First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa

Abstract: Group C rotavirus (RVC) has been described to be a causative agent of gastroenteritis in humans and animals including pigs, cows, and dogs. Fecal samples collected from asymptomatic pigs in smallholder swine farms in Kenya and Uganda were screened for the presence of group C rotaviruses (R...

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Autores principales: Amimo, Joshua O., Machuka, Eunice M., Okoth, Edward A.
Formato: Journal Article
Lenguaje:Inglés
Publicado: MDPI 2017
Materias:
Acceso en línea:https://hdl.handle.net/10568/90509
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author Amimo, Joshua O.
Machuka, Eunice M.
Okoth, Edward A.
author_browse Amimo, Joshua O.
Machuka, Eunice M.
Okoth, Edward A.
author_facet Amimo, Joshua O.
Machuka, Eunice M.
Okoth, Edward A.
author_sort Amimo, Joshua O.
collection Repository of Agricultural Research Outputs (CGSpace)
description Abstract: Group C rotavirus (RVC) has been described to be a causative agent of gastroenteritis in humans and animals including pigs, cows, and dogs. Fecal samples collected from asymptomatic pigs in smallholder swine farms in Kenya and Uganda were screened for the presence of group C rotaviruses (RVC) using a reverse transcription-polymerase chain reaction assay. A total of 446 samples were tested and 37 were positive (8.3%). A significantly larger (p < 0.05) number of RVC-positive samples was detected in groups of older pigs (5–6 months) than in younger piglets (1–2 months). There were no significant differences in the RVC detection rate between the pigs that were full time housed/tethered and those that were free range combined with housing/tethering. After compiling these data with diagnostic results for group A rotaviruses (RVA), 13 RVC-positive samples were also positive for RVA. This study provides the first evidence that porcine group C rotavirus may be detected frequently in asymptomatic piglets (aged < 1–6 months) in East Africa. The occurrence of RVC in mixed infections with RVA and other enteric pathogens requires further research to investigate the pathogenic potential of RVC in pigs.
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spelling CGSpace905092025-12-02T10:59:51Z First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa Amimo, Joshua O. Machuka, Eunice M. Okoth, Edward A. swine smallholders infectious diseases molecular biology Abstract: Group C rotavirus (RVC) has been described to be a causative agent of gastroenteritis in humans and animals including pigs, cows, and dogs. Fecal samples collected from asymptomatic pigs in smallholder swine farms in Kenya and Uganda were screened for the presence of group C rotaviruses (RVC) using a reverse transcription-polymerase chain reaction assay. A total of 446 samples were tested and 37 were positive (8.3%). A significantly larger (p < 0.05) number of RVC-positive samples was detected in groups of older pigs (5–6 months) than in younger piglets (1–2 months). There were no significant differences in the RVC detection rate between the pigs that were full time housed/tethered and those that were free range combined with housing/tethering. After compiling these data with diagnostic results for group A rotaviruses (RVA), 13 RVC-positive samples were also positive for RVA. This study provides the first evidence that porcine group C rotavirus may be detected frequently in asymptomatic piglets (aged < 1–6 months) in East Africa. The occurrence of RVC in mixed infections with RVA and other enteric pathogens requires further research to investigate the pathogenic potential of RVC in pigs. 2017 2018-01-17T15:17:18Z 2018-01-17T15:17:18Z Journal Article https://hdl.handle.net/10568/90509 en Open Access MDPI Amimo, J.O., Machuka, E.M. and Okoth, E. 2017. First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa. Pathogens 6(3): 37.
spellingShingle swine
smallholders
infectious diseases
molecular biology
Amimo, Joshua O.
Machuka, Eunice M.
Okoth, Edward A.
First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa
title First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa
title_full First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa
title_fullStr First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa
title_full_unstemmed First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa
title_short First detection of rotavirus group C in asymptomatic pigs of smallholder farms in East Africa
title_sort first detection of rotavirus group c in asymptomatic pigs of smallholder farms in east africa
topic swine
smallholders
infectious diseases
molecular biology
url https://hdl.handle.net/10568/90509
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