Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation
White lupin is one of the four economically important species of the Lupinus genus and is an important grain legume in the Ethiopian farming system. However, there has been limited research effort to characterize the Ethiopian white lupin landraces. Fifteen polymorphic simple sequence repeat (SSR) m...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/89713 |
| _version_ | 1855535994980270080 |
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| author | Tiruneh, Mulugeta Atnaf Yao, N. Kyallo, Martina M. Dagne, K. Wegary, D. Tesfaye, K. |
| author_browse | Dagne, K. Kyallo, Martina M. Tesfaye, K. Tiruneh, Mulugeta Atnaf Wegary, D. Yao, N. |
| author_facet | Tiruneh, Mulugeta Atnaf Yao, N. Kyallo, Martina M. Dagne, K. Wegary, D. Tesfaye, K. |
| author_sort | Tiruneh, Mulugeta Atnaf |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | White lupin is one of the four economically important species of the Lupinus genus and is an important grain legume in the Ethiopian farming system. However, there has been limited research effort to characterize the Ethiopian white lupin landraces. Fifteen polymorphic simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of 212 Ethiopian white lupin (Lupinus albus) landraces and two genotypes from different species (Lupinus angustifolius and Lupinus mutabilis) were used as out-group. The SSR markers revealed 108 different alleles, 98 of them from 212 landraces and 10 from out-group genotypes, with an average of 6.5 alleles per locus. The average gene diversity was 0.31. Twenty eight landraces harbored one or more private alleles from the total of 28 private alleles identified in the 212 white lupin accessions. Seventy-seven rare alleles with a frequency of less than 5% were identified and accounted for 78.6% of the total alleles detected. Analysis of molecular variance (AMOVA) showed that 92% of allelic diversity was attributed to individual accessions within populations while only 8% was distributed among populations. At 70% similarity level, the UPGMA dendrogram resulted in the formation of 13 clusters comprised of 2 to 136 landraces, with the out-group genotypes and five landraces remaining distinct and ungrouped. Population differentiation and genetic distance were relatively high between Gondar and Ethiopian white lupin populations collected by Australians. A model-based population structure analysis divided the white lupin landraces into two populations. All Ethiopian white lupin landrace populations, except most of the landraces collected by Australians (77%) and about 44% from Awi, were grouped together with significant admixtures. The study also suggested that 34 accessions, as core collections, were sufficient to retain 100% of SSR diversity. These accessions (core G-34) represent 16% of the whole 212 Ethiopian white lupin accessions and populations from West Gojam, Awi and Australian collections contributed more accessions to the core collection. |
| format | Journal Article |
| id | CGSpace89713 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace897132024-03-06T10:16:43Z Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation Tiruneh, Mulugeta Atnaf Yao, N. Kyallo, Martina M. Dagne, K. Wegary, D. Tesfaye, K. legumes biodiversity crops White lupin is one of the four economically important species of the Lupinus genus and is an important grain legume in the Ethiopian farming system. However, there has been limited research effort to characterize the Ethiopian white lupin landraces. Fifteen polymorphic simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of 212 Ethiopian white lupin (Lupinus albus) landraces and two genotypes from different species (Lupinus angustifolius and Lupinus mutabilis) were used as out-group. The SSR markers revealed 108 different alleles, 98 of them from 212 landraces and 10 from out-group genotypes, with an average of 6.5 alleles per locus. The average gene diversity was 0.31. Twenty eight landraces harbored one or more private alleles from the total of 28 private alleles identified in the 212 white lupin accessions. Seventy-seven rare alleles with a frequency of less than 5% were identified and accounted for 78.6% of the total alleles detected. Analysis of molecular variance (AMOVA) showed that 92% of allelic diversity was attributed to individual accessions within populations while only 8% was distributed among populations. At 70% similarity level, the UPGMA dendrogram resulted in the formation of 13 clusters comprised of 2 to 136 landraces, with the out-group genotypes and five landraces remaining distinct and ungrouped. Population differentiation and genetic distance were relatively high between Gondar and Ethiopian white lupin populations collected by Australians. A model-based population structure analysis divided the white lupin landraces into two populations. All Ethiopian white lupin landrace populations, except most of the landraces collected by Australians (77%) and about 44% from Awi, were grouped together with significant admixtures. The study also suggested that 34 accessions, as core collections, were sufficient to retain 100% of SSR diversity. These accessions (core G-34) represent 16% of the whole 212 Ethiopian white lupin accessions and populations from West Gojam, Awi and Australian collections contributed more accessions to the core collection. 2017-11-30 2017-12-13T08:46:53Z 2017-12-13T08:46:53Z Journal Article https://hdl.handle.net/10568/89713 en Open Access Public Library of Science Atnaf, M., Yao, N., Kyalo, M., Dagne, K., Wegary, D. and Tesfaye, K. 2017. Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation. PLoS ONE 12(11): e0188696. |
| spellingShingle | legumes biodiversity crops Tiruneh, Mulugeta Atnaf Yao, N. Kyallo, Martina M. Dagne, K. Wegary, D. Tesfaye, K. Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation |
| title | Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation |
| title_full | Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation |
| title_fullStr | Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation |
| title_full_unstemmed | Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation |
| title_short | Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation |
| title_sort | molecular genetic diversity and population structure of ethiopian white lupin landraces implications for breeding and conservation |
| topic | legumes biodiversity crops |
| url | https://hdl.handle.net/10568/89713 |
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