Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities
One of the most important crops cultivated around the world is coffee. There are two main cultivated species, Coffea arabica and C. canephora. Both species are difficult to improve through conventional breeding, taking at least 20 years to produce a new cultivar. Biotechnological tools such as genet...
| Main Authors: | , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2017
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/89214 |
| _version_ | 1855529444355080192 |
|---|---|
| author | Campos, N.A. Panis, Bartholomeus Carpentier, Sebastien C. |
| author_browse | Campos, N.A. Carpentier, Sebastien C. Panis, Bartholomeus |
| author_facet | Campos, N.A. Panis, Bartholomeus Carpentier, Sebastien C. |
| author_sort | Campos, N.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | One of the most important crops cultivated around the world is coffee. There are two main cultivated species, Coffea arabica and C. canephora. Both species are difficult to improve through conventional breeding, taking at least 20 years to produce a new cultivar. Biotechnological tools such as genetic transformation, micropropagation and somatic embryogenesis (SE) have been extensively studied in order to provide practical results for coffee improvement. While genetic transformation got many attention in the past and is booming with the CRISPR technology, micropropagation and SE are still the major bottle neck and urgently need more attention. The methodologies to induce SE and the further development of the embryos are genotype-dependent, what leads to an almost empirical development of specific protocols for each cultivar or clone. This is a serious limitation and excludes a general comprehensive understanding of the process as a whole. The aim of this review is to provide an overview of which achievements and molecular insights have been gained in (coffee) somatic embryogenesis and encourage researchers to invest further in the in vitro technology and combine it with the latest omics techniques (genomics, transcriptomics, proteomics, metabolomics, and phenomics). We conclude that the evolution of biotechnology and the integration of omics technologies offer great opportunities to (i) optimize the production process of SE and the subsequent conversion into rooted plantlets and (ii) to screen for possible somaclonal variation. However, currently the usage of the latest biotechnology did not pass the stage beyond proof of potential and needs to further improve. |
| format | Journal Article |
| id | CGSpace89214 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace892142025-11-12T05:43:49Z Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities Campos, N.A. Panis, Bartholomeus Carpentier, Sebastien C. coffea in vitro culture somatic embryogenesis biotechnology somaclonal variation One of the most important crops cultivated around the world is coffee. There are two main cultivated species, Coffea arabica and C. canephora. Both species are difficult to improve through conventional breeding, taking at least 20 years to produce a new cultivar. Biotechnological tools such as genetic transformation, micropropagation and somatic embryogenesis (SE) have been extensively studied in order to provide practical results for coffee improvement. While genetic transformation got many attention in the past and is booming with the CRISPR technology, micropropagation and SE are still the major bottle neck and urgently need more attention. The methodologies to induce SE and the further development of the embryos are genotype-dependent, what leads to an almost empirical development of specific protocols for each cultivar or clone. This is a serious limitation and excludes a general comprehensive understanding of the process as a whole. The aim of this review is to provide an overview of which achievements and molecular insights have been gained in (coffee) somatic embryogenesis and encourage researchers to invest further in the in vitro technology and combine it with the latest omics techniques (genomics, transcriptomics, proteomics, metabolomics, and phenomics). We conclude that the evolution of biotechnology and the integration of omics technologies offer great opportunities to (i) optimize the production process of SE and the subsequent conversion into rooted plantlets and (ii) to screen for possible somaclonal variation. However, currently the usage of the latest biotechnology did not pass the stage beyond proof of potential and needs to further improve. 2017 2017-11-07T16:26:28Z 2017-11-07T16:26:28Z Journal Article https://hdl.handle.net/10568/89214 en Open Access application/pdf Frontiers Media Campos, N. A.; Panis, B.; Carpentier, S.C. (2017) Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities. Frontiers in Plant Science 8:1460. ISSN: 1664-462X |
| spellingShingle | coffea in vitro culture somatic embryogenesis biotechnology somaclonal variation Campos, N.A. Panis, Bartholomeus Carpentier, Sebastien C. Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities |
| title | Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities |
| title_full | Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities |
| title_fullStr | Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities |
| title_full_unstemmed | Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities |
| title_short | Somatic embryogenesis in coffee: the evolution of biotechnology and the integration of omics technologies offer great opportunities |
| title_sort | somatic embryogenesis in coffee the evolution of biotechnology and the integration of omics technologies offer great opportunities |
| topic | coffea in vitro culture somatic embryogenesis biotechnology somaclonal variation |
| url | https://hdl.handle.net/10568/89214 |
| work_keys_str_mv | AT camposna somaticembryogenesisincoffeetheevolutionofbiotechnologyandtheintegrationofomicstechnologiesoffergreatopportunities AT panisbartholomeus somaticembryogenesisincoffeetheevolutionofbiotechnologyandtheintegrationofomicstechnologiesoffergreatopportunities AT carpentiersebastienc somaticembryogenesisincoffeetheevolutionofbiotechnologyandtheintegrationofomicstechnologiesoffergreatopportunities |