In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches
We report here the molecular and phenotypic features of a library of 31 562 insertion lines generated in the model japonica cultivar Nipponbare of rice (Oryza sativa L.), called Oryza Tag Line (OTL). Sixteen thousand eight hundred and fourteen T‐DNA and 12 410 Tos17 discrete insertion sites have bee...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/88270 |
| _version_ | 1855520682188734464 |
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| author | Lorieux, Mathias Blein, Mélisande Lozano, Jaime Bouniol, Matthieu Droc, Gaëtan Diévart, Anne Périn, Christophe Mieulet, Delphine Lanau, Nadege Bès, Martine Rouvière, Claire Gay, Céline Piffanelli, Pietro Larmande, Pierre Michel, Corinne Barnola, Isabelle Biderre-Petit, Corinne Sallaud, Christophe Pérez, Pascual Bourgis, Fabienne Ghesquière, Alain Gantet, Pascual Tohme, Joseph M. Morel, Jean-Benoit Guiderdoni, Emmanuel |
| author_browse | Barnola, Isabelle Biderre-Petit, Corinne Blein, Mélisande Bouniol, Matthieu Bourgis, Fabienne Bès, Martine Diévart, Anne Droc, Gaëtan Gantet, Pascual Gay, Céline Ghesquière, Alain Guiderdoni, Emmanuel Lanau, Nadege Larmande, Pierre Lorieux, Mathias Lozano, Jaime Michel, Corinne Mieulet, Delphine Morel, Jean-Benoit Piffanelli, Pietro Pérez, Pascual Périn, Christophe Rouvière, Claire Sallaud, Christophe Tohme, Joseph M. |
| author_facet | Lorieux, Mathias Blein, Mélisande Lozano, Jaime Bouniol, Matthieu Droc, Gaëtan Diévart, Anne Périn, Christophe Mieulet, Delphine Lanau, Nadege Bès, Martine Rouvière, Claire Gay, Céline Piffanelli, Pietro Larmande, Pierre Michel, Corinne Barnola, Isabelle Biderre-Petit, Corinne Sallaud, Christophe Pérez, Pascual Bourgis, Fabienne Ghesquière, Alain Gantet, Pascual Tohme, Joseph M. Morel, Jean-Benoit Guiderdoni, Emmanuel |
| author_sort | Lorieux, Mathias |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We report here the molecular and phenotypic features of a library of 31 562 insertion lines generated in the model japonica cultivar Nipponbare of rice (Oryza sativa L.), called Oryza Tag Line (OTL). Sixteen thousand eight hundred and fourteen T‐DNA and 12 410 Tos17 discrete insertion sites have been characterized in these lines. We estimate that 8686 predicted gene intervals—i.e. one‐fourth to one‐fifth of the estimated rice nontransposable element gene complement—are interrupted by sequence‐indexed T‐DNA (6563 genes) and/or Tos17 (2755 genes) inserts. Six hundred and forty‐three genes are interrupted by both T‐DNA and Tos17 inserts. High quality of the sequence indexation of the T2 seed samples was ascertained by several approaches. Field evaluation under agronomic conditions of 27 832 OTL has revealed that 18.2% exhibit at least one morphophysiological alteration in the T1 progeny plants. Screening 10 000 lines for altered response to inoculation by the fungal pathogen Magnaporthe oryzae allowed to observe 71 lines (0.7%) developing spontaneous lesions simulating disease mutants and 43 lines (0.4%) exhibiting an enhanced disease resistance or susceptibility. We show here that at least 3.5% (four of 114) of these alterations are tagged by the mutagens. The presence of allelic series of sequence‐indexed mutations in a gene among OTL that exhibit a convergent phenotype clearly increases the chance of establishing a linkage between alterations and inserts. This convergence approach is illustrated by the identification of the rice ortholog of AtPHO2, the disruption of which causes a lesion‐mimic phenotype owing to an over‐accumulation of phosphate, in nine lines bearing allelic insertions. |
| format | Journal Article |
| id | CGSpace88270 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace882702024-08-27T10:35:25Z In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches Lorieux, Mathias Blein, Mélisande Lozano, Jaime Bouniol, Matthieu Droc, Gaëtan Diévart, Anne Périn, Christophe Mieulet, Delphine Lanau, Nadege Bès, Martine Rouvière, Claire Gay, Céline Piffanelli, Pietro Larmande, Pierre Michel, Corinne Barnola, Isabelle Biderre-Petit, Corinne Sallaud, Christophe Pérez, Pascual Bourgis, Fabienne Ghesquière, Alain Gantet, Pascual Tohme, Joseph M. Morel, Jean-Benoit Guiderdoni, Emmanuel oryza sativa rice mutagenesis molecular genetics phenotypes dna arroz mutagénesis genética molecular fenotipos adn We report here the molecular and phenotypic features of a library of 31 562 insertion lines generated in the model japonica cultivar Nipponbare of rice (Oryza sativa L.), called Oryza Tag Line (OTL). Sixteen thousand eight hundred and fourteen T‐DNA and 12 410 Tos17 discrete insertion sites have been characterized in these lines. We estimate that 8686 predicted gene intervals—i.e. one‐fourth to one‐fifth of the estimated rice nontransposable element gene complement—are interrupted by sequence‐indexed T‐DNA (6563 genes) and/or Tos17 (2755 genes) inserts. Six hundred and forty‐three genes are interrupted by both T‐DNA and Tos17 inserts. High quality of the sequence indexation of the T2 seed samples was ascertained by several approaches. Field evaluation under agronomic conditions of 27 832 OTL has revealed that 18.2% exhibit at least one morphophysiological alteration in the T1 progeny plants. Screening 10 000 lines for altered response to inoculation by the fungal pathogen Magnaporthe oryzae allowed to observe 71 lines (0.7%) developing spontaneous lesions simulating disease mutants and 43 lines (0.4%) exhibiting an enhanced disease resistance or susceptibility. We show here that at least 3.5% (four of 114) of these alterations are tagged by the mutagens. The presence of allelic series of sequence‐indexed mutations in a gene among OTL that exhibit a convergent phenotype clearly increases the chance of establishing a linkage between alterations and inserts. This convergence approach is illustrated by the identification of the rice ortholog of AtPHO2, the disruption of which causes a lesion‐mimic phenotype owing to an over‐accumulation of phosphate, in nine lines bearing allelic insertions. 2012-06 2017-10-12T08:02:05Z 2017-10-12T08:02:05Z Journal Article https://hdl.handle.net/10568/88270 en Limited Access Wiley Lorieux, Mathias; Blein, Mélisande; Lozano, Jaime; Bouniol, Matthieu; Droc, Gaëtan; Diévart, Anne; Périn, Christophe; Mieulet, Delphine; Lanau, Nadege; Bès, Martine; Rouvière, Claire; Gay, Céline; Piffanelli, Pietro; Larmande, Pierre; Michel, Corinne; Barnola, Isabelle; Biderre-Petit, Corinne; Sallaud, Christophe; Perez, Pascual; Bourgis, Fabienne; Ghesquière, Alain; Gantet, Pascual; Tohme, Joseph M.; Morel, Jean Benoit; Guiderdoni, Emmanuel. 2012. In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches. Plant Biotechnology Journal. 10(5) 555-568. |
| spellingShingle | oryza sativa rice mutagenesis molecular genetics phenotypes dna arroz mutagénesis genética molecular fenotipos adn Lorieux, Mathias Blein, Mélisande Lozano, Jaime Bouniol, Matthieu Droc, Gaëtan Diévart, Anne Périn, Christophe Mieulet, Delphine Lanau, Nadege Bès, Martine Rouvière, Claire Gay, Céline Piffanelli, Pietro Larmande, Pierre Michel, Corinne Barnola, Isabelle Biderre-Petit, Corinne Sallaud, Christophe Pérez, Pascual Bourgis, Fabienne Ghesquière, Alain Gantet, Pascual Tohme, Joseph M. Morel, Jean-Benoit Guiderdoni, Emmanuel In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches |
| title | In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches |
| title_full | In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches |
| title_fullStr | In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches |
| title_full_unstemmed | In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches |
| title_short | In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches |
| title_sort | in depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches |
| topic | oryza sativa rice mutagenesis molecular genetics phenotypes dna arroz mutagénesis genética molecular fenotipos adn |
| url | https://hdl.handle.net/10568/88270 |
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