Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum
Vitamin A deficiency (VAD) is prevalent in most developing countries. Increasing provitamin A carotenoid content in staple crops such as yam, maize and cassava can minimize this deficiency. Yam (Dioscorea species) is a major staple food in many tropical and subtropical countries. Despite its wide co...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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2010
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| Online Access: | https://hdl.handle.net/10568/89077 |
| _version_ | 1855537910753787904 |
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| author | Ferede, R. Maziya-Dixon, B.B. Alamu, O.E. Asiedu, Robert |
| author_browse | Alamu, O.E. Asiedu, Robert Ferede, R. Maziya-Dixon, B.B. |
| author_facet | Ferede, R. Maziya-Dixon, B.B. Alamu, O.E. Asiedu, Robert |
| author_sort | Ferede, R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Vitamin A deficiency (VAD) is prevalent in most developing countries. Increasing provitamin A carotenoid content in staple crops such as yam, maize and cassava can minimize this deficiency. Yam (Dioscorea species) is a major staple food in many tropical and subtropical countries. Despite its wide consumption, there is limited information on the type and amount of carotenoids in different species. This study was conducted to update methods for extraction of carotenoids, to identify and quantify the major carotenoids and determine the provitamin A (PVA) content in Dioscorea dumetorum (trifoliate yam). The carotenoid components of Dioscorea dumetorum were extracted using solvent extraction, and major components were separated by high-performance liquid chromatography (HPLC) and identified by their UV/visible absorption spectral data and co-elution with reference standards using HPLC, Thin Layer Chromatography (TLC) and Open Column Chromatography (OCC). Results obtained indicate that the major carotenoids in the deep yellow fleshed trifoliate yam are trans-β-carotene-5,8-epoxide 9.4±3.9 μg g-1 (55.1% ), β-cryptoxanthin-5,6-epoxide 4.7±1.2 μg g-1 (27.0%), β-carotene-5,6-epoxide 1.7±0.07 μg g-1 (9.6%) and cis and trans β-carotene 1.2±0.4 μg g-1 (7.3%). Provitamin A content was calculated and the average provitamin A carotenoid in the deep yellow-fleshed genotypes was 8.9±3.2 μg g-1 representing 52% of the total carotenoid content. The profile of carotenoids and the high concentration of trans-β-carotene-5,8-epoxide found in the deep yellow-fleshed D. dumetorum are unique compared to other species of yam or other tuber roots such as cassava and sweet potato. |
| format | Journal Article |
| id | CGSpace89077 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| record_format | dspace |
| spelling | CGSpace890772023-02-15T07:12:48Z Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum Ferede, R. Maziya-Dixon, B.B. Alamu, O.E. Asiedu, Robert carotinoids yams dioscorea dumetorum vitamin a provitamin a carotene Vitamin A deficiency (VAD) is prevalent in most developing countries. Increasing provitamin A carotenoid content in staple crops such as yam, maize and cassava can minimize this deficiency. Yam (Dioscorea species) is a major staple food in many tropical and subtropical countries. Despite its wide consumption, there is limited information on the type and amount of carotenoids in different species. This study was conducted to update methods for extraction of carotenoids, to identify and quantify the major carotenoids and determine the provitamin A (PVA) content in Dioscorea dumetorum (trifoliate yam). The carotenoid components of Dioscorea dumetorum were extracted using solvent extraction, and major components were separated by high-performance liquid chromatography (HPLC) and identified by their UV/visible absorption spectral data and co-elution with reference standards using HPLC, Thin Layer Chromatography (TLC) and Open Column Chromatography (OCC). Results obtained indicate that the major carotenoids in the deep yellow fleshed trifoliate yam are trans-β-carotene-5,8-epoxide 9.4±3.9 μg g-1 (55.1% ), β-cryptoxanthin-5,6-epoxide 4.7±1.2 μg g-1 (27.0%), β-carotene-5,6-epoxide 1.7±0.07 μg g-1 (9.6%) and cis and trans β-carotene 1.2±0.4 μg g-1 (7.3%). Provitamin A content was calculated and the average provitamin A carotenoid in the deep yellow-fleshed genotypes was 8.9±3.2 μg g-1 representing 52% of the total carotenoid content. The profile of carotenoids and the high concentration of trans-β-carotene-5,8-epoxide found in the deep yellow-fleshed D. dumetorum are unique compared to other species of yam or other tuber roots such as cassava and sweet potato. 2010 2017-10-20T09:57:18Z 2017-10-20T09:57:18Z Journal Article https://hdl.handle.net/10568/89077 en Limited Access Ferede, R., Maziya-Dixon, B., Alamu, O.E. & Asiedu, R. (2010). Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum). Journal of Food, Agriculture & Environment, 8(3&4), 160-166. |
| spellingShingle | carotinoids yams dioscorea dumetorum vitamin a provitamin a carotene Ferede, R. Maziya-Dixon, B.B. Alamu, O.E. Asiedu, Robert Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum |
| title | Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum |
| title_full | Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum |
| title_fullStr | Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum |
| title_full_unstemmed | Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum |
| title_short | Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum |
| title_sort | identification and quantification of major carotenoids of deep yellow fleshed yam tropical dioscorea dumetorum |
| topic | carotinoids yams dioscorea dumetorum vitamin a provitamin a carotene |
| url | https://hdl.handle.net/10568/89077 |
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