Evidence for a latent viruslike agent in cassava
Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nuclear...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1987
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| Acceso en línea: | https://hdl.handle.net/10568/88328 |
| _version_ | 1855532564319567872 |
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| author | Gabriel, C.J. Walsh, R. Nolt, Barry L. |
| author_browse | Gabriel, C.J. Nolt, Barry L. Walsh, R. |
| author_facet | Gabriel, C.J. Walsh, R. Nolt, Barry L. |
| author_sort | Gabriel, C.J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nuclear-rich fraction isolated from dsRNA-containing Secundina plants. Viruslike particles were not detected in plants containing the L-dsRNA using a var. of purification procedures. L-dsRNA was also found in 2 non- Secundina cassava introductions. Smaller dsRNAs(S-dsRNAs), ranging mainly between 0.61-0.41 x 106 Mr, were also detected in some Secundina plants containing the L-dsRNA. The S-dsRNAs were not transmissible by mechanical inoculation or grafting. All dsRNAs could be eliminated by meristem-tip culture of infected plants. DsRNA analysis is recommended for indexing cassava for this latent viruslike agent. (AS) |
| format | Journal Article |
| id | CGSpace88328 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1987 |
| publishDateRange | 1987 |
| publishDateSort | 1987 |
| record_format | dspace |
| spelling | CGSpace883282024-01-17T12:58:34Z Evidence for a latent viruslike agent in cassava Gabriel, C.J. Walsh, R. Nolt, Barry L. manihot esculenta clones disease transmission analysis tissue culture cytology cytogenetics usa north america starch crops transmision de enfermedades análisis cultivo de tejidos citologia citogenetica ee.uu Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nuclear-rich fraction isolated from dsRNA-containing Secundina plants. Viruslike particles were not detected in plants containing the L-dsRNA using a var. of purification procedures. L-dsRNA was also found in 2 non- Secundina cassava introductions. Smaller dsRNAs(S-dsRNAs), ranging mainly between 0.61-0.41 x 106 Mr, were also detected in some Secundina plants containing the L-dsRNA. The S-dsRNAs were not transmissible by mechanical inoculation or grafting. All dsRNAs could be eliminated by meristem-tip culture of infected plants. DsRNA analysis is recommended for indexing cassava for this latent viruslike agent. (AS) 1987 2017-10-12T08:02:17Z 2017-10-12T08:02:17Z Journal Article https://hdl.handle.net/10568/88328 en Limited Access GABRIEL, C.J.; WALSH, R.; NOLT, B.L. 1987. Evidence for a latent viruslike agent in cassava. Phytopathology. 77(1):92-95. |
| spellingShingle | manihot esculenta clones disease transmission analysis tissue culture cytology cytogenetics usa north america starch crops transmision de enfermedades análisis cultivo de tejidos citologia citogenetica ee.uu Gabriel, C.J. Walsh, R. Nolt, Barry L. Evidence for a latent viruslike agent in cassava |
| title | Evidence for a latent viruslike agent in cassava |
| title_full | Evidence for a latent viruslike agent in cassava |
| title_fullStr | Evidence for a latent viruslike agent in cassava |
| title_full_unstemmed | Evidence for a latent viruslike agent in cassava |
| title_short | Evidence for a latent viruslike agent in cassava |
| title_sort | evidence for a latent viruslike agent in cassava |
| topic | manihot esculenta clones disease transmission analysis tissue culture cytology cytogenetics usa north america starch crops transmision de enfermedades análisis cultivo de tejidos citologia citogenetica ee.uu |
| url | https://hdl.handle.net/10568/88328 |
| work_keys_str_mv | AT gabrielcj evidenceforalatentviruslikeagentincassava AT walshr evidenceforalatentviruslikeagentincassava AT noltbarryl evidenceforalatentviruslikeagentincassava |