Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA
The traditional liquid nitrogen DNA extraction method is expensive and tedious. There is, therefore, the need for cheaper and faster methods of DNA extraction for efficient application of marker assisted selection (MAS) for breeding for striga resistance in cowpea to be able...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/90319 |
| _version_ | 1855520720928374784 |
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| author | Yusuf, M. Muranaka, S. Zaria, A.A. |
| author_browse | Muranaka, S. Yusuf, M. Zaria, A.A. |
| author_facet | Yusuf, M. Muranaka, S. Zaria, A.A. |
| author_sort | Yusuf, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The traditional liquid nitrogen DNA extraction method is expensive and tedious. There is, therefore, the need for cheaper and faster methods of DNA extraction for efficient application of marker assisted selection (MAS) for breeding for striga resistance in cowpea to be able to handle large number of samples at a time. Two DNA extraction methods; Hosaka (2004) and DJ Kim Whatmann paper (unpublished) were tested on cowpea line IT06K-5-83 under different conditions. The results revealed that both methods with modifications can work with cowpea. However, Hosaka (2004) method seems to be more promising than the other method for DNA extraction in cowpea because it gives better and more consistent DNA band. |
| format | Journal Article |
| id | CGSpace90319 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| record_format | dspace |
| spelling | CGSpace903192023-02-15T06:49:04Z Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA Yusuf, M. Muranaka, S. Zaria, A.A. striga resistance dna extraction methods marker assisted selection cowpea dna rapid isolation liquid nitrogen ethanol and vortexing The traditional liquid nitrogen DNA extraction method is expensive and tedious. There is, therefore, the need for cheaper and faster methods of DNA extraction for efficient application of marker assisted selection (MAS) for breeding for striga resistance in cowpea to be able to handle large number of samples at a time. Two DNA extraction methods; Hosaka (2004) and DJ Kim Whatmann paper (unpublished) were tested on cowpea line IT06K-5-83 under different conditions. The results revealed that both methods with modifications can work with cowpea. However, Hosaka (2004) method seems to be more promising than the other method for DNA extraction in cowpea because it gives better and more consistent DNA band. 2009 2018-01-16T12:03:30Z 2018-01-16T12:03:30Z Journal Article https://hdl.handle.net/10568/90319 en Open Access Yusuf, M., Muranaka, S. & Zaria, A.A. (2009). Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA. African Journal of Biotechnology, 8(23), 6518-6520. |
| spellingShingle | striga resistance dna extraction methods marker assisted selection cowpea dna rapid isolation liquid nitrogen ethanol and vortexing Yusuf, M. Muranaka, S. Zaria, A.A. Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA |
| title | Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA |
| title_full | Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA |
| title_fullStr | Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA |
| title_full_unstemmed | Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA |
| title_short | Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA |
| title_sort | application of hosaka and dj kim whatmann paper protocols for rapid isolation of cowpea dna |
| topic | striga resistance dna extraction methods marker assisted selection cowpea dna rapid isolation liquid nitrogen ethanol and vortexing |
| url | https://hdl.handle.net/10568/90319 |
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