are resistant to many conventional antibiotics due to the total lack of cell wall. Therefore, there is an urgent ne...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2017
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/90646 |
| _version_ | 1855534346567417856 |
|---|---|
| author | Kama-Kama, F. Omosa, L.K. Ng'ang'a, J. Maina, N. Osanjo, G. Yaouba, Souaibou Ilias, M. Midiwo, Jacob Naessens, Jan |
| author_browse | Ilias, M. Kama-Kama, F. Maina, N. Midiwo, Jacob Naessens, Jan Ng'ang'a, J. Omosa, L.K. Osanjo, G. Yaouba, Souaibou |
| author_facet | Kama-Kama, F. Omosa, L.K. Ng'ang'a, J. Maina, N. Osanjo, G. Yaouba, Souaibou Ilias, M. Midiwo, Jacob Naessens, Jan |
| author_sort | Kama-Kama, F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Infections caused by Mycoplasma species belonging to the ‘mycoides cluster’ negatively affect the agricultural sector through losses in livestock productivity. These Mycoplasma strains are resistant to many conventional antibiotics due to the total lack of cell wall. Therefore, there is an urgent need to develop new antimicrobial agents from alternative sources such as medicinal plants to curb the resistance threat. Recent studies on extracts from Solanum aculeastrum and Piliostigma thonningii revealed interesting antimycoplasmal activities hence the motivation to investigate the antimycoplasmal activities of constituent compounds. The CH2Cl2/MeOH extracts from the berries of S. aculeastrum yielded a new β-sitosterol derivative (1) along with six known ones including; lupeol (2), two long-chain fatty alcohols namely undecyl alcohol (3) and lauryl alcohol (4); two long-chain fatty acids namely; myristic acid (5) and nervonic acid (6) as well as a glycosidic steroidal alkaloid; (25R)-3β-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranosyloxy-22α-N-spirosol-5-ene (7) from the MeOH extracts. A new furan diglycoside, (2,5-D-diglucopyranosyloxy-furan) (8) was also characterized from the CH2Cl2/MeOH extract of stem bark of P. thonningii. The structures of the compounds were determined on the basis of spectroscopic evidence and comparison with literature data. Compounds 1, 3, 4, 7, and 8 isolated in sufficient yields were tested against the growth of two Mycoplasma mycoides subsp. mycoides(Mmm), two M. mycoides. capri (Mmc), and one M. capricolum capricolum (Mcc) using broth dilution methods, while the minimum inhibitory concentration (MIC) was determined by serial dilution. The inhibition of Mycoplasma in vitro growth was determined by the use of both flow cytometry (FCM) and color change units (CCU) methods. Compounds 4 and 7showed moderate activity against the growth of Mmm and Mmc but were inactive against the growth of Mcc. The lowest MIC value was 50 μg/ml for compound 7 against Mmm. The rest of the compounds showed minimal or no activity against the strains of Mycoplasma mycoides tested. This is the first report on the use of combined FCM and CCU to determine inhibition of in vitro growth of Mycoplasma mycoides. The activity of these compounds against other bacterial strains should be tested and their safety profiles determined. |
| format | Journal Article |
| id | CGSpace90646 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace906462024-05-01T08:18:47Z Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster Kama-Kama, F. Omosa, L.K. Ng'ang'a, J. Maina, N. Osanjo, G. Yaouba, Souaibou Ilias, M. Midiwo, Jacob Naessens, Jan animal diseases disease control Infections caused by Mycoplasma species belonging to the ‘mycoides cluster’ negatively affect the agricultural sector through losses in livestock productivity. These Mycoplasma strains are resistant to many conventional antibiotics due to the total lack of cell wall. Therefore, there is an urgent need to develop new antimicrobial agents from alternative sources such as medicinal plants to curb the resistance threat. Recent studies on extracts from Solanum aculeastrum and Piliostigma thonningii revealed interesting antimycoplasmal activities hence the motivation to investigate the antimycoplasmal activities of constituent compounds. The CH2Cl2/MeOH extracts from the berries of S. aculeastrum yielded a new β-sitosterol derivative (1) along with six known ones including; lupeol (2), two long-chain fatty alcohols namely undecyl alcohol (3) and lauryl alcohol (4); two long-chain fatty acids namely; myristic acid (5) and nervonic acid (6) as well as a glycosidic steroidal alkaloid; (25R)-3β-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranosyloxy-22α-N-spirosol-5-ene (7) from the MeOH extracts. A new furan diglycoside, (2,5-D-diglucopyranosyloxy-furan) (8) was also characterized from the CH2Cl2/MeOH extract of stem bark of P. thonningii. The structures of the compounds were determined on the basis of spectroscopic evidence and comparison with literature data. Compounds 1, 3, 4, 7, and 8 isolated in sufficient yields were tested against the growth of two Mycoplasma mycoides subsp. mycoides(Mmm), two M. mycoides. capri (Mmc), and one M. capricolum capricolum (Mcc) using broth dilution methods, while the minimum inhibitory concentration (MIC) was determined by serial dilution. The inhibition of Mycoplasma in vitro growth was determined by the use of both flow cytometry (FCM) and color change units (CCU) methods. Compounds 4 and 7showed moderate activity against the growth of Mmm and Mmc but were inactive against the growth of Mcc. The lowest MIC value was 50 μg/ml for compound 7 against Mmm. The rest of the compounds showed minimal or no activity against the strains of Mycoplasma mycoides tested. This is the first report on the use of combined FCM and CCU to determine inhibition of in vitro growth of Mycoplasma mycoides. The activity of these compounds against other bacterial strains should be tested and their safety profiles determined. 2017-12-21 2018-01-30T09:23:24Z 2018-01-30T09:23:24Z Journal Article https://hdl.handle.net/10568/90646 en Open Access Frontiers Media Kama-Kama, F., Omosa, L.K., Nganga, J., Maina, N., Osanjo, G., Yaouba, S., Ilias, M., Midiwo, J. and Naessens, J. 2017. Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster. Frontiers in pharmacology 8:920. |
| spellingShingle | animal diseases disease control Kama-Kama, F. Omosa, L.K. Ng'ang'a, J. Maina, N. Osanjo, G. Yaouba, Souaibou Ilias, M. Midiwo, Jacob Naessens, Jan Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster |
| title | Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster |
| title_full | Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster |
| title_fullStr | Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster |
| title_full_unstemmed | Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster |
| title_short | Antimycoplasmal Activities of Compounds from Solanum aculeastrum and Piliostigma thonningii against Strains from the Mycoplasma mycoides Cluster |
| title_sort | antimycoplasmal activities of compounds from solanum aculeastrum and piliostigma thonningii against strains from the mycoplasma mycoides cluster |
| topic | animal diseases disease control |
| url | https://hdl.handle.net/10568/90646 |
| work_keys_str_mv | AT kamakamaf antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster AT omosalk antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster AT ngangaj antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster AT mainan antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster AT osanjog antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster AT yaoubasouaibou antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster AT iliasm antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster AT midiwojacob antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster AT naessensjan antimycoplasmalactivitiesofcompoundsfromsolanumaculeastrumandpiliostigmathonningiiagainststrainsfromthemycoplasmamycoidescluster |