Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease
Banana Xanthomonas wilt (BXW) disease threatens banana production and food security throughout East Africa. Natural resistance is lacking among common cultivars. Genetically modified (GM) bananas resistant to BXW disease were developed by inserting the hypersensitive response-assisting protein (Hrap...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2017
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/89019 |
| _version_ | 1855518857412739072 |
|---|---|
| author | Jin, Y. Goodman, R.E. Tetteh, A.O. Lu, M. Tripathi, L. |
| author_browse | Goodman, R.E. Jin, Y. Lu, M. Tetteh, A.O. Tripathi, L. |
| author_facet | Jin, Y. Goodman, R.E. Tetteh, A.O. Lu, M. Tripathi, L. |
| author_sort | Jin, Y. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Banana Xanthomonas wilt (BXW) disease threatens banana production and food security throughout East Africa. Natural resistance is lacking among common cultivars. Genetically modified (GM) bananas resistant to BXW disease were developed by inserting the hypersensitive response-assisting protein (Hrap) or/and the plant ferredoxin-like protein (Pflp) gene(s) from sweet pepper (Capsicum annuum). Several of these GM banana events showed 100% resistance to BXW disease under field conditions in Uganda. The current study evaluated the potential allergenicity and toxicity of the expressed proteins HRAP and PFLP based on evaluation of published information on the history of safe use of the natural source of the proteins as well as established bioinformatics sequence comparison methods to known allergens (www.AllergenOnline.org and NCBI Protein) and toxins (NCBI Protein). The results did not identify potential risks of allergy and toxicity to either HRAP or PFLP proteins expressed in the GM bananas that might suggest potential health risks to humans. We recognize that additional tests including stability of these proteins in pepsin assay, nutrient analysis and possibly an acute rodent toxicity assay may be required by national regulatory authorities. |
| format | Journal Article |
| id | CGSpace89019 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace890192025-11-11T10:08:14Z Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease Jin, Y. Goodman, R.E. Tetteh, A.O. Lu, M. Tripathi, L. bananas allergen toxin risk assessment hrap pflp genetically modified banana banana xanthomonas wilt bioinformatics toxicology food science Banana Xanthomonas wilt (BXW) disease threatens banana production and food security throughout East Africa. Natural resistance is lacking among common cultivars. Genetically modified (GM) bananas resistant to BXW disease were developed by inserting the hypersensitive response-assisting protein (Hrap) or/and the plant ferredoxin-like protein (Pflp) gene(s) from sweet pepper (Capsicum annuum). Several of these GM banana events showed 100% resistance to BXW disease under field conditions in Uganda. The current study evaluated the potential allergenicity and toxicity of the expressed proteins HRAP and PFLP based on evaluation of published information on the history of safe use of the natural source of the proteins as well as established bioinformatics sequence comparison methods to known allergens (www.AllergenOnline.org and NCBI Protein) and toxins (NCBI Protein). The results did not identify potential risks of allergy and toxicity to either HRAP or PFLP proteins expressed in the GM bananas that might suggest potential health risks to humans. We recognize that additional tests including stability of these proteins in pepsin assay, nutrient analysis and possibly an acute rodent toxicity assay may be required by national regulatory authorities. 2017-11 2017-10-16T08:13:26Z 2017-10-16T08:13:26Z Journal Article https://hdl.handle.net/10568/89019 en Open Access application/pdf Elsevier Jin, Y., Goodman, R.E., Tetteh, A.O., Lu, M. & Tripathi, L. (2017). Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease. Food and Chemical Toxicology, 109(1), 81-89. |
| spellingShingle | bananas allergen toxin risk assessment hrap pflp genetically modified banana banana xanthomonas wilt bioinformatics toxicology food science Jin, Y. Goodman, R.E. Tetteh, A.O. Lu, M. Tripathi, L. Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease |
| title | Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease |
| title_full | Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease |
| title_fullStr | Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease |
| title_full_unstemmed | Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease |
| title_short | Bioinformatics analysis to assess potential risks of allergenicity and toxicity of HRAP and PFLP proteins in genetically modified bananas resistant to Xanthomonas wilt disease |
| title_sort | bioinformatics analysis to assess potential risks of allergenicity and toxicity of hrap and pflp proteins in genetically modified bananas resistant to xanthomonas wilt disease |
| topic | bananas allergen toxin risk assessment hrap pflp genetically modified banana banana xanthomonas wilt bioinformatics toxicology food science |
| url | https://hdl.handle.net/10568/89019 |
| work_keys_str_mv | AT jiny bioinformaticsanalysistoassesspotentialrisksofallergenicityandtoxicityofhrapandpflpproteinsingeneticallymodifiedbananasresistanttoxanthomonaswiltdisease AT goodmanre bioinformaticsanalysistoassesspotentialrisksofallergenicityandtoxicityofhrapandpflpproteinsingeneticallymodifiedbananasresistanttoxanthomonaswiltdisease AT tettehao bioinformaticsanalysistoassesspotentialrisksofallergenicityandtoxicityofhrapandpflpproteinsingeneticallymodifiedbananasresistanttoxanthomonaswiltdisease AT lum bioinformaticsanalysistoassesspotentialrisksofallergenicityandtoxicityofhrapandpflpproteinsingeneticallymodifiedbananasresistanttoxanthomonaswiltdisease AT tripathil bioinformaticsanalysistoassesspotentialrisksofallergenicityandtoxicityofhrapandpflpproteinsingeneticallymodifiedbananasresistanttoxanthomonaswiltdisease |