Estimation of the nuclear DNA content in some representative of genus Dioscorea
Nuclear DNA content of 54 accessions of 6 Dioscorea species collected from West African countries were determined using flow cytometry. Observed ploidy levels are diploid (2x), triploid (3x), tetraploid (4x), hexaploid (6x) and octoploid (8x). DNA index varied from 0.36 to 1.54 depending on the cult...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/90219 |
| _version_ | 1855529013019148288 |
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| author | Obidiegwu, J.E. Rodríguez, E. Ene-Obong, E.E. Loureiro, J. Muoneke, C.O. Santos, C. Kolesnikova-Allen, M. Asiedu, Robert |
| author_browse | Asiedu, Robert Ene-Obong, E.E. Kolesnikova-Allen, M. Loureiro, J. Muoneke, C.O. Obidiegwu, J.E. Rodríguez, E. Santos, C. |
| author_facet | Obidiegwu, J.E. Rodríguez, E. Ene-Obong, E.E. Loureiro, J. Muoneke, C.O. Santos, C. Kolesnikova-Allen, M. Asiedu, Robert |
| author_sort | Obidiegwu, J.E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Nuclear DNA content of 54 accessions of 6 Dioscorea species collected from West African countries were determined using flow cytometry. Observed ploidy levels are diploid (2x), triploid (3x), tetraploid (4x), hexaploid (6x) and octoploid (8x). DNA index varied from 0.36 to 1.54 depending on the cultivar and considered specie. Estimated nuclear DNA sizes ranged from 0.702 ± 0.004 pg for G1 nuclei of diploid Dioscorea dumetorum to 2.573 ± 0.020 pg for G1 nuclei of octoploid Dioscorea cayenensis. Our result showed that there is a decrease in genome size with higher ploidy level. The study contributes a step towards a genome understanding of this group of plants |
| format | Journal Article |
| id | CGSpace90219 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| record_format | dspace |
| spelling | CGSpace902192023-06-12T15:52:19Z Estimation of the nuclear DNA content in some representative of genus Dioscorea Obidiegwu, J.E. Rodríguez, E. Ene-Obong, E.E. Loureiro, J. Muoneke, C.O. Santos, C. Kolesnikova-Allen, M. Asiedu, Robert dioscorea dna content flow cytometry genome ploidy level Nuclear DNA content of 54 accessions of 6 Dioscorea species collected from West African countries were determined using flow cytometry. Observed ploidy levels are diploid (2x), triploid (3x), tetraploid (4x), hexaploid (6x) and octoploid (8x). DNA index varied from 0.36 to 1.54 depending on the cultivar and considered specie. Estimated nuclear DNA sizes ranged from 0.702 ± 0.004 pg for G1 nuclei of diploid Dioscorea dumetorum to 2.573 ± 0.020 pg for G1 nuclei of octoploid Dioscorea cayenensis. Our result showed that there is a decrease in genome size with higher ploidy level. The study contributes a step towards a genome understanding of this group of plants 2009 2018-01-15T10:50:54Z 2018-01-15T10:50:54Z Journal Article https://hdl.handle.net/10568/90219 en Open Access Obidiegwu, J.E., Rodriguez, E., Ene-Obong, E.E., Loureiro, J., Muoneke, C.O., Santos, C., ... & Asiedu, R. (2009). Estimation of the nuclear DNA content in some representative of genus Dioscorea. Scientific Research and Essays, 4(5), 448-452. |
| spellingShingle | dioscorea dna content flow cytometry genome ploidy level Obidiegwu, J.E. Rodríguez, E. Ene-Obong, E.E. Loureiro, J. Muoneke, C.O. Santos, C. Kolesnikova-Allen, M. Asiedu, Robert Estimation of the nuclear DNA content in some representative of genus Dioscorea |
| title | Estimation of the nuclear DNA content in some representative of genus Dioscorea |
| title_full | Estimation of the nuclear DNA content in some representative of genus Dioscorea |
| title_fullStr | Estimation of the nuclear DNA content in some representative of genus Dioscorea |
| title_full_unstemmed | Estimation of the nuclear DNA content in some representative of genus Dioscorea |
| title_short | Estimation of the nuclear DNA content in some representative of genus Dioscorea |
| title_sort | estimation of the nuclear dna content in some representative of genus dioscorea |
| topic | dioscorea dna content flow cytometry genome ploidy level |
| url | https://hdl.handle.net/10568/90219 |
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