Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis
The vitellogenin receptor (VgR), which belongs to the low-density lipoprotein receptors(LDLR) family, regulates the absorption of yolk protein accumulated in developing oocytes during oogenesis. In the present study, the full sequence of Rhipicephalus microplus VgR (RmVgR) and the partial sequence o...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/89178 |
| _version_ | 1855530168904318976 |
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| author | Seixas, A. Alzugaray, M.F. Tirloni, T. Parizi, L.F. Pinto, A.F.M. Githaka, Naftaly W. Konnai, S. Ohashi, K. Yates, J.R. Termignoni, C. Silva Vaz, I. da |
| author_browse | Alzugaray, M.F. Githaka, Naftaly W. Konnai, S. Ohashi, K. Parizi, L.F. Pinto, A.F.M. Seixas, A. Silva Vaz, I. da Termignoni, C. Tirloni, T. Yates, J.R. |
| author_facet | Seixas, A. Alzugaray, M.F. Tirloni, T. Parizi, L.F. Pinto, A.F.M. Githaka, Naftaly W. Konnai, S. Ohashi, K. Yates, J.R. Termignoni, C. Silva Vaz, I. da |
| author_sort | Seixas, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The vitellogenin receptor (VgR), which belongs to the low-density lipoprotein receptors(LDLR) family, regulates the absorption of yolk protein accumulated in developing oocytes during oogenesis. In the present study, the full sequence of Rhipicephalus microplus VgR (RmVgR) and the partial sequence of Rhipicephalus appendiculatus VgR (RaVgR) ORF were determined and cloned. The RmVgR amino acid sequence contains the five highly conserved structural motifs characteristic of LDLR superfamily members, the same overall structure as observed in other species. Phylogenetic analysis separated VgRs in two major groups, corresponding to receptors from acarines and insects. Consistent with observations from other arthropods, RmVgR was specifically expressed in the ovarian tissue and its peak of expression occurs in females that are detaching from the host. Silencing with RmVgR dsRNA reduced VgR expression, which resulted in reduced fertility, evidenced by a decrease in the number of larvae. The present study confirms RmVgR is a specific receptor involved in yolk protein uptake and oocyte maturation in R. microplus, playing an important role in tick reproduction. |
| format | Journal Article |
| id | CGSpace89178 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace891782024-01-12T10:00:54Z Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis Seixas, A. Alzugaray, M.F. Tirloni, T. Parizi, L.F. Pinto, A.F.M. Githaka, Naftaly W. Konnai, S. Ohashi, K. Yates, J.R. Termignoni, C. Silva Vaz, I. da oogenesis ova rhipicephalus vitellogenins The vitellogenin receptor (VgR), which belongs to the low-density lipoprotein receptors(LDLR) family, regulates the absorption of yolk protein accumulated in developing oocytes during oogenesis. In the present study, the full sequence of Rhipicephalus microplus VgR (RmVgR) and the partial sequence of Rhipicephalus appendiculatus VgR (RaVgR) ORF were determined and cloned. The RmVgR amino acid sequence contains the five highly conserved structural motifs characteristic of LDLR superfamily members, the same overall structure as observed in other species. Phylogenetic analysis separated VgRs in two major groups, corresponding to receptors from acarines and insects. Consistent with observations from other arthropods, RmVgR was specifically expressed in the ovarian tissue and its peak of expression occurs in females that are detaching from the host. Silencing with RmVgR dsRNA reduced VgR expression, which resulted in reduced fertility, evidenced by a decrease in the number of larvae. The present study confirms RmVgR is a specific receptor involved in yolk protein uptake and oocyte maturation in R. microplus, playing an important role in tick reproduction. 2018-01 2017-11-01T12:23:00Z 2017-11-01T12:23:00Z Journal Article https://hdl.handle.net/10568/89178 en Limited Access Elsevier Seixas, A., Alzugaray, M.F., Tirloni, T., Parizi, L.F., Pinto, A.F.M., Githaka, N.W., Konnai, S., Ohashi, K., Yates, J.R., III, Termignoni, C. and Silva Vaz Jr., I. da. 2018. Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis. Ticks and Tick-borne Diseases 9(1): 72–81. |
| spellingShingle | oogenesis ova rhipicephalus vitellogenins Seixas, A. Alzugaray, M.F. Tirloni, T. Parizi, L.F. Pinto, A.F.M. Githaka, Naftaly W. Konnai, S. Ohashi, K. Yates, J.R. Termignoni, C. Silva Vaz, I. da Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis |
| title | Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis |
| title_full | Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis |
| title_fullStr | Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis |
| title_full_unstemmed | Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis |
| title_short | Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis |
| title_sort | expression profile of rhipicephalus microplus vitellogenin receptor during oogenesis |
| topic | oogenesis ova rhipicephalus vitellogenins |
| url | https://hdl.handle.net/10568/89178 |
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