Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.)
Highly virulent, slow-growing grey (SGG); moderately virulent, fast-growing salmon (FGS); and avirulent/weakly virulent, fast-growing grey (FGG) forms of Colletotrichum gloeosporioides have been described from yam (Dioscorea spp.), but little is known about their chemodiversity or the role of toxins...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2009
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/90179 |
| _version_ | 1855530028261965824 |
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| author | Abang, Mathew M. Abraham, W.R. Asiedu, Robert Hoffmann, P. Wolf, G. Winter, S. |
| author_browse | Abang, Mathew M. Abraham, W.R. Asiedu, Robert Hoffmann, P. Winter, S. Wolf, G. |
| author_facet | Abang, Mathew M. Abraham, W.R. Asiedu, Robert Hoffmann, P. Wolf, G. Winter, S. |
| author_sort | Abang, Mathew M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Highly virulent, slow-growing grey (SGG); moderately virulent, fast-growing salmon (FGS); and avirulent/weakly virulent, fast-growing grey (FGG) forms of Colletotrichum gloeosporioides have been described from yam (Dioscorea spp.), but little is known about their chemodiversity or the role of toxins in their pathogenesis. Secondary metabolite profiles in high performance tlc (hptlc) showed that the pathogenic SGG and FGS forms have a chemotype (A or B) that is distinct from the non-pathogenic FGG form (chemotype C). Crude extracts of 35-d-old Czapek–Dox yeast broth cultures of FGS and SGG isolates caused tissue necrosis on treated yam leaves but not those of FGG isolates. Extracts from uninoculated broth cultures showed no phytotoxic activity. Toxicity of the culture filtrate was not host specific and toxic substances were thermostable. Dioscorea genotypes with varying levels of resistance to anthracnose differed in their sensitivity to crude toxin extract of FGS (Cg33) and SGG (Cg25) isolates, indicating that these extracts may be useful in evaluating host resistance to anthracnose in vitro. Analysis of two toxin fractions unique to the pathogenic FGS and SGG forms using hlpc, mass spectrometry and nuclear magnetic resonance suggested the presence of a low molecular weight amide peptide. However, possibly due to low yield and the presence of impurities, the chemical structure of the compound(s) could not be fully elucidated. |
| format | Journal Article |
| id | CGSpace90179 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace901792025-01-27T15:00:52Z Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.) Abang, Mathew M. Abraham, W.R. Asiedu, Robert Hoffmann, P. Wolf, G. Winter, S. anthracnose chemotype chromatography colletotrichum gloeosporioides dioscorea mass spectrometry metabolite profiling nmr phytotoxin Highly virulent, slow-growing grey (SGG); moderately virulent, fast-growing salmon (FGS); and avirulent/weakly virulent, fast-growing grey (FGG) forms of Colletotrichum gloeosporioides have been described from yam (Dioscorea spp.), but little is known about their chemodiversity or the role of toxins in their pathogenesis. Secondary metabolite profiles in high performance tlc (hptlc) showed that the pathogenic SGG and FGS forms have a chemotype (A or B) that is distinct from the non-pathogenic FGG form (chemotype C). Crude extracts of 35-d-old Czapek–Dox yeast broth cultures of FGS and SGG isolates caused tissue necrosis on treated yam leaves but not those of FGG isolates. Extracts from uninoculated broth cultures showed no phytotoxic activity. Toxicity of the culture filtrate was not host specific and toxic substances were thermostable. Dioscorea genotypes with varying levels of resistance to anthracnose differed in their sensitivity to crude toxin extract of FGS (Cg33) and SGG (Cg25) isolates, indicating that these extracts may be useful in evaluating host resistance to anthracnose in vitro. Analysis of two toxin fractions unique to the pathogenic FGS and SGG forms using hlpc, mass spectrometry and nuclear magnetic resonance suggested the presence of a low molecular weight amide peptide. However, possibly due to low yield and the presence of impurities, the chemical structure of the compound(s) could not be fully elucidated. 2009-01 2018-01-15T10:50:45Z 2018-01-15T10:50:45Z Journal Article https://hdl.handle.net/10568/90179 en Limited Access Elsevier Abang, M.M., Abraham, W.R., Asiedu, R., Hoffmann, P., Wolf, G. & Winter, S. (2009). Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.). Mycological Research, 113(1), 130-140. |
| spellingShingle | anthracnose chemotype chromatography colletotrichum gloeosporioides dioscorea mass spectrometry metabolite profiling nmr phytotoxin Abang, Mathew M. Abraham, W.R. Asiedu, Robert Hoffmann, P. Wolf, G. Winter, S. Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.) |
| title | Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.) |
| title_full | Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.) |
| title_fullStr | Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.) |
| title_full_unstemmed | Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.) |
| title_short | Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.) |
| title_sort | secondary metabolite profile and phytotoxic activity of genetically distinct forms of colletotrichum gloeosporioides from yam dioscorea spp |
| topic | anthracnose chemotype chromatography colletotrichum gloeosporioides dioscorea mass spectrometry metabolite profiling nmr phytotoxin |
| url | https://hdl.handle.net/10568/90179 |
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