Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats

A collection of 89 water yam (Dioscorea alata L.) accessions from Benin, Congo, Côte d’Ivoire, Equatorial Guinea, Gabon, Ghana, Nigeria, Sierra Leone and Togo was assessed for genetic diversity using thirteen microsatellite loci. These 89 are some of the D. alata accessions conserved by the Interna...

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Autores principales: Obidiegwu, J.E., Asiedu, Robert, Ene-Obong, E.E., Muoneke, C.O., Kolesnikova-Allen, M.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2010
Materias:
Acceso en línea:https://hdl.handle.net/10568/90348
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author Obidiegwu, J.E.
Asiedu, Robert
Ene-Obong, E.E.
Muoneke, C.O.
Kolesnikova-Allen, M.
author_browse Asiedu, Robert
Ene-Obong, E.E.
Kolesnikova-Allen, M.
Muoneke, C.O.
Obidiegwu, J.E.
author_facet Obidiegwu, J.E.
Asiedu, Robert
Ene-Obong, E.E.
Muoneke, C.O.
Kolesnikova-Allen, M.
author_sort Obidiegwu, J.E.
collection Repository of Agricultural Research Outputs (CGSpace)
description A collection of 89 water yam (Dioscorea alata L.) accessions from Benin, Congo, Côte d’Ivoire, Equatorial Guinea, Gabon, Ghana, Nigeria, Sierra Leone and Togo was assessed for genetic diversity using thirteen microsatellite loci. These 89 are some of the D. alata accessions conserved by the International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria. A total of 97 alleles were detected with an average allele number of 7.46 per locus. Polymorphism information content (PIC) mean value of 0.65 showed existence of variability among the accessions. Accessions from Nigeria showed highest gene diversity of 0.678 while those from Côte d’Ivoire had lowest diversity with 0.596. Observed mean heterozygosity value of 0.469 was observed. Cluster and principal coordinate analysis showed 8 major cluster groups. There was no relationship between relatedness of the accessions and their geographical area of collection. SSR markers proved to be effective to characterise studied D. alata germplasm.
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spelling CGSpace903482023-02-15T07:15:52Z Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats Obidiegwu, J.E. Asiedu, Robert Ene-Obong, E.E. Muoneke, C.O. Kolesnikova-Allen, M. core germplasm dioscorea alata genetic variation microsatellite yams electrophoresis heterozygosity polymorphic A collection of 89 water yam (Dioscorea alata L.) accessions from Benin, Congo, Côte d’Ivoire, Equatorial Guinea, Gabon, Ghana, Nigeria, Sierra Leone and Togo was assessed for genetic diversity using thirteen microsatellite loci. These 89 are some of the D. alata accessions conserved by the International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria. A total of 97 alleles were detected with an average allele number of 7.46 per locus. Polymorphism information content (PIC) mean value of 0.65 showed existence of variability among the accessions. Accessions from Nigeria showed highest gene diversity of 0.678 while those from Côte d’Ivoire had lowest diversity with 0.596. Observed mean heterozygosity value of 0.469 was observed. Cluster and principal coordinate analysis showed 8 major cluster groups. There was no relationship between relatedness of the accessions and their geographical area of collection. SSR markers proved to be effective to characterise studied D. alata germplasm. 2010-10 2018-01-16T12:03:36Z 2018-01-16T12:03:36Z Journal Article https://hdl.handle.net/10568/90348 en Limited Access Obidiegwu, J.E., Asiedu, R., Ene-Obong, E.E., Muoneke, C.O. & Kolesnikova-Allen, M. (2010). Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats. Journal of Food, Agriculture & Environment, 7(3&4), 634-638.
spellingShingle core germplasm
dioscorea alata
genetic variation
microsatellite
yams
electrophoresis
heterozygosity
polymorphic
Obidiegwu, J.E.
Asiedu, Robert
Ene-Obong, E.E.
Muoneke, C.O.
Kolesnikova-Allen, M.
Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats
title Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats
title_full Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats
title_fullStr Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats
title_full_unstemmed Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats
title_short Genetic characterization of some water yam (Dioscorea alata L.) in West Africa with simple sequence repeats
title_sort genetic characterization of some water yam dioscorea alata l in west africa with simple sequence repeats
topic core germplasm
dioscorea alata
genetic variation
microsatellite
yams
electrophoresis
heterozygosity
polymorphic
url https://hdl.handle.net/10568/90348
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