Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean
Simple sequence repeat (SSR) markers were used to classify 116 isolates of Phakopsora pachyrhizi, the cause of soyabean rust, collected from infected soyabean leaves in four agroecological zones in Nigeria. A high degree of genetic variation was observed within the sampled populations of P. pachyrhi...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2011
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/88162 |
| _version_ | 1855531181292912640 |
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| author | Twizeyimana, M. Ojiambo, P.S. Haudenshield, J.S. Caetano‐Anollés, G. Pedley, K.F. Bandyopadhyay, Ranajit Hartman, G.L. |
| author_browse | Bandyopadhyay, Ranajit Caetano‐Anollés, G. Hartman, G.L. Haudenshield, J.S. Ojiambo, P.S. Pedley, K.F. Twizeyimana, M. |
| author_facet | Twizeyimana, M. Ojiambo, P.S. Haudenshield, J.S. Caetano‐Anollés, G. Pedley, K.F. Bandyopadhyay, Ranajit Hartman, G.L. |
| author_sort | Twizeyimana, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Simple sequence repeat (SSR) markers were used to classify 116 isolates of Phakopsora pachyrhizi, the cause of soyabean rust, collected from infected soyabean leaves in four agroecological zones in Nigeria. A high degree of genetic variation was observed within the sampled populations of P. pachyrhizi. Eighty-four distinct genotypes were identified among three of the four agroecological zones. Nei’s average genetic diversity across geographical regions was 0·22. Hierarchical analysis of molecular variance showed low genetic differentiation among all populations of P. pachyrhizi. The majority (> 90%) of the genetic diversity was distributed within each soyabean field, while approximately 6% of the genetic diversity was distributed among fields within geographic regions. Low population differentiation was indicated by the low FST values among populations, suggesting a wide dispersal of identical genotypes on a regional scale. Phylogenetic analysis indicated a strictly clonal structure of the populations and five main groups were observed, with group II accounting for 30% of the entire population. Because of the asexual reproduction of P. pachyrhizi, single-step mutations in SSR genotypes are likely to account for the genetic differences within each group. |
| format | Journal Article |
| id | CGSpace88162 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace881622023-07-03T15:56:01Z Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean Twizeyimana, M. Ojiambo, P.S. Haudenshield, J.S. Caetano‐Anollés, G. Pedley, K.F. Bandyopadhyay, Ranajit Hartman, G.L. glycine max soybeans virulence phenotypes Simple sequence repeat (SSR) markers were used to classify 116 isolates of Phakopsora pachyrhizi, the cause of soyabean rust, collected from infected soyabean leaves in four agroecological zones in Nigeria. A high degree of genetic variation was observed within the sampled populations of P. pachyrhizi. Eighty-four distinct genotypes were identified among three of the four agroecological zones. Nei’s average genetic diversity across geographical regions was 0·22. Hierarchical analysis of molecular variance showed low genetic differentiation among all populations of P. pachyrhizi. The majority (> 90%) of the genetic diversity was distributed within each soyabean field, while approximately 6% of the genetic diversity was distributed among fields within geographic regions. Low population differentiation was indicated by the low FST values among populations, suggesting a wide dispersal of identical genotypes on a regional scale. Phylogenetic analysis indicated a strictly clonal structure of the populations and five main groups were observed, with group II accounting for 30% of the entire population. Because of the asexual reproduction of P. pachyrhizi, single-step mutations in SSR genotypes are likely to account for the genetic differences within each group. 2011-08 2017-10-05T07:42:43Z 2017-10-05T07:42:43Z Journal Article https://hdl.handle.net/10568/88162 en Limited Access Wiley Twizeyimana, M., Ojiambo, P.S., Haudenshield, J.S., Caetano‐Anollés, G., Pedley, K.F., Bandyopadhyay, R. & Hartman, G.L. (2011). Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean. Plant Pathology, 60(4), 719-729. |
| spellingShingle | glycine max soybeans virulence phenotypes Twizeyimana, M. Ojiambo, P.S. Haudenshield, J.S. Caetano‐Anollés, G. Pedley, K.F. Bandyopadhyay, Ranajit Hartman, G.L. Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean |
| title | Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean |
| title_full | Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean |
| title_fullStr | Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean |
| title_full_unstemmed | Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean |
| title_short | Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean |
| title_sort | genetic structure and diversity of phakopsora pachyrhizi isolates from soyabean |
| topic | glycine max soybeans virulence phenotypes |
| url | https://hdl.handle.net/10568/88162 |
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