Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines
Development of pro-vitamin A (PVA) biofortified staple crops has been promoted as an option for addressing vitamin A deficiency in humans. Recently, crtRBI, lcyE and zep1 genes of carotenoid biosynthesis pathway have been identified as contributors to PVA accumulation in maize endosperm. High phenot...
| Autores principales: | , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/89725 |
| _version_ | 1855542417095131136 |
|---|---|
| author | Gebremeskel, S. García Oliveira, A.L. Menkir, A. Adetimirin, Victor O. Gedil, Melaku A |
| author_browse | Adetimirin, Victor O. García Oliveira, A.L. Gebremeskel, S. Gedil, Melaku A Menkir, A. |
| author_facet | Gebremeskel, S. García Oliveira, A.L. Menkir, A. Adetimirin, Victor O. Gedil, Melaku A |
| author_sort | Gebremeskel, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Development of pro-vitamin A (PVA) biofortified staple crops has been promoted as an option for addressing vitamin A deficiency in humans. Recently, crtRBI, lcyE and zep1 genes of carotenoid biosynthesis pathway have been identified as contributors to PVA accumulation in maize endosperm. High phenotyping cost and narrow genetic base for these nutritionally important compounds have been regarded as the major challenges. We evaluated the fidelity of existing functional markers along with new markers for PVA carotenoids and assessed the diversity of 108 maize inbreds using SSR markers. Among the existing markers, crtRB1-5′TE and crtRB1-3′TE exhibited highly significant association with PVA whereas marker Zep-SNP(801), developed in this study, was associated only with α-carotene. Collectively, a substantial improvement in the PVA content, including β-carotene, was observed with increased number of total favourable alleles. Cluster analysis based on SSR produced two major groups, each with three and two sub-groups, respectively. The inbreds containing favourable alleles of the functional markers were present in all sub-groups. Finally, this genetically diverse inbreds with high PVA can be used in breeding programme using marker-assisted selection to enhance PVA content in maize hybrids and cultivars developed for lowlands in sub-Saharan Africa. |
| format | Journal Article |
| id | CGSpace89725 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace897252024-05-01T08:20:00Z Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines Gebremeskel, S. García Oliveira, A.L. Menkir, A. Adetimirin, Victor O. Gedil, Melaku A maize diversity carotenoids biosynthesis vitamin a Development of pro-vitamin A (PVA) biofortified staple crops has been promoted as an option for addressing vitamin A deficiency in humans. Recently, crtRBI, lcyE and zep1 genes of carotenoid biosynthesis pathway have been identified as contributors to PVA accumulation in maize endosperm. High phenotyping cost and narrow genetic base for these nutritionally important compounds have been regarded as the major challenges. We evaluated the fidelity of existing functional markers along with new markers for PVA carotenoids and assessed the diversity of 108 maize inbreds using SSR markers. Among the existing markers, crtRB1-5′TE and crtRB1-3′TE exhibited highly significant association with PVA whereas marker Zep-SNP(801), developed in this study, was associated only with α-carotene. Collectively, a substantial improvement in the PVA content, including β-carotene, was observed with increased number of total favourable alleles. Cluster analysis based on SSR produced two major groups, each with three and two sub-groups, respectively. The inbreds containing favourable alleles of the functional markers were present in all sub-groups. Finally, this genetically diverse inbreds with high PVA can be used in breeding programme using marker-assisted selection to enhance PVA content in maize hybrids and cultivars developed for lowlands in sub-Saharan Africa. 2018-01 2017-12-13T12:29:34Z 2017-12-13T12:29:34Z Journal Article https://hdl.handle.net/10568/89725 en Limited Access Elsevier Gebremeskel, S., Garcia-Oliveira, A.L., Menkir, A., Adetimirin, V. & Gedil, M. (2018). Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines. Journal of Cereal Science, 79, 27-34. |
| spellingShingle | maize diversity carotenoids biosynthesis vitamin a Gebremeskel, S. García Oliveira, A.L. Menkir, A. Adetimirin, Victor O. Gedil, Melaku A Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines |
| title | Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines |
| title_full | Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines |
| title_fullStr | Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines |
| title_full_unstemmed | Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines |
| title_short | Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines |
| title_sort | effectiveness of predictive markers for marker assisted selection of pro vitamin a carotenoids in medium late maturing maize zea mays l inbred lines |
| topic | maize diversity carotenoids biosynthesis vitamin a |
| url | https://hdl.handle.net/10568/89725 |
| work_keys_str_mv | AT gebremeskels effectivenessofpredictivemarkersformarkerassistedselectionofprovitaminacarotenoidsinmediumlatematuringmaizezeamayslinbredlines AT garciaoliveiraal effectivenessofpredictivemarkersformarkerassistedselectionofprovitaminacarotenoidsinmediumlatematuringmaizezeamayslinbredlines AT menkira effectivenessofpredictivemarkersformarkerassistedselectionofprovitaminacarotenoidsinmediumlatematuringmaizezeamayslinbredlines AT adetimirinvictoro effectivenessofpredictivemarkersformarkerassistedselectionofprovitaminacarotenoidsinmediumlatematuringmaizezeamayslinbredlines AT gedilmelakua effectivenessofpredictivemarkersformarkerassistedselectionofprovitaminacarotenoidsinmediumlatematuringmaizezeamayslinbredlines |